Anti-Serotonin 抗体 [YC5/45] (ab6336)

Reviews (2)Q&A (6)References (16)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
  • Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody [YC5/45] (ab6336)

Key features and details

  • Rat monoclonal [YC5/45] to Serotonin
  • Suitable for: IHC-Fr, IHC-P, ICC/IF, IHC-FoFr
  • Reacts with: Species independent
  • Isotype: IgG2c

製品の概要

  • 製品名

    Anti-Serotonin antibody [YC5/45]
    Serotonin 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [YC5/45] to Serotonin

  • 由来種

    Rat

  • アプリケーション

    適用あり: IHC-Fr, IHC-P, ICC/IF, IHC-FoFrmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    5HT-bovine seroalbumin.

  • ポジティブ･コントロール

    • In IHC, this antibody gave a positive signal in a formalin-fixed, paraffin-embedded coronal section of Mouse Brain.

  • 特記事項


    Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • 一次抗体 備考

    Useful for the diagnosis of carcinoid tumours and the investigation of the effects of drugs on the neurological system.

  • ポリ/モノ

    モノクローナル

  • クローン名

    YC5/45

  • アイソタイプ

    IgG2c

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6336の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr (1)
Use a concentration of 1 - 5 µg/ml. (see Ni et al). Fix with ice cold acetone. Dilute antibody in 1.5% blocking serum.
IHC-P (1)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-FoFr
1/100 - 1/1000. Used on gelatin embedded perfusion fixed tissue (see recommended protocol).
特記事項
IHC-Fr
Use a concentration of 1 - 5 µg/ml. (see Ni et al). Fix with ice cold acetone. Dilute antibody in 1.5% blocking serum.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-FoFr
1/100 - 1/1000. Used on gelatin embedded perfusion fixed tissue (see recommended protocol).

ターゲット情報

  • 関連性

    Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20µm) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors.

  • 細胞内局在

    Cytoplasmic and Secreted

  • 別名

    • 5 hydroxytryptamine antibody
    • 5HT antibody

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)

    ab6336 in rat brain tissue fixed with 4% paraformaldehyde. The staining is appropriate, lighting up cell bodies, dendrites and terminals of known 5-HT containing neurones. These pictures are from the review provided by Jim Deuchars.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
    IHC image of Serotonin staining in mouse brain (coronal) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6336, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody [YC5/45] (ab6336)
    Immunohistochemistry (Frozen sections) - Anti-Serotonin antibody [YC5/45] (ab6336)This image is courtesy of Ms. Szu-yu Liu by an abreview

    Paraformaldehyde-fixed rabbit spinal cord tissue stained for Serotonin using ab6336 at 1/200 dilution in immunohistochemical analysis. Power Block™ Universal Blocking Reagen t(Biogenex) was used as ablocking agent for 10 minute(s) at a concentration of 10% at 37°C. Biotin-SP-conjugated Goat Anti-Rat IgG was used as the secondary antibody at 1/500 dilution.

    See Abreview

参考文献 (16)

ab6336 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6336 は 16 報の論文で使用されています。

  • Zhang T  et al. APC mutations in human colon lead to decreased neuroendocrine maturation of ALDH+ stem cells that alters GLP-2 and SST feedback signaling: Clue to a link between WNT and retinoic acid signalling in colon cancer development. PLoS One 15:e0239601 (2020). PubMed: 33112876
  • Zhang W  et al. Wheel Running Improves Motor Function and Spinal Cord Plasticity in Mice With Genetic Absence of the Corticospinal Tract. Front Cell Neurosci 13:106 (2019). PubMed: 30941019
  • Humenick A  et al. Characterization of projections of longitudinal muscle motor neurons in human colon. Neurogastroenterol Motil 31:e13685 (2019). PubMed: 31355986
  • König D  et al. Distribution and Restoration of Serotonin-Immunoreactive Paraneuronal Cells During Caudal Fin Regeneration in Zebrafish. Front Mol Neurosci 12:227 (2019). PubMed: 31616250
  • Sitaraman D  et al. Discrete Serotonin Systems Mediate Memory Enhancement and Escape Latencies after Unpredicted Aversive Experience inDrosophilaPlace Memory. Front Syst Neurosci 11:92 (2017). PubMed: 29321732
View all Publications for this product

レビューと Q&A

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1-8 of 8 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Serotonin antibody [YC5/45]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
Permeabilization
Yes - TritonX100 0.5%
Specification
colon
Blocking step
Dako Blocking solution as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formalin 4%
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Abcam user community

Verified customer

投稿 Dec 16 2020

Immunohistochemistry (Frozen sections) abreview for Anti-Serotonin antibody [YC5/45]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Power Block™ Universal Blocking Reagent(Biogenex) as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 37°C
Read More

Ms. Szu-yu Liu

Verified customer

投稿 Nov 23 2012

Question

Product code: 6336
Inquiry: Dear abcam technical support team: This customer would like to order ab6336 (Anti-Serotonin antibody [YC5/45]) antibody) and test in IHC-Fr with rabbit sample, would you please help this customer to create a discount code, the information of this customer as follow: xxxxxxx
Could you please help this customer to solve this problem? Thanks for your kindly help. Best regards

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Answer

Thank you for contacting us.

The anitbody ab6336 has been tested in IHC-Fr but as yet has not been used with rabbit samples. This is therefore information that we would be very interested in. I have therefore generated the discount code as requested which is stated below:

Discount code: xxxxxxx

Expiration date: 24th October 2012

If you have any further questions please do not hesitate to ask.

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Question

I'm interested in your rat anti-serotonin monoclonal antibody (YC5/45); code ab6336. However It is in the culture supernant form. Could you tell me what is the concentration of the antibody? Thanks! Have a nice holiday!

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Answer

Thank you for your enquiry. Ab6336 is in an unpurified form, tissue culture supernatant, and we cannnot guarantee the concentration for unpurified material.

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Question

Do you have antibody to serotonin transporter for cats?

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Answer

Thank you for your enquiry. I did a search in our online catalog and the following antibodies are the serotonin antibodies which we currently have available: ab3439, ab3440, ab6444, ab3505, ab3506, ab6336, ab8882, ab2403, and ab949. Unfortunately, none of them have yet been tested for cross-reactivity in cats. Should you decide to go ahead and purchase any of these products, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. If you have any more questions, please contact us again.

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Question

Antibody requested: 5HT2B, 5HT2C, 5HT1B (1) are these clones of Serotonin? (2) what company has them available?

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Answer

We produce some of our antibodies in-house and others are sourced from suppliers whose products we believe will be of interest to our customers. The identity of our suppliers remains commercially sensitive information.

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Question

I have tested this antibody on formaldehyde fixed Xenopus laevis whole embryos (stages 28 to 34) and I have not detected a reliable signal. I used concentrations of 1:1, 1:10 and 1:20. Do you have any information on the use of this antibody with Xenopus samples?

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Answer

The serotonin antibody was tested on mature frog tissue.

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Question

I would like to lable serotinergic cells in chicken brain on cryosections (perhaps Paraffin) with fluorescence. Can you recomand this antibody for this purpose?

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Answer

So far, this antibody has not been tested on chicken samples.

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