Secretory Phospholipase A2 Assay Kit (ab133089)
製品の概要
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製品名
Secretory Phospholipase A2 Assay Kit -
検出方法
Colorimetric -
サンプルの種類
Plasma -
アッセイタイプ
Enzyme activity (quantitative) -
種交差性
交差種: Mammals, Other species -
製品の概要
Secretory Phospholipase A2 Assay Kit (ab133089) provides an accurate and convenient method for measurement of secretory PLA2 (sPLA2) activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s (e.g., bee and cobra venoms, pancreatic, etc.) with the exception of cytosolic PLA2. Upon hydrolysis of the thio ester bond at the sn-2 position by PLA2, free thiols are detected using DTNB (5,5’-dithio-bis-(2-nitrobenzoic acid)).
If you want to detect cytosolic PLA2, we recommend using Cytosolic Phospholipase A2 Assay Kit (ab133090).
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 96 tests 96-Well Plate (Colorimetric Assay) 1 x 96 tests 96-Well Plate Cover 1 unit Bee venom PLA2 Control 1 vial sPLA2 Assay Buffer (10X) 1 vial sPLA2 Diheptanoyl Thio-PC (Substrate) 2 vials sPLA2 DTNB 4 vials -
研究分野
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機能
Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. -
配列類似性
Belongs to the phospholipase A2 family. -
細胞内局在
Membrane. - Information by UniProt
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別名
- EC 3.1.1.4
- GIIC sPLA2
- Group IIA phospholipase A2
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プロトコール
参考文献
This product has been referenced in:
- Tamayose CI et al. Non-Clinical Studies for Evaluation of 8-C-Rhamnosyl Apigenin Purified from Peperomia obtusifolia against Acute Edema. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 28906474) »
- Lewin M et al. Varespladib (LY315920) Appears to Be a Potent, Broad-Spectrum, Inhibitor of Snake Venom Phospholipase A2 and a Possible Pre-Referral Treatment for Envenomation. Toxins (Basel) 8:N/A (2016). Read more (PubMed: 27571102) »