Application
Western blot
Sample
Rat Tissue lysate - whole (Rat kidney tissue)
Loading amount
140 µg
Specification
Rat kidney tissue
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST(0.1%)
Secondary antibody
Name
Non-Abcam antibody was used: Anti mouse IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
pierce ECL west pico chemiluminescent su
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 42 kDa Non-specific: 60, 34, 27 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 30 2012