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Anti-SERCA1 ATPase 抗体 [IIH11] - BSA and Azide free (ab255961)

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Western blot - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)
  • Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [IIH11] to SERCA1 ATPase - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free
    SERCA1 ATPase 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [IIH11] to SERCA1 ATPase - BSA and Azide free

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • エピトープ

    This antibody recognizes an epitope between amino acids 199-505 of rabbit skeletal muscle ATPase, a region that is not exposed in native sarcoplasmic reticulum.

  • ポジティブ･コントロール

    • WB: Mouse and rat skeletal muscle tissue lysate. IHC-P: Mouse and rat skeletal muscle tissue.

  • 特記事項

    ab255961 is the carrier-free version of ab2818.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

関連製品

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab255961の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 0.466 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
IHC-P
Use a concentration of 0.117 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
Use a concentration of 0.466 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
IHC-P
Use a concentration of 0.117 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.

  • 組織特異性

    Skeletal muscle, fast twitch muscle (type II) fibers.

  • 関連疾患

    Brody myopathy

  • 配列類似性

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.

  • 発生段階

    Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle.

  • 細胞内局在

    Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • fast twitch skeletal muscle isoform antibody
      • AT2A1_HUMAN antibody
      • ATP2A antibody
      • ATP2A1 antibody
      • ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody
      • ATPase Ca++ transporting fast twitch 1 antibody
      • ATPase, Ca(2+)-transporting fast twitch 1 antibody
      • Calcium pump 1 antibody
      • Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody
      • Calcium-transporting ATPase sarcoplasmic reticulum type antibody
      • EC 3.6.3.8 antibody
      • Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody
      • Fast skeletal muscle SR calcium ATPase antibody
      • OTTHUMP00000162561 antibody
      • OTTHUMP00000162562 antibody
      • Sarcoendoplasmic reticulum calcium ATPase antibody
      • Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody
      • Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody
      • SERCA 1 antibody
      • SERCA1 antibody
      • SERCA1 truncated isoform, included antibody
      • SR Ca(2+) ATPase 1 antibody
      • SR Ca(2+)-ATPase 1 antibody
      see all

    画像

    • Western blot - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)
      Western blot - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)
      All lanes : Anti-SERCA1 ATPase antibody [IIH11] (ab2818) at 1/1000 dilution

      Lane 1 : Mouse skeletal muscle tissue lysate
      Lane 2 : Rat skeletal muscle tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L), at 1/10000 dilution

      Predicted band size: 110 kDa


      Exposure time: 48 seconds


      This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab2818).

      Blocking/Dilution buffer: 5% NFDM/TBST.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)

      This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab2818).

      Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling SERCA1 ATPase with ab2818 at 0.117µg/ml, followed by ready to use secondary. Cytoplasmic staining on rat skeletal muscle tissue is observed. The section was incubated with ab2818 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)

      This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab2818).

      Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling SERCA1 ATPase with ab2818 at 0.117µg/ml, followed by ready to use secondary. Cytoplasmic staining on mouse skeletal muscle tissue is observed. The section was incubated with ab2818 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 mins.

    • Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)
      Anti-SERCA1 ATPase antibody [IIH11] - BSA and Azide free (ab255961)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab255961 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab255961 は論文での使用が確認できていません。

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