Phospholipase A2 Assay Kit (ab133089)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Plasma
製品の概要
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製品名
Phospholipase A2 Assay Kit
Phospholipase A2 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Plasma -
アッセイタイプ
Enzyme activity (quantitative) -
種交差性
交差種: Mammals, Other species -
製品の概要
Phospholipase A2 Assay Kit (ab133089) provides an accurate and convenient method for measurement of PLA2 activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s (e.g., bee and cobra venoms, pancreatic, etc.) with the exception of cytosolic PLA2. Upon hydrolysis of the thio ester bond at the sn-2 position by PLA2, free thiols are detected using DTNB (5,5’-dithio-bis-(2-nitrobenzoic acid)).
If you want to detect cytosolic PLA2, we recommend using Cytosolic Phospholipase A2 Assay Kit (ab133090).
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特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 96 tests 96-Well Plate (Colorimetric Assay) 1 x 96 tests 96-Well Plate Cover 1 unit Bee venom PLA2 Control 1 vial PLA2 Assay Buffer (10X) 1 vial PLA2 Diheptanoyl Thio-PC (Substrate) 2 vials PLA2 DTNB 4 vials -
研究分野
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機能
Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. -
配列類似性
Belongs to the phospholipase A2 family. -
細胞内局在
Membrane. - Information by UniProt
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別名
- EC 3.1.1.4
- GIIC sPLA2
- Group IIA phospholipase A2
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab133089 は 3 報の論文で使用されています。
- Koundouros N et al. Metabolic Fingerprinting Links Oncogenic PIK3CA with Enhanced Arachidonic Acid-Derived Eicosanoids. Cell 181:1596-1611.e27 (2020). PubMed: 32559461
- Tamayose CI et al. Non-Clinical Studies for Evaluation of 8-C-Rhamnosyl Apigenin Purified from Peperomia obtusifolia against Acute Edema. Int J Mol Sci 18:N/A (2017). PubMed: 28906474
- Lewin M et al. Varespladib (LY315920) Appears to Be a Potent, Broad-Spectrum, Inhibitor of Snake Venom Phospholipase A2 and a Possible Pre-Referral Treatment for Envenomation. Toxins (Basel) 8:N/A (2016). PubMed: 27571102