Application
Western blot
Sample
Caenorhabditis elegans Tissue lysate - other (Isolated Mitochondria)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
60 µg
Specification
Isolated Mitochondria
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 2% Milk in TBS-T
Dilution
1/10000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: anti-Mouse HRP Ab
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: - kDa Non-specific: 32 kDa
Additional data
Abcam response
We would like to thank the reviewer for submitting this Abreview for this product.
As the product had not yet been tested in this application on this tissue type we were unsure what the result might be, and we appreciate the time and effort that the reviewer has put in to testing this product in this application.
We encourage all our customers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial Testing Program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Amber Knapp-Wilson
Verified customer
投稿 Nov 26 2018