リコンビナントRabMAb

Anti-Serpin A5 抗体 [EPR23337-112] - BSA and Azide free (ab272396)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-112] - BSA and Azide free (ab272396)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-112] - BSA and Azide free (ab272396)
  • Anti-Serpin A5 antibody [EPR23337-112] - BSA and Azide free (ab272396)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23337-112] to Serpin A5 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-Serpin A5 antibody [EPR23337-112] - BSA and Azide free
    Serpin A5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23337-112] to Serpin A5 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human serum and plasma; Rat serum and plasma; Mouse serum and plasma. IHC-P: Human kidney and testis tissue.

  • 特記事項

    This antibody (EPR23337-112), is a different clone to ab270450 (EPR23337-69).

    ab272396 is the carrier-free version of ab270451. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-P
Human
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr or IP.

    • ターゲット情報

    • 機能

      Inhibits activated protein C as well as plasminogen activators.

    • 組織特異性

      Expressed by the liver and secreted in plasma.

    • 配列類似性

      Belongs to the serpin family.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Acrosomal serine protease inhibitor antibody
        • IPSP antibody
        • IPSP_HUMAN antibody
        • PAI 3 antibody
        • PAI-3 antibody
        • PAI3 antibody
        • PCI antibody
        • PCI-B antibody
        • PLANH 3 antibody
        • PLANH3 antibody
        • Plasma serine protease inhibitor antibody
        • Plasminogen activator inhibitor 3 antibody
        • Plasminogen activator inhibitor III antibody
        • PROCI antibody
        • Protein C inhibitor antibody
        • Serpin A5 antibody
        • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 5 antibody
        • Serpin peptidase inhibitor clade A member 5 antibody
        • Serpina5 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-112] - BSA and Azide free (ab272396)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-112] - BSA and Azide free (ab272396)

        Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Serpin A5 with ab270451 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on proximal tubules of human kidney (PMID: 14696115). The section was incubated with ab270451 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270451).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-112] - BSA and Azide free (ab272396)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-112] - BSA and Azide free (ab272396)

        Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Serpin A5 with ab270451 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human testis (PMID: 1372913). The section was incubated with ab270451 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270451).

      • Anti-Serpin A5 antibody [EPR23337-112] - BSA and Azide free (ab272396)
        Anti-Serpin A5 antibody [EPR23337-112] - BSA and Azide free (ab272396)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab272396 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab272396 は論文での使用が確認できていません。

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