製品の概要

  • 製品名
    Anti-Serum Amyloid A antibody [115]
    Serum Amyloid A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [115] to Serum Amyloid A
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WB, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Highly purified recombinant human serum amyloid A (MW: 12 kDa).

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 6th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Use at an assay dependent dilution. This antibody, at a concentration of 0.1-0.5 µg/ml, will allow visualisation of 100 ng/lane of recombinant human SAA.
Sandwich ELISA Use at an assay dependent concentration.

sELISA compatible pair

ターゲット情報

  • 機能
    Major acute phase reactant. Apolipoprotein of the HDL complex.
  • 組織特異性
    Expressed by the liver; secreted in plasma.
  • 関連疾患
    Note=Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.
    Note=Elevated serum SSA protein levels may be associated with lung cancer.
  • 配列類似性
    Belongs to the SAA family.
  • 翻訳後修飾
    This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Amyloid fibril protein AA antibody
    • Amyloid fibrils antibody
    • Amyloid protein A antibody
    • Fibrils antibody
    • MGC111216 antibody
    • OC antibody
    • PIG 4 antibody
    • PIG4 antibody
    • SAA 1 antibody
    • SAA 2 antibody
    • SAA antibody
    • SAA_HUMAN antibody
    • SAA1 antibody
    • SAA2 antibody
    • Serum amyloid A protein precursor antibody
    • Serum amyloid A1 isoform 1 antibody
    • Serum amyloid A1 isoform 2 antibody
    • Serum amyloid protein A(4-101) antibody
    • TP53I4 antibody
    • Tumor protein p53 inducible protein 4 antibody
    see all

画像

  • All lanes : Anti-Serum Amyloid A antibody [115] (ab687) at 1/4000 dilution

    Lanes 1-4 : Acute phase plasma samples
    Lane 5 : Recombinant C-SAA
    Lane 6 : Recombinant A-SAA

    Lysates/proteins at 14 µg per lane.

    Secondary
    All lanes : Goat anti-mouse IgG-HRP at 1/10000 dilution

    Developed using the ECL technique.

    Exposure time: 5 seconds

参考文献

This product has been referenced in:
  • Heo SH  et al. Comparative proteomic analysis in children with idiopathic short stature (ISS) before and after short-term recombinant human growth hormone (rhGH) therapy. Proteomics 13:1211-9 (2013). Human . Read more (PubMed: 23436750) »
  • Jahangiri A  et al. Serum amyloid A is found on ApoB-containing lipoproteins in obese humans with diabetes. Obesity (Silver Spring) 21:993-6 (2013). Read more (PubMed: 23784902) »
See all 5 Publications for this product

レビューと Q&A

1-10 of 17 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako
Permeabilization
No
Specification
Liver
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Sep 13 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Blocking
Sample
Human Cultured Cells (monocytic cells)
Specification
monocytic cells

Abcam user community

Verified customer

投稿 Sep 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Heart, Left Ventricle)
Loading amount
80 µg
Specification
Heart, Left Ventricle
Gel Running Conditions
Reduced Denaturing (10-20% gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Dawn Bowles

Verified customer

投稿 Dec 28 2012

Answer

Thank you for contacting us.

I have now received further information from developer lab regarding the products ab687 and ab18711. Regrettably we will not be able to recommend these antibodies, to be used with horse samples. I apologies for any inconvenience.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your email.

Finally, I have checked the sequence homology between human and horse SAA proteins, the homology is quite low with matching stretches.

http://www.uniprot.org/blast/uniprot/2012101621EV411P6G?alignment=27


P0DJI9 is for human SSA protein and F6PJF6 is the ID for horse protein.

In order to further check whether the antibodies ab687 and ab18711 would be suitable, I have contacted the developer laboratory of these antibodies and asked if they can match the epitope of these antibodies with horse protein sequence. I will send you the details soon.

The other antibodies we have in catalogue are mostly against the full length human proteins so I am sorry we may not be able to support the horse species cross reactivity. We however have rabbit monoclonal that have >95% immunogen sequence homology with horse so this antibody should cross react with horse.

https://www.abcam.com/Serum-Amyloid-A-antibody-EPR4134-ab108992.html


Along with ab108992 you can search an anti SAA antibody which detects N-terminal end of protein, to use these antibodies in sELISA. I am sorry we do not have N-terminal specific products.


I hope this information will nevertheless helpful. Please do not hesitate to contact me with further inquiries.

Read More

Answer

Thank you for contacting us.

We can recommend paring between ab687 and ab18711 instead of ab18713

https://www.abcam.com/serum-Amyloid-A-antibody-607-ab18711.html

https://www.abcam.com/Serum-Amyloid-A-antibody-115-ab687.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us. The antibody ab687 is reactive towards the Serum Amyloid A protein and not specific variants.. We do not have any antibodies specific for SAA1,SAA2 or SAA4.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.

I have looked into what applications anti-Serum Amyloid A antibodies ab687 and ab81483 have been used for in order to ascertain if they are likely to detect the native epitope of Serum Amyloid A in order to perform native western blotting.

Both ab687 and ab81483 have been used in ELISA where the native epitope is presented. The ELISA carried out with ab687 was performed with the endogenously expressed protein, whilst the ELISA performed with ab81483 used recombinantly expressed protein. Both of these proteins are therefore likely to also be able to detect the protein when performing a native western blot. However, as we have not carried out this experiment I cannot guarantee that it will.

I also looked into what protocol had been used in performing immunoprecipitation with ab81483, however, the lab were unfortunately able to confirm that they had not tested the antibody in this application and that the informationhad been added to the datasheet in error. The datasheet has now been updated to reflect this.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Answer

Thank you for your phone call. 1) Regarding the image for ab1793, this was submitted by a customer as Abreview. For the details of the Abreview please follow this link: https://www.abcam.com/index.html?datasheet=1793&tab=abreviews&intabreviewid=17818 It might well be that the monoclonal rabbit IgG secondary antibody is part of the DAKO EnVision+ System, Peroxidase/DAB kit mentioned in the Abreview. But unfortunately, I do not have more information in this regard. ------------------------------------------------------------------------- 2) As for the testing discount for ab687, here is the necessary information: DISCOUNT CODE: xxx Expiration date: xxx I am very pleased to hear you would like to accept our offer and test ab687 in mouse. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. I hope this information is of help to you. Please let me know if I can assist you further with our products.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (whole human acute phase plasma)
Loading amount
14 µg
Specification
whole human acute phase plasma
Treatment
heated to 95C in 10-fold excess of reducing SDS sample buffer for 10min
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Mar 21 2011

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録