Anti-SFPQ 抗体 [B92] (ab11825)
Key features and details
- Mouse monoclonal [B92] to SFPQ
- Suitable for: IHC-Fr, ICC/IF, ICC, WB, IP, RIA
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-SFPQ antibody [B92]
SFPQ 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [B92] to SFPQ -
由来種
Mouse -
アプリケーション
適用あり: IHC-Fr, ICC/IF, ICC, WB, IP, RIAmore details -
種交差性
交差種: Mouse, Human -
免疫原
Tissue, cells or virus corresponding to Mouse SFPQ.
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ポジティブ･コントロール
- Whole extract of cultured HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
B92 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11825の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 20082308
|
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| ICC |
Use at an assay dependent concentration.
(see Lee et al reference). |
|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 76 kDa.
(See Lee et al reference, Shav-Tal et al reference). |
| IP | (1) |
Use at an assay dependent concentration.
(see Lee et al reference, Carramolino et al reference). |
| RIA |
Use at an assay dependent concentration.
(see Lee et al reference). |
|
| AP |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. PubMed: 20082308 |
|
ICC
Use at an assay dependent concentration. (see Lee et al reference). |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 76 kDa. (See Lee et al reference, Shav-Tal et al reference). |
|
IP
Use at an assay dependent concentration. (see Lee et al reference, Carramolino et al reference). |
|
RIA
Use at an assay dependent concentration. (see Lee et al reference). |
|
AP
Use at an assay dependent concentration. |
ターゲット情報
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機能
DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity. -
関連疾患
Note=A chromosomal aberration involving SFPQ may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;1)(p11.2;p34) with TFE3. -
配列類似性
Contains 2 RRM (RNA recognition motif) domains. -
翻訳後修飾
The N-terminus is blocked.
Phosphorylated on multiple serine and threonine residues during apoptosis. In vitro phosphorylated by PKC. Phosphorylation stimulates binding to DNA and D-loop formation, but inhibits binding to RNA.
Arg-7, Arg-9, Arg-19 and Arg-25 are dimethylated, probably to asymmetric dimethylarginine. -
細胞内局在
Nucleus matrix. Predominantly in nuclear matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 6421 Human
- Entrez Gene: 71514 Mouse
- Omim: 605199 Human
- SwissProt: P23246 Human
- SwissProt: Q8VIJ6 Mouse
- Unigene: 355934 Human
- Unigene: 257276 Mouse
- Unigene: 482296 Mouse
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別名
- 100 kDa DNA pairing protein antibody
- 100 kDa DNA-pairing protein antibody
- 100 kDa subunit antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (23)
ab11825 は 23 報の論文で使用されています。
- Harley J et al. TDP-43 and FUS mislocalization in VCP mutant motor neurons is reversed by pharmacological inhibition of the VCP D2 ATPase domain. Brain Commun 3:fcab166 (2021). PubMed: 34396115
- Isobe M et al. Forced isoform switching of Neat1_1 to Neat1_2 leads to the loss of Neat1_1 and the hyperformation of paraspeckles but does not affect the development and growth of mice. RNA 26:251-264 (2020). PubMed: 31822595
- Zhang Y et al. Long non-coding RNA ARAP1-AS1 promotes tumorigenesis and metastasis through facilitating proto-oncogene c-Myc translation via dissociating PSF/PTB dimer in cervical cancer. Cancer Med 9:1855-1866 (2020). PubMed: 31953923
- Hou L et al. Concurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2. Nucleic Acids Res 48:3869-3887 (2020). PubMed: 32016422
- Noto PB et al. Identification of hnRNP-A1 as a pharmacodynamic biomarker of type I PRMT inhibition in blood and tumor tissues. Sci Rep 10:22155 (2020). PubMed: 33335114