Anti-Serotonin 抗体 [YC5/45] (ab6336)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)](https://www.abcam.co.jp/ps/products/6/ab6336/Images/ab6336_4.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)")
Key features and details
- Rat monoclonal [YC5/45] to Serotonin
- Suitable for: IHC-Fr, IHC-P, ICC/IF, IHC-FoFr
- Reacts with: Species independent
- Isotype: IgG2c
製品の概要
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製品名
Anti-Serotonin antibody [YC5/45]
Serotonin 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [YC5/45] to Serotonin -
由来種
Rat -
アプリケーション
適用あり: IHC-Fr, IHC-P, ICC/IF, IHC-FoFrmore details -
種交差性
交差種: Species independent -
免疫原
5HT-bovine seroalbumin.
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ポジティブ･コントロール
- In IHC, this antibody gave a positive signal in a formalin-fixed, paraffin-embedded coronal section of Mouse Brain.
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特記事項
Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Tissue culture supernatant -
一次抗体 備考
Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system. -
ポリ/モノ
モノクローナル -
クローン名
YC5/45 -
アイソタイプ
IgG2c -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab6336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | Use a concentration of 1 - 5 µg/ml. (see Ni et al). Fix with ice cold acetone. Dilute antibody in 1.5% blocking serum. | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-FoFr | 1/100 - 1/1000. Used on gelatin embedded perfusion fixed tissue (see recommended protocol). |
ターゲット情報
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関連性
Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20µm) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors. -
細胞内局在
Cytoplasmic and Secreted -
別名
- 5 hydroxytryptamine antibody
- 5HT antibody
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
ab6336 in rat brain tissue fixed with 4% paraformaldehyde. The staining is appropriate, lighting up cell bodies, dendrites and terminals of known 5-HT containing neurones. These pictures are from the review provided by Jim Deuchars.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)](https://www.abcam.co.jp/ps/products/6/ab6336/Images/ab6336-12285-anti-serotonin-antibody-yc5-45-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)IHC image of Serotonin staining in mouse brain (coronal) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6336, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody [YC5/45] (ab6336)](https://www.abcam.co.jp/ps/products/6/ab6336/Images/ab6336-305083-anti-serotonin-antibody-yc5-45-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody [YC5/45] (ab6336)This image is courtesy of Ms. Szu-yu Liu by an abreviewParaformaldehyde-fixed rabbit spinal cord tissue stained for Serotonin using ab6336 at 1/200 dilution in immunohistochemical analysis. Power Block™ Universal Blocking Reagen t(Biogenex) was used as ablocking agent for 10 minute(s) at a concentration of 10% at 37°C. Biotin-SP-conjugated Goat Anti-Rat IgG was used as the secondary antibody at 1/500 dilution.
データシートおよび資料
参考文献 (15)
ab6336 は 15 報の論文で使用されています。
- Zhang W et al. Wheel Running Improves Motor Function and Spinal Cord Plasticity in Mice With Genetic Absence of the Corticospinal Tract. Front Cell Neurosci 13:106 (2019). PubMed: 30941019
- Humenick A et al. Characterization of projections of longitudinal muscle motor neurons in human colon. Neurogastroenterol Motil 31:e13685 (2019). PubMed: 31355986
- König D et al. Distribution and Restoration of Serotonin-Immunoreactive Paraneuronal Cells During Caudal Fin Regeneration in Zebrafish. Front Mol Neurosci 12:227 (2019). PubMed: 31616250
- Sitaraman D et al. Discrete Serotonin Systems Mediate Memory Enhancement and Escape Latencies after Unpredicted Aversive Experience inDrosophilaPlace Memory. Front Syst Neurosci 11:92 (2017). PubMed: 29321732
- Dhillon RS et al. Axonal plasticity underpins the functional recovery following surgical decompression in a rat model of cervical spondylotic myelopathy. Acta Neuropathol Commun 4:89 (2016). IHC-Fr . PubMed: 27552807
