Anti-SEC23B + SEC23A 抗体 [EPR22723-102] (ab270750)

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22723-102] to SEC23B + SEC23A
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human, Recombinant fragment

製品名

Anti-SEC23B + SEC23A antibody [EPR22723-102]
製品の詳細

Rabbit monoclonal [EPR22723-102] to SEC23B + SEC23A
由来種

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Purified LIF/His-tagged SEC23B recombinant protein, His-tagged SEC23A recombinant protein; human liver lysate; mouse liver lysate; MEF, HeLa, Raji, C6, RAW 264.7, PC-12, and NIH/3T3 whole cell lysates. IHC-P: Human cerebrum tissue; mouse liver tissue, rat kidney tissue.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR22723-102
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-SEC23B + SEC23A antibody [EPR22723-102] - BSA and Azide free (ab270757)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Related Products
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション	Species
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

アプリケーション	Abreviews	特記事項
WB		1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
IHC-P		1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

特記事項
WB 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

細胞内局在

SEC23B: Golgi apparatus membrane. Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. SEC23: Smooth endoplasmic reticulum membrane. Golgi apparatus membrane. In the ribosome-free transitional face of the ER and associated vesicles.
参照データベース
- Entrez Gene: 10483 Human
- Entrez Gene: 10484 Human
- Entrez Gene: 20334 Mouse
- Entrez Gene: 27054 Mouse
- Entrez Gene: 362226 Rat
- Entrez Gene: 58817 Rat
- Omim: 610511 Human
- Omim: 610512 Human
- SwissProt: Q15436 Human
- SwissProt: Q15437 Human
- SwissProt: Q01405 Mouse
- SwissProt: Q9D662 Mouse
- Unigene: 272927 Human
- Unigene: 369373 Human
- Unigene: 248492 Mouse
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive cytoplasmic staining on rat kidney (PMID: 22745161).

The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

All lanes : Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750) at 1/1000 dilution

Lane 1 : MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Raji (human burkitts lymphoma b lymphocyte) whole cell lysate
Lane 4 : Mouse liver tissue lysate
Lane 5 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 6 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 7 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 8 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 8 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 mg/ml

Predicted band size: 86 kDa
Observed band size: 86 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 22745161).

Exposure time: Lanes 1-4: 26 seconds;Lanes 5-8: 10 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive cytoplasmic staining on mouse liver (PMID: 22745161).

The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750) at 1/1000 dilution + Human liver tissue lysate at 20 µg

Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 86 kDa
Observed band size: 86 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 22745161).

Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive cytoplasmic staining on human cerebrum (PMID: 22745161) is observed.

The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

All lanes : Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750) at 1/1000 dilution

Lane 1 : Purified LIF/His-tagged SEC23B recombinant protein (aa127-392), 10 ng
Lane 2 : His-tagged SEC23A recombinant protein (aa127-392)

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 86 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Both the recombinant proteins were made in house.

SEC23B recombinant protein was expressed from a mammalian - HEK-293 expression system. The LIF tag is about 40 kDa.

SEC23A recombinant protein was from E.coil extracts.

Exposure time: 3 seconds.
Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet

ab270750 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab270750 は論文での使用が確認できていません。

レビューと Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Anti-SEC23B + SEC23A 抗体 [EPR22723-102] (ab270750)

Key features and details

製品の概要

製品名

製品の詳細

由来種

Tested Applications & Species

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

細胞内局在

参照データベース

画像

プロトコール

データシートおよび資料

Certificate of Compliance

参考文献 (0)

レビューと Q&A