製品の概要

  • 製品名
    Anti-Serum Amyloid A antibody [115]
    Serum Amyloid A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [115] to Serum Amyloid A
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WB, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Highly purified recombinant human serum amyloid A (MW: 12 kDa).

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 6th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Use at an assay dependent dilution. This antibody, at a concentration of 0.1-0.5 µg/ml, will allow visualisation of 100 ng/lane of recombinant human SAA.
Sandwich ELISA Use at an assay dependent concentration.

sELISA compatible pair

ターゲット情報

  • 機能
    Major acute phase reactant. Apolipoprotein of the HDL complex.
  • 組織特異性
    Expressed by the liver; secreted in plasma.
  • 関連疾患
    Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.
    Note=Elevated serum SSA protein levels may be associated with lung cancer.
  • 配列類似性
    Belongs to the SAA family.
  • 翻訳後修飾
    This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Amyloid fibril protein AA antibody
      • Amyloid fibrils antibody
      • Amyloid protein A antibody
      • Fibrils antibody
      • MGC111216 antibody
      • OC antibody
      • PIG 4 antibody
      • PIG4 antibody
      • SAA 1 antibody
      • SAA 2 antibody
      • SAA antibody
      • SAA_HUMAN antibody
      • SAA1 antibody
      • SAA2 antibody
      • Serum amyloid A protein precursor antibody
      • Serum amyloid A1 isoform 1 antibody
      • Serum amyloid A1 isoform 2 antibody
      • Serum amyloid protein A(4-101) antibody
      • TP53I4 antibody
      • Tumor protein p53 inducible protein 4 antibody
      see all

    画像

    • Western blot - Anti-Serum Amyloid A antibody [115] (ab687)
      Western blot - Anti-Serum Amyloid A antibody [115] (ab687)Image courtesy of an anonymous Abreview.
      All lanes : Anti-Serum Amyloid A antibody [115] (ab687) at 1/4000 dilution

      Lanes 1-4 : Acute phase plasma samples
      Lane 5 : Recombinant C-SAA
      Lane 6 : Recombinant A-SAA

      Lysates/proteins at 14 µg per lane.

      Secondary
      All lanes : Goat anti-mouse IgG-HRP at 1/10000 dilution

      Developed using the ECL technique.

      Exposure time: 5 seconds
    • Western blot - Anti-Serum Amyloid A antibody [115] (ab687)
      Western blot - Anti-Serum Amyloid A antibody [115] (ab687)

    参考文献

    This product has been referenced in:
    • Heo SH  et al. Comparative proteomic analysis in children with idiopathic short stature (ISS) before and after short-term recombinant human growth hormone (rhGH) therapy. Proteomics 13:1211-9 (2013). Human . Read more (PubMed: 23436750) »
    • Jahangiri A  et al. Serum amyloid A is found on ApoB-containing lipoproteins in obese humans with diabetes. Obesity (Silver Spring) 21:993-6 (2013). Read more (PubMed: 23784902) »

    See all 5 Publications for this product

    ab687 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    17 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Serum Amyloid A antibody [115]

     Average
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako
    Permeabilization
    No
    Specification
    Liver
    Fixative
    Formaldehyde
    Read More
    Username

    Abcam user community

    Verified customer

    投稿 Sep 13 2016

    Blocking abreview for Anti-Serum Amyloid A antibody [115]

     Poor
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Blocking
    Sample
    Human Cultured Cells (monocytic cells)
    Specification
    monocytic cells
    Read More
    Username

    Abcam user community

    Verified customer

    投稿 Sep 12 2013

    Western blot abreview for Anti-Serum Amyloid A antibody [115]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Heart, Left Ventricle)
    Loading amount
    80 µg
    Specification
    Heart, Left Ventricle
    Gel Running Conditions
    Reduced Denaturing (10-20% gel)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    abreview image
    Username

    Dawn Bowles

    Verified customer

    投稿 Dec 28 2012

    I hope you are well.
    I am writing to follow up on the information I requested regarding immunogen sequencing of two anti-SAA antibodies (ab687 and ab18711) and their compatibility with the equine SAA molecule.
    Kind regards,

    Thank you for contacting us.

    I have now received further information from developer lab regarding the products ab687 and ab18711. Regrettably we will not be able to recommend these antibodies, to be used with horse samples. I apologies for any...

    Read More

    I hope you are well.

    I am writing to follow up on the information I requested regarding immunogen sequencing of two anti-SAA antibodies (ab687 and ab18711) and their compatibility with the equine SAA molecule.



    Kind regards,

    Thank you for your email.

    Finally, I have checked the sequence homology between human and horse SAA proteins, the homology is quite low with matching stretches.

    http://www.uniprot.org/blast/uniprot/2012101621EV411P6G?alignment=27 Read More

    I am interested in purchasing two monoclonal anti-serum amyloid A antibodies for the purpose of developing an assay.
    Can you tell me if the following antibodies are compatible pairs? ie. will steric hindrance occur on the target SAA molecule if bot...

    Read More

    Thank you for contacting us.

    We can recommend paring between ab687 and ab18711 instead of ab18713

    https://www.abcam.com/serum-Amyloid-A-antibody-607-ab18711.html

    https://www.abcam.com/Serum-Amyloid-A-antibody-115-ab687.html Read More

    Good morning,
    I wonder how much antibody (ab687) is specific for the gene SAA1,
    since western blot performed with recombinant SAA1, SAA2 and SAA4 has
    been able to identify all. What antibody for western blot assure you
    that is specif...

    Read More

    Thank you for contacting us. The antibody ab687 is reactive towards the Serum Amyloid A protein and not specific variants.. We do not have any antibodies specific for SAA1,SAA2 or SAA4.

    I hope this information is helpful to you. Please do not ...

    Read More

    Phone call requesting information on if anti-Serum Amyloid A antibodies ab687 and ab81483 are likely to work in native western blotting.

    Thank you for contacting us.

    I have looked into what applications anti-Serum Amyloid A antibodies ab687 and ab81483 have been used for in order to ascertain if they are likely to detect the native epitope of Serum Amyloid A in order to perfor...

    Read More

    ab1793: IHC-P image legend says "ab1793 staining TNF alpha in mouse liver tissue sections ... An undiluted HRP-conjugated monoclonal to rabbit IgG (1/200) was used as secondary antibody. " But the primary antibody is a mouse monoclonal Ab. will te...

    Read More

    Thank you for your phone call. 1) Regarding the image for ab1793, this was submitted by a customer as Abreview. For the details of the Abreview please follow this link: https://www.abcam.com/index.html?datasheet=1793&tab=abreviews&intabreviewid=1781...

    Read More

    Western blot abreview for Anti-Serum Amyloid A antibody [115]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (whole human acute phase plasma)
    Loading amount
    14 µg
    Specification
    whole human acute phase plasma
    Treatment
    heated to 95C in 10-fold excess of reducing SDS sample buffer for 10min
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Mar 21 2011

    1-10 of 17 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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