Anti-SERCA2 ATPase 抗体 [IID8] (ab2817)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [IID8] to SERCA2 ATPase
- Suitable for: WB, IHC-P
- Reacts with: Human
製品の概要
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製品名
Anti-SERCA2 ATPase antibody [IID8]
SERCA2 ATPase 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [IID8] to SERCA2 ATPase -
由来種
Mouse -
特異性
Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 2 (SERCA2) ATPase. This antibody does not discriminate between the two isoforms. By Western blot, this antibody detects an ~110 kDa protein representing SERCA2 ATPase from canine skeletal muscle triad preparations. Immunofluorescence staining of SECRA2 ATPase in rabbit skeletal muscle results in strong labeling of the entire type I (slow) myofiber consistent with sarcoplasmic reticulum localization. This antibody is not recommended for Western blot detection of rat SERCA2. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) corresponding to Dog SERCA2 ATPase. Purified canine cardiac sarcoplasmic reticulum.
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ポジティブ・コントロール
- IHC-P: Human skeletal muscle and cardiac muscle tissue. WB: Human skeletal muscle. HeLa, HepG2, A549 and A673 whole cell lysate.
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特記事項
This product has switched from a hybridoma to recombinant production method on 25th November 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
一次抗体 備考
ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells. -
ポリ/モノ
モノクローナル -
クローン名
IID8 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab2817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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WB | 1/1000. Detects a band of approximately 100 kDa. | |
IHC-P | 1/4000. |
ターゲット情報
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機能
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle. -
組織特異性
Isoform 1 is widely expressed in smooth muscle and nonmuscle tissues such as in adult skin epidermis, with highest expression in liver, pancreas and lung, and intermediate expression in brain, kidney and placenta. Also expressed at lower levels in heart and skeletal muscle. Isoforms 2 and 3 are highly expressed in the heart and slow twitch skeletal muscle. Expression of isoform 3 is predominantly restricted to cardiomyocytes and in close proximity to the sarcolemma. Both isoforms are mildly expressed in lung, kidney, liver, pancreas and placenta. Expression of isoform 3 is amplified during monocytic differentiation and also observed in the fetal heart. -
関連疾患
Defects in ATP2A2 are a cause of acrokeratosis verruciformis (AKV) [MIM:101900]; also known as Hopf disease. AKV is a localized disorder of keratinization, which is inherited as an autosomal dominant trait. Its onset is early in life with multiple flat-topped, flesh-colored papules on the hands and feet, punctate keratoses on the palms and soles, with varying degrees of nail involvement. The histopathology shows a distinctive pattern of epidermal features with hyperkeratosis, hypergranulosis, and acanthosis together with papillomatosis. These changes are frequently associated with circumscribed elevations of the epidermis that are said to resemble church spires. There are no features of dyskeratosis or acantholysis, the typical findings in lesions of Darier disease.
Defects in ATP2A2 are the cause of Darier disease (DD) [MIM:124200]; also known as Darier-White disease (DAR). DD is an autosomal dominantly inherited skin disorder characterized by loss of adhesion between epidermal cells (acantholysis) and abnormal keratinization. Patients with mild disease may have no more than a few scattered keratotic papules or subtle nail changes, whereas those with severe disease are handicapped by widespread malodorous keratotic plaques. In a few families, neuropsychiatric abnormalities such as mild mental retardation, schizophrenia, bipolar disorder and epilepsy have been reported. Stress, UV exposure, heat, sweat, friction, and oral contraception exacerbate disease symptoms. Prevalence has been estimated at 1 in 50000. Clinical variants of DD include hypertrophic, vesicobullous, hypopigmented, cornifying, zosteriform or linear, acute and comedonal subtypes. Comedonal Darier disease (CDD) is characterized by the coexistence of acne-like comedonal lesions with typical Darier hyperkeratotic papules on light-exposed areas. At histopathologic level, CDD differs from classic DD in the prominent follicular involvement and the presence of greatly elongated dermal villi. -
配列類似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. -
翻訳後修飾
Nitrated under oxidative stress. Nitration on the two tyrosine residues inhibits catalytic activity. -
細胞内局在
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 488 Human
- Omim: 108740 Human
- SwissProt: P16615 Human
- Unigene: 506759 Human
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別名
- AT2A2_HUMAN antibody
- Atp2a2 antibody
- ATP2B antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [IID8] (ab2817)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling SERCA2 ATPase with ab2817 at 1/4000 dilution, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human skeletal muscle tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [IID8] (ab2817)
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling SERCA2 ATPase with ab2817 at 1/4000 dilution, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human cardiac muscle tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
All lanes : Anti-SERCA2 ATPase antibody [IID8] (ab2817) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 3 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lane 4 : A673 (human muscle Ewing's Sarcoma), whole cell lysate
Lane 5 : Human skeletal muscle
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-2: 92 seconds; Lanes 3-4: 10 seconds; Lane 5: 3 seconds.
Samples are non-boiled as boiling may cause protein aggregates.
プロトコール
データシートおよび資料
参考文献 (28)
ab2817 は 28 報の論文で使用されています。
- Prado NJ et al. Reperfusion Arrhythmias Increase after Superior Cervical Ganglionectomy Due to Conduction Disorders and Changes in Repolarization. Int J Mol Sci 21:N/A (2020). PubMed: 32155697
- Nagendran T & Taylor AM Unique Axon-to-Soma Signaling Pathways Mediate Dendritic Spine Loss and Hyper-Excitability Post-axotomy. Front Cell Neurosci 13:431 (2019). PubMed: 31607869
- Cao Q et al. Dickkopf-3 upregulation mediates the cardioprotective effects of curcumin on chronic heart failure. Mol Med Rep 17:7249-7257 (2018). PubMed: 29568962
- Sun Z et al. Exendin-4 Plays a Protective Role in a Rat Model of Spinal Cord Injury Through SERCA2. Cell Physiol Biochem 47:617-629 (2018). PubMed: 29794460
- da Silva JS et al. Adenosine A2A receptor agonist prevents cardiac remodeling and dysfunction in spontaneously hypertensive male rats after myocardial infarction. Drug Des Devel Ther 11:553-562 (2017). IHC-P ; Rat . PubMed: 28293100