Application
Flow Cytometry
Fixation
Formaldehyde
Permeabilization
Yes - BD PERMEABILISATION BUFFER
Sample
Human Cell (HEP G2)
Specification
HEP G2
Gating Strategy
CD24 CELLS
Preparation
Cell harvesting/tissue preparation method: single cell suspension
Sample buffer: 0.1% PBS BSA
Sample buffer: 0.1% PBS BSA
Other product details
Concentration
0.5 µg/cells
Incubation time
40 minute(s) · Temperature: 4°C · Diluent: BD PERMIABILISATION BUFFER
Secondary antibody
Name
Non-Abcam antibody was used: ANTI MOUSE ANTIBODY
Host species: Rabbit
Clonality: Monoclonal
Conjugation: PE-DyLight™ 594
Host species: Rabbit
Clonality: Monoclonal
Conjugation: PE-DyLight™ 594
Dilution
1/1000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Dec 06 2013