Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (C2C12)
Specification
C2C12
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 0.1% Triton X-100, 0.5% serum
Secondary antibody
Name
Non-Abcam antibody was used: AlexaFluor 488 anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/2000
Additional data
Additional Notes
SERCA1 in C2C12 myoblasts were probed with IIH11 antibody. Circular morphology in the center of the cell correspond to DAPI counterstain (not shown). Network of reticular structure in perinuclear region indicates a presence of SERCA1 at ER.
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投稿 Sep 25 2012