Key features and details
- Goat polyclonal to SF3B3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
SF3B3 一次抗体 製品一覧
製品の詳細Goat polyclonal to SF3B3
アプリケーション適用あり: IHC-P, WBmore details
交差が予測される動物種: Mouse, Rat, Dog
- HeLa and Raji Lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab3608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 136 kDa).
1 hour primary incubation is recommended for this product.
機能Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
配列類似性Belongs to the RSE1 family.
- Information by UniProt
- KIAA0017 antibody
- Pre mRNA splicing factor SF3b 130 kDa subunit antibody
- Pre-mRNA-splicing factor SF3b 130 kDa subunit antibody
Anti-SF3B3 antibody (ab3608) at 0.1 µg/ml + HeLa cells, nuclear lysate at 30 µg
Predicted band size: 136 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
IHC image of ab3608 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3608, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab3608 は 2 報の論文で使用されています。
- Zhang Z et al. Synthesis and characterization of pseudocantharidins, novel phosphatase modulators that promote the inclusion of exon 7 into the survival of motoneuron (SMN) pre-mRNA. J Biol Chem : (2011). WB ; Human . PubMed: 21220421
- Yoshimoto R et al. Isolation and characterization of post-splicing lariat-intron complexes. Nucleic Acids Res : (2008). WB ; Human . PubMed: 19103666