Anti-Serotonin transporter 抗体 (ab172884)
Key features and details
- Rabbit polyclonal to Serotonin transporter
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
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製品名
Anti-Serotonin transporter antibody
Serotonin transporter 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Serotonin transporter -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Sheep, Cow, Human, Chimpanzee, Macaque monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Rat Serotonin transporter aa 200-300 conjugated to keyhole limpet haemocyanin.
Database link: P31652 -
ポジティブ・コントロール
- This antibody gave a positive signal in the following tissue lysates: Mouse Serotinergic Nucleus; Rat Serotinergic Nucleus; Mouse Substantia Nigra; Rat Substantia Nigra; Rat Cerebellum.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab172884の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 60,62 kDa (predicted molecular weight: 70 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 60,62 kDa (predicted molecular weight: 70 kDa). |
ターゲット情報
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機能
Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner. -
組織特異性
Expressed in platelets (at protein level). -
配列類似性
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily. -
翻訳後修飾
Glycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms.
Phosphorylated at Ser-611, Thr-613 and Thr-616. -
細胞内局在
Cell membrane. Endomembrane system. Endosome membrane. Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin. - Information by UniProt
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参照データベース
- Entrez Gene: 282365 Cow
- Entrez Gene: 6532 Human
- Entrez Gene: 15567 Mouse
- Entrez Gene: 25553 Rat
- Entrez Gene: 443491 Sheep
- Omim: 182138 Human
- SwissProt: Q9XT49 Cow
- SwissProt: P31645 Human
see all -
別名
- 5 HTT antibody
- 5 HTTLPR antibody
- 5 hydroxytryptamine (serotonin) transporter antibody
see all
画像
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All lanes : Anti-Serotonin transporter antibody (ab172884) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Mouse Serotinergic Nucleus Tissue Lysate
Lane 2 : Rat Serotinergic Nucleus Tissue Lysate
Lane 3 : Mouse Substantia Nigra Tissue Lysate
Lane 4 : Rat Substantia Nigra Tissue Lysate
Lane 5 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 60,62 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab172884 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab172884 は 3 報の論文で使用されています。
- Li C et al. Modulation of depression-related behaviors by adiponectin AdipoR1 receptors in 5-HT neurons. Mol Psychiatry N/A:N/A (2020). PubMed: 31980728
- Han X et al. Electroacupuncture restores hippocampal synaptic plasticity via modulation of 5-HT receptors in a rat model of depression. Brain Res Bull 139:256-262 (2018). WB . PubMed: 29524471
- Lin WH et al. Novel insights into the effect of paroxetine administration in pilocarpine-induced chronic epileptic rats. Mol Med Rep 16:8245-8252 (2017). PubMed: 28983622