Anti-SEC61A 抗体 (ab1327)
Key features and details
- Goat polyclonal to SEC61A
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SEC61A antibody
SEC61A 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to SEC61A -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow, Dog, Pig
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免疫原
Synthetic peptide:
C-KEQSEVGSMGALLF
, corresponding to C terminal amino acids 463-476 of Human SEC61A(Peptide available as ab22804.) -
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab1327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa). 1 hour primary incubation is recommended for this product. |
|
| IHC-P | Use a concentration of 3.75 µg/ml. |
ターゲット情報
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機能
Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins. -
配列類似性
Belongs to the secY/SEC61-alpha family. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 505064 Cow
- Entrez Gene: 404006 Dog
- Entrez Gene: 29927 Human
- Entrez Gene: 53421 Mouse
- Omim: 609213 Human
- SwissProt: Q5EA68 Cow
- SwissProt: P38377 Dog
- SwissProt: P61619 Human
see all -
別名
- chunp6898 antibody
- fb62c11 antibody
- HSEC61 antibody
see all
画像
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Western blot - Anti-SEC61A antibody (ab1327)Anti-SEC61A antibody (ab1327) at 1 µg/ml + Human cerebellum lysate in RIPA buffer at 35 µg
Predicted band size: 52 kDa
プロトコール
データシートおよび資料
参考文献 (1)
ab1327 は 1 報の論文で使用されています。
- Lloyd DJ et al. A point mutation in Sec61alpha1 leads to diabetes and hepatosteatosis in mice. Diabetes 59:460-70 (2010). ICC/IF ; Mouse . PubMed: 19934005
