Anti-Serotonin 抗体 [YC5/45] (ab6336)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)](https://www.abcam.co.jp/ps/products/6/ab6336/Images/ab6336_4.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)")
Key features and details
- Rat monoclonal [YC5/45] to Serotonin
- Suitable for: IHC-Fr, IHC-P, ICC/IF, IHC-FoFr
- Reacts with: Species independent
- Isotype: IgG2c
製品の概要
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製品名
Anti-Serotonin antibody [YC5/45]
Serotonin 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [YC5/45] to Serotonin -
由来種
Rat -
アプリケーション
適用あり: IHC-Fr, IHC-P, ICC/IF, IHC-FoFrmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Serotonin conjugated to bovine serum albumin.
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ポジティブ･コントロール
- In IHC, this antibody gave a positive signal in a formalin-fixed, paraffin-embedded coronal section of Mouse Brain.
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特記事項
Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Tissue culture supernatant -
一次抗体 備考
Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system. -
ポリ/モノ
モノクローナル -
クローン名
YC5/45 -
アイソタイプ
IgG2c -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6336の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | (1) |
Use a concentration of 1 - 5 µg/ml. (see Ni et al). Fix with ice cold acetone. Dilute antibody in 1.5% blocking serum.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| IHC-FoFr |
1/100 - 1/1000. Used on gelatin embedded perfusion fixed tissue (see recommended protocol).
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| 特記事項 |
|---|
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IHC-Fr
Use a concentration of 1 - 5 µg/ml. (see Ni et al). Fix with ice cold acetone. Dilute antibody in 1.5% blocking serum. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-FoFr
1/100 - 1/1000. Used on gelatin embedded perfusion fixed tissue (see recommended protocol). |
ターゲット情報
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関連性
Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20µm) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors. -
細胞内局在
Cytoplasmic and Secreted -
別名
- 5 hydroxytryptamine antibody
- 5HT antibody
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
ab6336 in rat brain tissue fixed with 4% paraformaldehyde. The staining is appropriate, lighting up cell bodies, dendrites and terminals of known 5-HT containing neurones. These pictures are from the review provided by Jim Deuchars.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)](https://www.abcam.co.jp/ps/products/6/ab6336/Images/ab6336-12285-anti-serotonin-antibody-yc5-45-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)IHC image of Serotonin staining in mouse brain (coronal) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6336, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody [YC5/45] (ab6336)](https://www.abcam.co.jp/ps/products/6/ab6336/Images/ab6336-305083-anti-serotonin-antibody-yc5-45-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody [YC5/45] (ab6336)This image is courtesy of Ms. Szu-yu Liu by an abreviewParaformaldehyde-fixed rabbit spinal cord tissue stained for Serotonin using ab6336 at 1/200 dilution in immunohistochemical analysis. Power Block™ Universal Blocking Reagen t(Biogenex) was used as ablocking agent for 10 minute(s) at a concentration of 10% at 37°C. Biotin-SP-conjugated Goat Anti-Rat IgG was used as the secondary antibody at 1/500 dilution.
データシートおよび資料
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Datasheet download
参考文献 (19)
ab6336 は 19 報の論文で使用されています。
- Liu H et al. Blunted diurnal firing in lateral habenula projections to dorsal raphe nucleus and delayed photoentrainment in stress-susceptible mice. PLoS Biol 19:e3000709 (2021). PubMed: 33690628
- Fields AM et al. Vitamin B6 deficiency disrupts serotonin signaling in pancreatic islets and induces gestational diabetes in mice. Commun Biol 4:421 (2021). PubMed: 33772108
- Mao J et al. Bisphenol A and bisphenol S disruptions of the mouse placenta and potential effects on the placenta-brain axis. Proc Natl Acad Sci U S A 117:4642-4652 (2020). PubMed: 32071231
- Zhang T et al. APC mutations in human colon lead to decreased neuroendocrine maturation of ALDH+ stem cells that alters GLP-2 and SST feedback signaling: Clue to a link between WNT and retinoic acid signalling in colon cancer development. PLoS One 15:e0239601 (2020). PubMed: 33112876
- König D et al. Distribution and Restoration of Serotonin-Immunoreactive Paraneuronal Cells During Caudal Fin Regeneration in Zebrafish. Front Mol Neurosci 12:227 (2019). PubMed: 31616250
