Serotonin ELISA Kit (ab133053)
Key features and details
- Sensitivity: 0.293 ng/ml
- Range: 0.49 ng/ml - 500 ng/ml
- Sample type: Plasma, Platelets, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Species independent
製品の概要
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製品名
Serotonin ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% 346.1 ng/ml 20 4.2% 53.8 ng/ml 20 5.8% 13.5 ng/ml 20 11% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% 358.0 ng/ml 16.2% 53.9 ng/ml 18.4% 13.2 ng/ml 12.7% -
サンプルの種類
Urine, Serum, Plasma, Platelets -
アッセイタイプ
Competitive -
検出感度
0.293 ng/ml -
検出範囲
0.49 ng/ml - 500 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Urine 101 5ng/ml - 100ng/ml Serum 91 5ng/ml - 100ng/ml Platelets 107 5ng/ml - 100ng/ml Cit plasma 95 5ng/ml - 100ng/ml -
全工程の試験時間
3h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Species independent -
製品の概要
Serotonin ELISA kit is a competitive ELISA kit designed for the accurate quantitative measurement of Serotonin in Human Serum, Platelets, Plasma and Urine.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Serotonin antigen and a polyclonal rabbit antibody specific to Serotonin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Serotonin captured in the plate.
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特記事項
Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine found in the central nervous system, gastrointestinal tract, and blood with broad physiological functions as a neurotransmitter, in gastric motility, hemostasis, and cardiovascular integrity. Defects in serotonin signaling have been linked to a large number of complex behavioral disorders in humans, including anxiety and depression, autism, and eating disorders, and to neurodegenerative disorders such as Parkinson’s. Platelets serve as the major reservoir of serotonin in the bloodstream. When activated, platelets release serotonin into the bloodstream where it acts as a powerful vasoconstrictor. Treatment with Selective Serotonin Uptake Inhibitors (SSRIs) dramatically reduces platelet serotonin concentrations, and altered levels of serotonin in the circulatory system are implicated in such diverse conditions such as asthma, liver cirrhosis and carcinoid tumors.
Cross reactivities:
Compound Cross Reactivity N-Acetyl Serotonin 17% 5-Hydroxy-L-tryptophan 0.4% Tryptamine 0.1% 5-Hydroxyindoleacetic acid 0.03% Melatonin 0.01% Tyramine <0.004% Tryptophan <0.004% -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 1 x 27ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 2 units pNpp Substrate 1 x 20ml Serotonin Alkaline Phosphatase Conjugate 2 x 150ng Serotonin Antibody 2 vials Serotonin Standard 2 x 250ng Stop Solution 1 x 5ml -
研究分野
画像
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Serotonin measured (averages of undil., 1:3, 1:9 & 1:27) in biological fluids (Neuro2a an Growth med. averaged from 1:2 and 1:4) showing quantity (ng) per mL of tested sample.
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Representative Standard Curve using ab133053.
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Dose-response curves from Human platelets and Human serum diluted into assay buffer were compared to the Serotonin standard curve. The parallel response indicates the standard effectively mimics the native protein.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (28)
ab133053 は 28 報の論文で使用されています。
- Kim KH & Lee KA Metabolic Rewiring by Human Placenta-Derived Mesenchymal Stem Cell Therapy Promotes Rejuvenation in Aged Female Rats. Int J Mol Sci 23:N/A (2022). PubMed: 35008991
- Wauquier F et al. Circulating Human Serum Metabolites Derived from the Intake of a Saffron Extract (Safr'InsideTM) Protect Neurons from Oxidative Stress: Consideration for Depressive Disorders. Nutrients 14:N/A (2022). PubMed: 35406124
- Wang J et al. Resveratrol Alleviating the Ovarian Function Under Oxidative Stress by Alternating Microbiota Related Tryptophan-Kynurenine Pathway. Front Immunol 13:911381 (2022). PubMed: 35911670
- Piancone F et al. Modulation of Neuroendocrine and Immunological Biomarkers Following Rehabilitation in Sarcopenic Patients. Cells 11:N/A (2022). PubMed: 36010554
- Bui BP et al. Anxiolytic effect of Korean Red Ginseng through upregulation of serotonin and GABA transmission and BDNF expression in immobilized mice. J Ginseng Res 46:819-829 (2022). PubMed: 36312738