Anti-SCGF beta 抗体 (ab9835)

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Key features and details

  • Goat polyclonal to SCGF beta
  • Suitable for: WB, Sandwich ELISA
  • Reacts with: Human
  • Isotype: unknown

製品の概要

  • 製品名

    Anti-SCGF beta antibody
    SCGF beta 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to SCGF beta

  • 由来種

    Goat

  • アプリケーション

    適用あり: WB, Sandwich ELISAmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Highly pure (>98%) recombinant hSCGFb (human Stem Cell Growth Factor beta)

  • ポジティブ･コントロール

    • Recombinant human SCGF beta protein (ab9836) can be used as a positive control in WB.

製品の特性

  • 製品の状態

    Lyophilized:Reconstitute with 200µl of sterile water.

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • バッファー

    pH: 7.40

    No preservative, sterile filtered
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    unknown

  • 軽鎖の種類

    unknown

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.2 µg/ml.

To detect Human SCGF-β by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SCGF-β is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA Use at an assay dependent concentration.

To detect Human SCGF-β by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. 

ターゲット情報

  • 機能

    Stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. Acts synergistically with other cytokines, including IL-3, GCSF, GMCSF and FLT3 ligand. Suppresses SCF-stimulated erythrocyte proliferation.

  • 組織特異性

    Expressed in skeletal tissues including bone marrow, chondrocytes, primary ossification center-associated cells, the perichondrium and periosteum. Lower levels of expression were detected in spleen, thymus, appendix and fetal liver.

  • 配列類似性

    Contains 1 C-type lectin domain.

  • 発生段階

    In the bone marrow, expression is limited to immature neutrophils. Expression was not detected in circulating mature neutrophils.

  • 翻訳後修飾

    O-glycosylated. Probably sulfated on the O-glycans.

  • 細胞内局在

    Cytoplasm. Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C type lectin domain family 11 member A antibody
      • C type lectin superfamily member 3 antibody
      • C-type lectin domain family 11 member A antibody
      • C-type lectin superfamily member 3 antibody
      • CLC11_HUMAN antibody
      • CLEC11A antibody
      • CLECSF3 antibody
      • LSLCL antibody
      • Lymphocyte secreted C type lectin antibody
      • Lymphocyte secreted C-type lectin antibody
      • p47 antibody
      • Stem cell growth factor antibody
      see all

    参考文献 (1)

    ab9835 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9835 は 1 報の論文で使用されています。

    • Hiraoka A Leukemia cell lines require self-secreted stem cell growth factor (SCGF) for their proliferation. Leuk Res 32:1623-5 (2008). Human . PubMed: 18289675

    レビューと Q&A

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    Question


    I want to purchase antibody against SCGF-beta but when look carefully in the product list and surprised that there are two antibody has same specification but different product number.Will you please give the detailed difference between ab9835 and ab110493. Which one better and why?

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    Answer

    Thank you for contacting us. At Abcam we aim to provide our customers with a wide range of products to fit our researchers needs, and this somtimes results in very similar antibodies in our catalog. These two antibodies are both goat polyclonal with the same immunogen, recombinant hSCGFb, have the same purification and the same buffer (PBS). The difference is what applications they are tested for. Both are tested for WB and ELISA, however the antibody ab9835 has been tested for neutralising, while ab110493 has been tested for sandwich ELISA and has a matched pair.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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