Anti-Semaphorin 7a 抗体 - BSA and Azide free (Detector) (ab272369)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal to Semaphorin 7a - BSA and Azide free (Detector)
- Suitable for: Sandwich ELISA
- Reacts with: Human
製品の概要
-
製品名
Anti-Semaphorin 7a antibody - BSA and Azide free (Detector)
Semaphorin 7a 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal to Semaphorin 7a - BSA and Azide free (Detector) -
由来種
Rabbit -
アプリケーション
適用あり: Sandwich ELISAmore details -
種交差性
交差種: Human -
特記事項
ab272369 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Semaphorin 7A. The recommended pair for sandwich ELISA is:
Capture: ab272368, Human Semaphorin 7A Capture Antibody (unconjugated)
Detector: ab272369, Human Semaphorin 7A Detector Antibody (unconjugated)
The reference range value is 70.3125 - 4500 pg/ml.The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
ポリ/モノ
モノクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Conjugation kits
-
sELISA pair antibody
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795).
|
| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795). |
ターゲット情報
-
機能
Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. -
組織特異性
Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal chord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney. -
配列類似性
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
細胞内局在
Cell membrane. Detected in a punctate pattern on the cell membrane of basal and supra-basal skin keratinocytes. - Information by UniProt
-
参照データベース
- Entrez Gene: 8482 Human
- Omim: 607961 Human
- SwissProt: O75326 Human
- Unigene: 24640 Human
-
別名
- CD 108 antibody
- CD108 antibody
- CD108 antigen antibody
see all
画像
-
Anti-Semaphorin 7a antibody - BSA and Azide free (Detector) (ab272369)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272369 は論文での使用が確認できていません。
