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遺伝子ノックアウト細胞株 検証済

Anti-SDHA 抗体 [2E3GC12FB2AE2] (ab14715)

  • Datasheet
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Reviews (24)Q&A (3)References (356)

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Western blot - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
  • Western blot - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
  • Immunocytochemistry - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
  • Immunohistochemistry (Frozen sections) - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
  • Immunocytochemistry - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
  • Flow Cytometry - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)

Key features and details

  • Mouse monoclonal [2E3GC12FB2AE2] to SDHA
  • Suitable for: IHC-Fr, Flow Cyt, WB, ICC, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 HRP

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Anti-MTCO1 antibody [1D6E1A8] (ab14705)

関連製品

製品の概要

  • 製品名

    Anti-SDHA antibody [2E3GC12FB2AE2]
    SDHA 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [2E3GC12FB2AE2] to SDHA
  • 由来種

    Mouse
  • アプリケーション

    適用あり: IHC-Fr, Flow Cyt, WB, ICC, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Cow, Human
    交差が予測される動物種: Dog
  • 免疫原

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • ポジティブ・コントロール

    • WB: HEK-293, MCF7 amd HEepG2 whole cell lysates; Mitochondria isolated from human heart, cow heart, rat heart, mouse heart and HepG2 cells. Flow cyt: HL-60 cells. IHC-P: Human testis and skeletal muscle tissue. ICC: Cultured human embryonic lung-derived fibroblasts (strain MRC5).
  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C.
  • バッファー

    pH: 7.5
    Preservative: 0.02% Sodium azide
    Constituent: HEPES buffered saline
  • Concentration information loading...
  • 精製度

    IgG fraction
  • 特記事項(精製)

    Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • ポリ/モノ

    モノクローナル
  • クローン名

    2E3GC12FB2AE2
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    kappa
  • 研究分野

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex II
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-SDHA antibody [2E3GC12FB2AE2] (ab154473)
    • Alexa Fluor® 594 Anti-SDHA antibody [2E3GC12FB2AE2] (ab170172)
    • HRP Anti-SDHA antibody [2E3GC12FB2AE2] (ab198493)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human SDHA protein (Tagged) (ab226268)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab14715の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt (1)
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB (17)
Use a concentration of 0.1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ICC
Use at an assay dependent concentration.
IHC-P (4)
Use at an assay dependent concentration.
特記事項
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
Use a concentration of 0.1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
  • パスウェイ

    Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.
  • 関連疾患

    Defects in SDHA are a cause of mitochondrial complex II deficiency (MT-C2D) [MIM:252011]. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations. Clinical features include psychomotor regression in infants, poor growth with lack of speech development, severe spastic quadriplegia, dystonia, progressive leukoencephalopathy, muscle weakness, exercise intolerance, cardiomyopathy. Some patients manifest Leigh syndrome or Kearns-Sayre syndrome.
    Defects in SDHA are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
    Defects in SDHA are the cause of cardiomyopathy dilated type 1GG (CMD1GG) [MIM:613642]. CMD1GG is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • 配列類似性

    Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.
  • 細胞内局在

    Mitochondrion inner membrane.
  • Target information above from: UniProt accession P31040 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 281480 Cow
    • Entrez Gene: 478634 Dog
    • Entrez Gene: 6389 Human
    • Entrez Gene: 66945 Mouse
    • Entrez Gene: 157074 Rat
    • Omim: 600857 Human
    • SwissProt: P31039 Cow
    • SwissProt: P31040 Human
    • SwissProt: Q8K2B3 Mouse
    • SwissProt: Q920L2 Rat
    • Unigene: 440475 Human
    • Unigene: 158231 Mouse
    • Unigene: 101725 Rat
    see all
  • 別名

    • CMD1GG antibody
    • DHSA_HUMAN antibody
    • Flavoprotein subunit of complex II antibody
    • Fp antibody
    • PGL5 antibody
    • SDH 2 antibody
    • SDH1 antibody
    • SDH2 antibody
    • SDHA antibody
    • SDHF antibody
    • Succinate dehydrogenase [ubiquinone] flavoprotein subunit antibody
    • Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial antibody
    • Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial antibody
    • Succinate dehydrogenase complex flavoprotein subunit A antibody
    • Succinate dehydrogenase complex flavoprotein subunit antibody
    • Succinate dehydrogenase complex flavoprotein subunit precursor antibody
    • Succinate dehydrogenase complex subunit A antibody
    • Succinate dehydrogenase complex subunit A flavoprotein (Fp) antibody
    • Succinate dehydrogenase complex subunit A flavoprotein antibody
    see all

画像

  • Western blot - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    Western blot - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    All lanes : HRP Anti-SDHA antibody [2E3GC12FB2AE2] (ab198493) at 1/5000 dilution

    Lane 1 : Wild-type HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : SDHA knockout HEK-293 whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 70 kDa



    This data was developed using the same antibody clone in a different format (HRP conjugated) (ab198493).

    Lanes 1 - 4: Merged signal (red and green). Green - ab198493 observed at 72 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab198493 was shown to recognize in wild-type HEK-293 cells as signal was lost at the expected MW in SDHA knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SDHA knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab198493 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)Image from Phillips J et al., Sci Rep. 2016 May 16;6:26013. Fig 4a doi: 10.1038/srep26013. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

    250 μm-thick formalin-fixed human cerebellum section were passively cleared using PACT and immunofluorescently labelled to identify mitochondrial mass (porin (ab14734, 1/100) and SDHA (ab14715, 1/100), 647 nm) and complex I subunits within the mitochondrial respiratory chain (NDUFB8 (ab110242, 1/100) and NDUFA13 (ab110240, 1/100); 546 nm) in conjunction with a neuronal marker (NF-H; 488 nm) in control 1. Scale: 100 μm.

