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Anti-SERCA1 ATPase 抗体 [IIH11] (ab2818)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [IIH11] to SERCA1 ATPase
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

製品の概要

  • 製品名

    Anti-SERCA1 ATPase antibody [IIH11]
    SERCA1 ATPase 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [IIH11] to SERCA1 ATPase

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • ポジティブ･コントロール

    • WB: Mouse and rat skeletal muscle tissue lysate. IHC-P: Mouse and rat skeletal muscle tissue.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.466 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
IHC-P Use a concentration of 0.177 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.

  • 組織特異性

    Skeletal muscle, fast twitch muscle (type II) fibers.

  • 関連疾患

    Brody myopathy

  • 配列類似性

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.

  • 発生段階

    Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle.

  • 細胞内局在

    Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • fast twitch skeletal muscle isoform antibody
      • AT2A1_HUMAN antibody
      • ATP2A antibody
      • ATP2A1 antibody
      • ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody
      • ATPase Ca++ transporting fast twitch 1 antibody
      • ATPase, Ca(2+)-transporting fast twitch 1 antibody
      • Calcium pump 1 antibody
      • Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody
      • Calcium-transporting ATPase sarcoplasmic reticulum type antibody
      • EC 3.6.3.8 antibody
      • Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody
      • Fast skeletal muscle SR calcium ATPase antibody
      • OTTHUMP00000162561 antibody
      • OTTHUMP00000162562 antibody
      • Sarcoendoplasmic reticulum calcium ATPase antibody
      • Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody
      • Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody
      • SERCA 1 antibody
      • SERCA1 antibody
      • SERCA1 truncated isoform, included antibody
      • SR Ca(2+) ATPase 1 antibody
      • SR Ca(2+)-ATPase 1 antibody
      see all

    画像

    • Western blot - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)
      Western blot - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)
      All lanes : Anti-SERCA1 ATPase antibody [IIH11] (ab2818) at 1/1000 dilution

      Lane 1 : Mouse skeletal muscle tissue lysate
      Lane 2 : Rat skeletal muscle tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Predicted band size: 110 kDa
      Observed band size: 110 kDa


      Exposure time: 48 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)

      Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling SERCA1 ATPase with ab2818 at 0.177µg/ml, followed by ready to use secondary. Cytoplasmic staining on rat skeletal muscle tissue is observed. The section was incubated with ab2818 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)

      Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling SERCA1 ATPase with ab2818 at 0.117µg/ml, followed by ready to use secondary. Cytoplasmic staining on mouse skeletal muscle tissue is observed. The section was incubated with ab2818 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 mins.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (10)

    ab2818 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2818 は 10 報の論文で使用されています。

    • Hofhuis J  et al. Dysferlin mediates membrane tubulation and links T-tubule biogenesis to muscular dystrophy. J Cell Sci 130:841-852 (2017). PubMed: 28104817
    • Murphy S  et al. Concurrent Label-Free Mass Spectrometric Analysis of Dystrophin Isoform Dp427 and the Myofibrosis Marker Collagen in Crude Extracts from mdx-4cv Skeletal Muscles. Proteomes 3:298-327 (2015). PubMed: 28248273
    • Murphy S  et al. Simultaneous Pathoproteomic Evaluation of the Dystrophin-Glycoprotein Complex and Secondary Changes in the mdx-4cv Mouse Model of Duchenne Muscular Dystrophy. Biology (Basel) 4:397-423 (2015). PubMed: 26067837
    • Funai K  et al. Muscle lipogenesis balances insulin sensitivity and strength through calcium signaling. J Clin Invest 123:1229-40 (2013). PubMed: 23376793
    • Hernández A  et al. Dietary nitrate increases tetanic [Ca2+]i and contractile force in mouse fast-twitch muscle. J Physiol 590:3575-83 (2012). PubMed: 22687611
    • Abcejo AJ  et al. Brain-derived neurotrophic factor enhances calcium regulatory mechanisms in human airway smooth muscle. PLoS One 7:e44343 (2012). PubMed: 22952960
    • Yamada T  et al. Impaired mitochondrial respiration and decreased fatigue resistance followed by severe muscle weakness in skeletal muscle of mitochondrial DNA mutator mice. J Physiol 590:6187-97 (2012). PubMed: 22988144
    • Harris RL  et al. Tail muscle parvalbumin content is decreased in chronic sacral spinal cord injured rats with spasticity. Exp Physiol 96:1311-20 (2011). PubMed: 21930674
    • Bruton JD  et al. Increased fatigue resistance linked to Ca2+-stimulated mitochondrial biogenesis in muscle fibres of cold-acclimated mice. J Physiol 588:4275-88 (2010). PubMed: 20837639
    • Eizema K  et al. Differential expression of calcineurin and SR Ca2+ handling proteins in equine muscle fibers during early postnatal growth. J Histochem Cytochem 55:247-54 (2007). PubMed: 17101725

    レビューと Q&A

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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SERCA1 ATPase antibody [IIH11]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (C2C12)
    Specification
    C2C12
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% Triton X-100
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 25 2012

    Western blot abreview for Anti-SERCA1 ATPase antibody [IIH11]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (C2C12)
    Loading amount
    100 µg
    Specification
    C2C12
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 09 2012

    Immunoprecipitation abreview for Anti-SERCA1 ATPase antibody [IIH11]

     Excellent
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - whole cell (C2C12)
    Total protein in input
    500 µg
    Specification
    C2C12
    Immuno-precipitation step
    Protein A
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 08 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com