リコンビナントRabMAb

Anti-SEC23B + SEC23A 抗体 [EPR22723-102] - BSA and Azide free (ab270757)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] - BSA and Azide free (ab270757)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] - BSA and Azide free (ab270757)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] - BSA and Azide free (ab270757)
  • Anti-SEC23B + SEC23A antibody [EPR22723-102] - BSA and Azide free (ab270757)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22723-102] to SEC23B + SEC23A - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-SEC23B + SEC23A antibody [EPR22723-102] - BSA and Azide free

  • 製品の詳細

    Rabbit monoclonal [EPR22723-102] to SEC23B + SEC23A - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Purified LIF/His-tagged SEC23B recombinant protein, His-tagged SEC23A recombinant protein;human liver lysate; moues liver lysate; MEF, HeLa, Raji, C6, RAW 264.7, PC-12, and NIH/3T3 whole cell lysates. IHC-P: Human cerebrum tissue; mouse liver tissue, rat kidney tissue.

  • 特記事項

    ab270757 is the carrier-free version of ab270750. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] - BSA and Azide free (ab270757)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] - BSA and Azide free (ab270757)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive cytoplasmic staining on rat kidney (PMID: 22745161).

    The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (270750).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] - BSA and Azide free (ab270757)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] - BSA and Azide free (ab270757)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive cytoplasmic staining on mouse liver (PMID: 22745161).

    The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (270750).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] - BSA and Azide free (ab270757)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] - BSA and Azide free (ab270757)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive cytoplasmic staining on human cerebrum (PMID: 22745161) is observed.

    The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (270750).

  • Anti-SEC23B + SEC23A antibody [EPR22723-102] - BSA and Azide free (ab270757)
    Anti-SEC23B + SEC23A antibody [EPR22723-102] - BSA and Azide free (ab270757)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab270757 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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