Anti-SEC61A 抗体 (ab1327)

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Key features and details

  • Goat polyclonal to SEC61A
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-SEC61A antibody
    SEC61A 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to SEC61A

  • 由来種

    Goat

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Cow, Dog, Pig

  • 免疫原

    Synthetic peptide:

    C-KEQSEVGSMGALLF

    , corresponding to C terminal amino acids 463-476 of Human SEC61A(Peptide available as ab22804.)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Human cerebellum and mouse brain lysate.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.05% Tris, 0.5% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).

1 hour primary incubation is recommended for this product.
IHC-P Use a concentration of 3.75 µg/ml.

ターゲット情報

  • 機能

    Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins.

  • 配列類似性

    Belongs to the secY/SEC61-alpha family.

  • 細胞内局在

    Endoplasmic reticulum membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • chunp6898 antibody
    • fb62c11 antibody
    • HSEC61 antibody
    • protein transport protein SEC61 alpha subunit antibody
    • protein transport protein Sec61 subunit alpha antibody
    • Protein transport protein Sec61 subunit alpha isoform 1 antibody
    • S61A1_HUMAN antibody
    • SEC 61 antibody
    • SEC 61A antibody
    • Sec61 alpha 1 antibody
    • Sec61 alpha 1 subunit antibody
    • Sec61 alpha 1 subunit (S. cerevisiae) antibody
    • Sec61 alpha-1 antibody
    • SEC61 antibody
    • Sec61 homolog antibody
    • SEC61A1 antibody
    • sec61aa antibody
    see all

画像

  • Western blot - Anti-SEC61A antibody (ab1327)
    Western blot - Anti-SEC61A antibody (ab1327)
    Anti-SEC61A antibody (ab1327) at 1 µg/ml + Human cerebellum lysate in RIPA buffer at 35 µg

    Predicted band size: 52 kDa

参考文献 (1)

ab1327 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1327 は 1 報の論文で使用されています。

  • Lloyd DJ  et al. A point mutation in Sec61alpha1 leads to diabetes and hepatosteatosis in mice. Diabetes 59:460-70 (2010). ICC/IF ; Mouse . PubMed: 19934005

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