Anti-SERCA1 ATPase 抗体 [IIH11] (ab2818)
![Western blot - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)](https://www.abcam.co.jp/ps/products/2/ab2818/Images/ab2818-367710-AntiSERCA1-ATPase-antibody-IIH11-western-blot-skeletal-muscle-mouse-rat.jpg "Western blot - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [IIH11] to SERCA1 ATPase
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
製品の概要
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製品名
Anti-SERCA1 ATPase antibody [IIH11]
SERCA1 ATPase 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [IIH11] to SERCA1 ATPase -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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ポジティブ･コントロール
- WB: Mouse and rat skeletal muscle tissue lysate. IHC-P: Mouse and rat skeletal muscle tissue.
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特記事項
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
IIH11 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
アプリケーション
Our Abpromise guarantee covers the use of ab2818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.466 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). | |
| IHC-P | Use a concentration of 0.177 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. -
組織特異性
Skeletal muscle, fast twitch muscle (type II) fibers. -
関連疾患
Brody myopathy -
配列類似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. -
発生段階
Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle. -
細胞内局在
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 11937 Mouse
- Entrez Gene: 116601 Rat
- SwissProt: Q8R429 Mouse
- SwissProt: Q64578 Rat
- Unigene: 35134 Mouse
- Unigene: 217139 Rat
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別名
- fast twitch skeletal muscle isoform antibody
- AT2A1_HUMAN antibody
- ATP2A antibody
see all
画像
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Western blot - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)All lanes : Anti-SERCA1 ATPase antibody [IIH11] (ab2818) at 1/1000 dilution
Lane 1 : Mouse skeletal muscle tissue lysate
Lane 2 : Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 48 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)](https://www.abcam.co.jp/ps/products/2/ab2818/Images/ab2818-367709-AntiSERCA1-ATPase-antibody-IIH11-immunohistochemistry-skeletal-muscle-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling SERCA1 ATPase with ab2818 at 0.177µg/ml, followed by ready to use secondary. Cytoplasmic staining on rat skeletal muscle tissue is observed. The section was incubated with ab2818 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)](https://www.abcam.co.jp/ps/products/2/ab2818/Images/ab2818-367707-AntiSERCA1-ATPase-antibody-IIH11-immunohistochemistry-skeletal-muscle-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA1 ATPase antibody [IIH11] (ab2818)Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling SERCA1 ATPase with ab2818 at 0.117µg/ml, followed by ready to use secondary. Cytoplasmic staining on mouse skeletal muscle tissue is observed. The section was incubated with ab2818 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 mins.
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![Anti-SERCA1 ATPase antibody [IIH11] (ab2818)](https://www.abcam.co.jp/ps/products/2/ab2818/Images/ab2818-10-benefits-of-recombinant-antibodies.png)
Anti-SERCA1 ATPase antibody [IIH11] (ab2818)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (10)
ab2818 は 10 報の論文で使用されています。
- Hofhuis J et al. Dysferlin mediates membrane tubulation and links T-tubule biogenesis to muscular dystrophy. J Cell Sci 130:841-852 (2017). PubMed: 28104817
- Murphy S et al. Concurrent Label-Free Mass Spectrometric Analysis of Dystrophin Isoform Dp427 and the Myofibrosis Marker Collagen in Crude Extracts from mdx-4cv Skeletal Muscles. Proteomes 3:298-327 (2015). PubMed: 28248273
- Murphy S et al. Simultaneous Pathoproteomic Evaluation of the Dystrophin-Glycoprotein Complex and Secondary Changes in the mdx-4cv Mouse Model of Duchenne Muscular Dystrophy. Biology (Basel) 4:397-423 (2015). PubMed: 26067837
- Funai K et al. Muscle lipogenesis balances insulin sensitivity and strength through calcium signaling. J Clin Invest 123:1229-40 (2013). PubMed: 23376793
- Hernández A et al. Dietary nitrate increases tetanic [Ca2+]i and contractile force in mouse fast-twitch muscle. J Physiol 590:3575-83 (2012). PubMed: 22687611
- Abcejo AJ et al. Brain-derived neurotrophic factor enhances calcium regulatory mechanisms in human airway smooth muscle. PLoS One 7:e44343 (2012). PubMed: 22952960
- Yamada T et al. Impaired mitochondrial respiration and decreased fatigue resistance followed by severe muscle weakness in skeletal muscle of mitochondrial DNA mutator mice. J Physiol 590:6187-97 (2012). PubMed: 22988144
- Harris RL et al. Tail muscle parvalbumin content is decreased in chronic sacral spinal cord injured rats with spasticity. Exp Physiol 96:1311-20 (2011). PubMed: 21930674
- Bruton JD et al. Increased fatigue resistance linked to Ca2+-stimulated mitochondrial biogenesis in muscle fibres of cold-acclimated mice. J Physiol 588:4275-88 (2010). PubMed: 20837639
- Eizema K et al. Differential expression of calcineurin and SR Ca2+ handling proteins in equine muscle fibers during early postnatal growth. J Histochem Cytochem 55:247-54 (2007). PubMed: 17101725