  • Western blot - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    Western blot - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    All lanes : Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 4 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
    Lane 5 : Isolated mitochondria from HepG2 (human liver hepatocellular carcinoma cell line) at 20 µg

    Predicted band size: 70 kDa
    Observed band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    ab14715 (2µg/ml) staining SDHA in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and mitochondrial staining within the seminal vesicles.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunocytochemistry - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    Immunocytochemistry - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)

    Mitochondrial localization of complex II visualized by immunocytochemistry using ab14715. Cultured human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab14715 (0.2 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody (2 µg/ml).

  • Immunohistochemistry (Frozen sections) - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    Immunohistochemistry (Frozen sections) - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)

    Human skeletal muscle immunohistochemistry using ab14715. Fixed frozen tissue sections from a patient with a single large deletion of the mtDNA were used. All muscle fibers exhibit complex II immunoreactivity, consistent with the nuclear DNA-encoded expression pattern of this and all other subunits of complex II.

  • Immunocytochemistry - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    Immunocytochemistry - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)

    ICC/IF image of ab14715 stained human HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 0.1% PBS-tween diluted 1%BSA (OR 10% goat serum OR 0.3M glycine) for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14715, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.

  • Flow Cytometry - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
    Flow Cytometry - Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)

    HL-60 (human promyelocytic leukemia cell line) cells were stained with 1 µg/mL ab14715 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

プロトコール

  • Mouse on Mouse staining protocol

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (356)

ab14715 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab14715 は 356 報の論文で使用されています。

  • Niklasson CU  et al. Hypoxia inducible factor-2a importance for migration, proliferation, and self-renewal of trunk neural crest cells. Dev Dyn 250:191-236 (2021). PubMed: 32940375
  • Aleo SJ  et al. Drug repositioning as a therapeutic strategy for neurodegenerations associated with OPA1 mutations. Hum Mol Genet 29:3631-3645 (2021). PubMed: 33231680
  • Cirotti C  et al. Redox activation of ATM enhances GSNOR translation to sustain mitophagy and tolerance to oxidative stress. EMBO Rep 22:e50500 (2021). PubMed: 33245190
  • Iwasaki H  et al. MicroRNA-494-3p inhibits formation of fast oxidative muscle fibres by targeting E1A-binding protein p300 in human-induced pluripotent stem cells. Sci Rep 11:1161 (2021). PubMed: 33441918
  • Zehavi Y  et al. A novel de novo heterozygous pathogenic variant in the SDHA gene results in childhood onset bilateral optic atrophy and cognitive impairment. Metab Brain Dis 36:581-588 (2021). PubMed: 33471299
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 27 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SDHA antibody [2E3GC12FB2AE2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 12 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SDHA antibody [2E3GC12FB2AE2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 12 2020

Western blot abreview for Anti-SDHA antibody [2E3GC12FB2AE2]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Caenorhabditis elegans Tissue lysate - other (Isolated Mitochondria)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
60 µg
Specification
Isolated Mitochondria
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MISS. Amber Knapp-Wilson

Verified customer

投稿 Nov 26 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SDHA antibody [2E3GC12FB2AE2]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA ph 9
Permeabilization
No
Specification
brain tissue
Blocking step
Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Herr Dr. Markus Kipp

Verified customer

投稿 Aug 22 2018

Western blot abreview for Anti-SDHA antibody [2E3GC12FB2AE2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (SH-SY5Y)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
SH-SY5Y
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 24 2018

Western blot abreview for Anti-SDHA antibody [2E3GC12FB2AE2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
American Robin (Turdis migratorius) Cell lysate - other (fibroblasts, mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (18%)
Loading amount
25 µg
Specification
fibroblasts, mitochondrial fraction
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 12 2017

Western blot abreview for Anti-SDHA antibody [2E3GC12FB2AE2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - other (AML12 hepatocytes, mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (18)
Loading amount
25 µg
Specification
AML12 hepatocytes, mitochondrial fraction
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Aug 25 2017

Western blot abreview for Anti-SDHA antibody [2E3GC12FB2AE2]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - other (Mouse liver (A) and muscle (B) mitochondria)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (3-12%)
Loading amount
10 µg
Specification
Mouse liver (A) and muscle (B) mitochondria
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Jul 29 2015

Western blot abreview for Anti-SDHA antibody [2E3GC12FB2AE2]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (14% 37.5:1)
Sample
Cow Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
3% Casein in PBS with 0.1% Tween-20 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

投稿 Feb 05 2015

Question

Dear Abcam, I am a current PhD student at Imperial College London working on a project that requires the identification of epitopes bound by antibodies that are ordered. Currently I am considering making a purchase on the SDHA antibody of complex II, described here: https://www.abcam.com/SDHA-antibody-2E3-ab14715.html Unfortunately, details regarding the specific amino acid residues bound by the antibody are not listed or described anywhere that can be found. I was hoping that someone from the company might be able to provide me with this information. Hope to receive a reply at your very earliest convenience. Kind regards,

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Abcam community

Verified customer

Asked on Aug 31 2011

Answer

Thank you for contacting us. We do not know the epitopes to which this mAb binds. The immunogen was a full-length endogenous protein, of bovine origin; this antibody detects Hu, Ms, Rat, Ce, Cow, Dog SDHA protein and its specificity is fully guaranteed. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Abcam Scientific Support

Answered on Aug 31 2011

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