Anti-SCD1 抗体 [CD.E10] (ab19862)

Gracias por contactarnos.

Espero que no te importe que te conteste en castellano. Recientemente hemos establecido la Oficina Virtual Española y podéis contactarnos en este idioma siempre que lo deseéis. Si preferís que os conteste en inglés, por favor, no dudéis en comunicármelo.

Atendiendo al protocolo, es posible que la membrana se haya bloqueado en exceso al haber diluido ambos anticuerpos en buffer de bloqueo. Además, se ha usado leche para diluir el secundario y BSA para el primario, lo cual añade dos pasos extra de bloqueo al ya realizado antes de incubar con anticuerpo.

Yo os sugeriría diluir los anticuerpos en TBST o PBS en lugar de buffer de bloqueo. Y observar si se obtiene un mejor resultado.

Espero que este consejo permita obtener mejores resultados del anticuerpo. En caso de que no sea así, por favor, no dudéis en contactarnos de nuevo para que podamos ayudaros a resolver el problema lo más rápido y eficazmente posible.

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In order to better understand the problem, I would like to send you a questionnaire with some questions about the protocol used, as well as the order details. All this information is crucial to help us understand the possible causes of the problem. Any images are highly appreciated.

Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. If no suggestions can be made to the protocol, and the antibody was purchased within the last 6 months, it is covered by our Abpromise guarantee, and therefore I can offer you a free of charge replacement or a credit note for it.

I look forward to receiving your reply. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us. I am sorry that this antibody is detecting multiple bands in WB on rat samples.
Is the secondary antibody you are using pre-adsorbed against rat? It is possible that the anti-mouse secondary may be detecting the heavy and light chains of endogenous IgGs. The heavy and light chains tend to run around 50 and 25 kDa respectively, but variations in buffer system and sample preparation could raise the observed molecular weights and this may account for the bands around 60 and 27 kDa. I would recommend using a preadsorbed secondary antibody, or a secondary antibody that is designed to only detect native IgGs, such as our new VeriBlot range (ab131368 for example).
I would also recommend loading no more than 50 ug of protein to ensure the maximum specificity possible, especially when using a very sensitive ECL reagent.
I hope this helps to improve your results, if not, I will be happy to offer you a free of charge replacement, credit, or refund as long as you have purchased the antibody in the past six months. Please let me know your original order number and how you would like to proceed and I will be happy to help you further.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab23331.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is notcovered by our Abpromise guarantee. Therefore, I am issuing you a testing discount code for testing this replacement antibody in mouse samples. Please see below for details.

I wish you the best of luck with your research.

===== TESTING DISCOUNT CODE =====

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab23331 in mouse. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouseand include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Thank you for calling Abcam. I have continued the search for the guinea pig sequence for stearyl-CoA desaturase, but I have not been able to locate it. I can find, rat, mouse, sheep, goat, bovine, hamster but not guinea pig. Therefore I cannot do an alignment between the human and g.pig sequences to determine likelihood of cross reactivity. If you are still interested in testing this antibody in guinea pig IHC, then I have put the testing discount details below. If there is anything else I can help with, please let me know. Testing Discount Details: As, ab19832 has not been tested in guinea pig I can offer a discount off a future purchase if you buy ab19832 now, test it in (UNTESTED APP/SPECIES) and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free (PRIMARY ANTIBODY/PROTEIN). If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab19832 in guinea pig. I will then send a discount code. This code must be issued before purchasing ab19832 so please wait for my reply before ordering. 2. Purchase ab19832 either by phone, fax, or online (www.abcam.com). 3. Test it in guinea pig. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab19832 turns out to be unsuitable for guinea pig you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

Thank you for your email. We highly appreciate your cooperation in this. I am sorry we are unable to resolve the problems you have been experiencing. It may be possible that these antibodies are not suitable for Typhoon method with Kpi lysis buffer. The ab19862 was cited in 6 publications as far as we know and in 2 publications PubmedID 19787047 and 18697866 in WB so I might suggest checking the protocol they have used. I have asked our account department to refund the money on the original order 912415. The refund ID is 17800. Please contact our customer service team by quoting this order in case of any question. You can also inform your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice