Anti-Semaphorin 7a 抗体 [MEM-150] (ab8222)
Key features and details
- Mouse monoclonal [MEM-150] to Semaphorin 7a
- Suitable for: Flow Cyt, IP, WB
- Reacts with: Human
- Isotype: IgM
製品の概要
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製品名
Anti-Semaphorin 7a antibody [MEM-150]
Semaphorin 7a 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MEM-150] to Semaphorin 7a -
由来種
Mouse -
特異性
Human CD108 (JMH blood group antigen). -
アプリケーション
適用あり: Flow Cyt, IP, WBmore details -
種交差性
交差種: Human -
免疫原
Tissue/ cell preparation (Human). (T cell line HPB-ALL).
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Proprietary Purification -
特記事項（精製）
Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
MEM-150 -
ミエローマ
unknown -
アイソタイプ
IgM -
軽鎖の種類
unknown -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab8222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | Use a concentration of 4 µg/ml. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
|
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Use under non-reducing conditions. |
ターゲット情報
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機能
Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. -
組織特異性
Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal chord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney. -
配列類似性
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
細胞内局在
Cell membrane. Detected in a punctate pattern on the cell membrane of basal and supra-basal skin keratinocytes. - Information by UniProt
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参照データベース
- Entrez Gene: 8482 Human
- Omim: 607961 Human
- SwissProt: O75326 Human
- Unigene: 24640 Human
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別名
- CD 108 antibody
- CD108 antibody
- CD108 antigen antibody
see all
画像
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![Flow Cytometry - Anti-Semaphorin 7a antibody [MEM-150] (ab8222)](https://www.abcam.co.jp/ps/products/8/ab8222/Images/ab8222-331846-anti-semaphorin-7a-antibody-mem-150-flow-cytometry.jpg)
Flow Cytometry - Anti-Semaphorin 7a antibody [MEM-150] (ab8222)Flow cytometry of human peripheral blood cells with ab8222 at 3 µg/ml
プロトコール
参考文献 (3)
ab8222 は 3 報の論文で使用されています。
- Drukker M et al. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Nat Biotechnol 30:531-42 (2012). Human . PubMed: 22634564
- Angelisova P et al. Characterization of the human leukocyte GPI-anchored glycoprotein CDw108 and its relation to other similar molecules. Immunobiology 200:234-45 (1999). PubMed: 10416131
- Mudad R et al. Evidence that CDw108 membrane protein bears the JMH blood group antigen. Transfusion 35:566-70 (1995). PubMed: 7631388
