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RabMAb

Anti-Scavenging Receptor SR-BI 抗体 [EPR20190] (ab217318)

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製品の概要

  • 製品名

    Anti-Scavenging Receptor SR-BI antibody [EPR20190]
    Scavenging Receptor SR-BI 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR20190] to Scavenging Receptor SR-BI

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IF, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment within Human Scavenging Receptor SR-BI aa 1-450. The exact sequence is proprietary.
    Database link: Q8WTV0

  • ポジティブ･コントロール

    • WB: Human fetal liver lysate; Mouse liver, heart, kidney and spleen lysates; Rat liver lysate; U937, LNCaP, PC-3, THP-1, HepG2, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates. IHC-P: Human liver, diffuse large B cell lymphoma and hepatocellular carcinoma tissues; Mouse liver tissue; Rat liver and cerebral cortex tissues. ICC/IF: HepG2 cells. IP: Human fetal liver lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab217318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 82 kDa (predicted molecular weight: 60 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.
IP 1/30.

ターゲット情報

  • 機能

    Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester.

  • 組織特異性

    Widely expressed.

  • 配列類似性

    Belongs to the CD36 family.

  • 翻訳後修飾

    N-glycosylated.

  • 細胞内局在

    Cell membrane. Membrane > caveola. Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CD36 and LIMPII analogous 1 antibody
    • CD36 antibody
    • CD36 Antigen like 1 antibody
    • CD36 antigen-like 1 antibody
    • CD36L1 antibody
    • CLA 1 antibody
    • CLA-1 antibody
    • CLA1 antibody
    • Collagen type I receptor antibody
    • HDLQTL6 antibody
    • MGC138242 antibody
    • SCARB1 antibody
    • Scavebger Receptor Class B Member 1 antibody
    • Scavenger receptor class B member 1 antibody
    • Scavenger Receptor Class B Type 1 antibody
    • SCRB1_HUMAN antibody
    • SR BI antibody
    • SR-BI antibody
    • SRB1 antibody
    • SRBI antibody
    • Thrombospondin receptor like 1 antibody
    • thrombospondin receptor-like 1 antibody
    see all

画像

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
    Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Scavenging Receptor SR-BI knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: Human liver whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab217318 observed at 80 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab217318 was shown to specifically react with Scavenging Receptor SR-BI in wild-type HAP1 cells as signal was lost in Scavenging Receptor SR-BI knockout cells. Wild-type and Scavenging Receptor SR-BI knockout samples were subjected to SDS-PAGE. Ab217318 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
    Immunocytochemistry/ Immunofluorescence - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

    Immunofluorescent analysis of 100% methanol fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Scavenging Receptor SR-BI with ab217318 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on HepG2 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human liver is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
    Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
    All lanes : Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318) at 1/2000 dilution

    Lane 1 : Human fetal liver lysate at 20 µg
    Lane 2 : Mouse liver lysate at 20 µg
    Lane 3 : Rat liver lysate at 20 µg
    Lane 4 : Mouse heart lysate at 10 µg
    Lane 5 : Mouse kidney lysate at 10 µg
    Lane 6 : Mouse spleen lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 60 kDa
    Observed band size: 82 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1-3: 4 seconds; Lane 4-6: 3 minutes.

    Scavenging Receptor SR-BI undergoes N-glycosylation when it is expressed. The molecular weight observed is consistent with what has been described in the literature (PMID: 12016218; PMID: 10821819; PMID: 224442701).

  • Immunoprecipitation - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
    Immunoprecipitation - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

    Scavenging Receptor SR-BI was immunoprecipitated from 0.35 mg of Human fetal liver lysate with ab217318 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab217318 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human fetal liver lysate, 10 μg (Input).

    Lane 2: ab217318 IP in Human fetal liver lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab217318 in Human fetal liver lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
    Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
    All lanes : Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318) at 1/2000 dilution

    Lane 1 : U937 (Human histiocytic lymphoma cell line) whole cell lysate
    Lane 2 : LNCaP (Human prostate cancer cell line) whole cell lysate
    Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 4 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
    Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 60 kDa
    Observed band size: 82 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 30 seconds; Lane 2: 4 seconds; Lane 3: 2 seconds; Lane 4-5: 5 seconds.

     

  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
    Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
    All lanes : Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 60 kDa
    Observed band size: 82 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

    Immunohistochemical analysis of paraffin-embedded human diffuse large B cell lymphoma tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on human diffuse large B cell lymphoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human hepatocellular carcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse liver is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on rat liver is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on rat cerebral cortex blood vessel endothelium is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

参考文献

ab217318 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab217318 は 12 報の論文で使用されています。

  • Yang Z  et al. The fucoidan A3 from the seaweed Ascophyllum nodosum enhances RCT-related genes expression in hyperlipidemic C57BL/6J mice. Int J Biol Macromol 134:759-769 (2019). PubMed: 31100394
  • Tan T  et al. Bioinspired lipoproteins-mediated photothermia remodels tumor stroma to improve cancer cell accessibility of second nanoparticles. Nat Commun 10:3322 (2019). PubMed: 31346166
  • Yin J  et al. The fucoidan from the brown seaweed Ascophyllum nodosum ameliorates atherosclerosis in apolipoprotein E-deficient mice. Food Funct 10:5124-5139 (2019). PubMed: 31364648
  • Zhang G  et al. Prenatal ethanol exposure-induced a low level of foetal blood cholesterol and its mechanism of IGF1-related placental cholesterol transport dysfunction. Toxicology 424:152237 (2019). PubMed: 31226463
  • Chen Y  et al. Differentiation of human adipose derived stem cells into Leydig-like cells with molecular compounds. J Cell Mol Med 23:5956-5969 (2019). PubMed: 31293077
View all Publications for this product

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Western blot abreview for Anti-Scavenging Receptor SR-BI antibody [EPR20190]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Fibroblasts (701 & 1386))
Gel Running Conditions
Reduced Denaturing (Gradient 4-20%)
Loading amount
20 µg
Treatment
siRNA Treatment (ScreenFect Protocol)
Specification
Fibroblasts (701 & 1386)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Mrs. Ilse Kammerhofer

Verified customer

投稿 Feb 12 2020

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