Application
Western blot
Sample
Mouse Cell lysate - other (AML12 hepatocytes, mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (18)
Loading amount
25 µg
Specification
AML12 hepatocytes, mitochondrial fraction
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
1 hour(s) and 45 minute(s) · Temperature: 23°C · Diluent: PBS 1% BSA
Concentration
0.1 µg/ml
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used:
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
SuperSignal Pico ECL
Negative control
cytoplasmic fraction
Exposure
10 second(s)
Bands
Specific: 74 kDa
Additional data
Additional Notes
Lane 1: Thermo Scientific PageRuler Prestained Protein Ladder
Lanes 2-3: Fly mitochondrial fraction
Lane 4: Fly cytoplasmic fraction
Lane 5: Robin mitochondrial fraction
Lane 6: Robin cytoplasmic fraction
Lane 7: Mouse (AML12) mitochondrial fraction
Lane 8: Mouse (AML12) cytoplasmic fraction
Lane 9: Bio-Rad Precision Plus Protein WesternC Standards
Mitochondrial and cytoplasmic fractions were obtained from whole flies with Abcam Mitochondrial Isolation Kit for Tissue (ab110168) and from robin fibroblast and mouse hepatocyte cell lines with Abcam Mitochondrial Isolation Kit for Cells (ab110170).
This antibody works well with both samples run on reducing and native gels.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 25 2017