Application
Western blot
Sample
Rabbit Tissue lysate - other (muscle and heart)
Gel Running Conditions
Reduced Denaturing (4-12 %)
Loading amount
10 µg
Specification
muscle and heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBSTweenbuffer
Dilution
1/5000
Secondary antibody
Dilution
1/25000
Name
Non-Abcam antibody was used: Donkey Anti-rabbit igg
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL Prime
Negative control
Liver
Exposure
30 second(s)
Bands
Specific: 100 kDa Non-specific: 70 kDa
Positive control
none
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MRS. Minh Hien Le
Verified customer
投稿 Nov 25 2015