Anti-Serum Amyloid P/SAP 抗体 (ab17872)

The reason why the customer is not seeing any bands is that normal brain has very very little SAP protein, therefore it is not surprising that the customer still sees no band. The reference below should be very useful, in particular, figure 3, which shows the levels of SAP in human brain lysate compared to an AD brain lysate: Human neurons generate C-reactive protein and amyloid P: upregulation in Alzheimer’s disease. Yasojima et al. Brain Research 887 (2000) 80–89. I therefore believe that the problem is not due to the antibody but to the low levels of protein in the customer's samples. I cannot offer a refund/credit note for this reason, Thank you for explaining this to your customer. Should she/he experience problems with a samples containing high levels of protein please do not hesitate to contact me again,

As Serum Amyloid P protein is only expressed in high levels in AD patients as is in low levels in normal subjects I think the problem is therefore low levels in the samples. Furthermore, the customer should try incubating in more concentrated antibody (still overnight) as the recommended range is 1/250-1/2500. I think it is essential for the customer to run a positive control to determine how high the levels of serum amyloid P protein are in their samples. Please do not hesitate to contact me for further assistance,

Thank you for contacting us for technical support on behalf of your customer. Many thanks for providing so much protocol information and the images of the blot. It enabled me to understand the problem very easily and rapidly. I am a little confused that the researcher seems different signals in the three experiments, are they the same samples, or different samples? I think the researcher's protocol is very good, I would expect the band size to be slightly lower than that seen by the monoclonal antibody that the researcher use. Is the researcher therefore confident that the higher band size detected by the monoclonal is the correct one? I am guessing that the serum samples are from AD patients, not control patients? I think the main problem is that the membrane is unfortunately blocked too much and the antibody added only for a short amount of time but in a very concentrated manner. I would therefore recommend, like in our lab, to incubate the membrane for 1 hour (it may be worth trying milk too as well as 5% BSA) and incubating the membrane overnight, probably in more diluted antibody (in TBST only). This will promote a slow but targeted binding of the antibody to the Serum Amyloid P protein. If the customer still experiences problems please do not hesitate to contact me again and I can offer a replacement vial or refund if the antibody was purchased in the last 90 days,

Good morning, Unfortunately the source of ab17872 does not have an image of the western blot to provide us. I am very sorry for this inconvenience, Best of luck with your research,