Application
Western blot
Sample
Xenopus laevis Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (Nupage 4-12% Bis-Tris)
Loading amount
15 µg
Treatment
125uM and 250uM SEMA3A morpholino
Specification
brain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 20°C · Diluent: 5% milk in TBST
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Abcam antibody:(not specified yet)
Detection
Detection method
ECL
Negative control
spleen
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 120 kDa Non-specific: 120 kDa
Positive control
brain lysate
Additional data
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Katrin Mooslehner
Verified customer
投稿 Mar 05 2021