Anti-SDF1 抗体 (ab25117)
Key features and details
- Rabbit polyclonal to SDF1
- Suitable for: IP, IHC-Fr, IHC-P, IHC-FoFr, Flow Cyt, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SDF1 antibody
SDF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SDF1 -
由来種
Rabbit -
アプリケーション
適用あり: IP, IHC-Fr, IHC-P, IHC-FoFr, Flow Cyt, WBmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Human -
免疫原
Recombinant fragment corresponding to Mouse SDF1 aa 20-89.
Database link: P40224 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.50
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25117の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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IHC-Fr | (2) |
Use at an assay dependent concentration.
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IHC-P | (4) |
Use at an assay dependent concentration. PubMed: 21224081
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 25086214
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Flow Cyt |
Use at an assay dependent concentration. PubMed: 20871610
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (3) |
1/1000. Predicted molecular weight: 11 kDa.
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特記事項 |
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IP
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. PubMed: 21224081 |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 25086214 |
Flow Cyt
Use at an assay dependent concentration. PubMed: 20871610 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Predicted molecular weight: 11 kDa. |
ターゲット情報
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機能
Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. -
組織特異性
Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
発生段階
Isoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney. -
翻訳後修飾
Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 6387 Human
- Entrez Gene: 20315 Mouse
- Entrez Gene: 24772 Rat
- Omim: 600835 Human
- SwissProt: P48061 Human
- SwissProt: P40224 Mouse
- Unigene: 522891 Human
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別名
- 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody
- AI174028 antibody
- C-X-C motif chemokine 12 antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (57)
ab25117 は 57 報の論文で使用されています。
- Zhao Q et al. Visfatin inhibits colon cancer cell apoptosis and decreases chemosensitivity to 5‑FU by promoting the SDF‑1/CXCR4/Akt axis. Int J Oncol 60:N/A (2022). PubMed: 35506454
- Chen Q et al. The Role of TMEM16A/ERK/NK-1 Signaling in Dorsal Root Ganglia Neurons in the Development of Neuropathic Pain Induced by Spared Nerve Injury (SNI). Mol Neurobiol 58:5772-5789 (2021). PubMed: 34406600
- Sommer K et al. Local application reduces number of needed EPC for beneficial effects on wound healing compared to systemic treatment in mice. Eur J Trauma Emerg Surg N/A:N/A (2021). PubMed: 33813603
- Wei X et al. Ultrasound-targeted microbubble destruction-mediated Galectin-7-siRNA promotes the homing of bone marrow mesenchymal stem cells to alleviate acute myocardial infarction in rats. Int J Mol Med 47:677-687 (2021). PubMed: 33416139
- Xue Y et al. Role of the HIF-1a/SDF-1/CXCR4 signaling axis in accelerated fracture healing after craniocerebral injury. Mol Med Rep 22:2767-2774 (2020). PubMed: 32945380