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  1. Link

    scavenging-receptor-sr-bi-antibody-ep1556y-ab52629.pdf

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Microbiology Interspecies Interaction Host Virus Interaction
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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Scavenging Receptor SR-BI 抗体 [EP1556Y] (ab52629)

  • Datasheet
  • SDS
Reviews (4)Q&A (3)References (39)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
  • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
  • Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1556Y] to Scavenging Receptor SR-BI
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

こちらの製品もご検討ください

タンパク質
Product image
Recombinant human Scavenging Receptor SR-BI protein (Fc Chimera) (ab182680)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

製品の概要

  • 製品名

    Anti-Scavenging Receptor SR-BI antibody [EP1556Y]
    Scavenging Receptor SR-BI 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EP1556Y] to Scavenging Receptor SR-BI
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt or ICC
  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Sheep
  • 免疫原

    Synthetic peptide within Human Scavenging Receptor SR-BI aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: Q8WTV0

  • ポジティブ・コントロール

    • WB: Mouse liver tissue lysate. IHC-P: Human liver tissue.
  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EP1556Y
  • アイソタイプ

    IgG
  • 研究分野

    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Alternative Versions

    • Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)
    • HRP Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab206233)
    • Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - BSA and Azide free (ab271844)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Scavenging Receptor SR-BI protein (Fc Chimera) (ab182680)
  • Related Products

    • Sodium salicylate, anti-inflammatory agent (ab120746)

アプリケーション

Our Abpromise guarantee covers the use of ab52629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Detects a band of approximately 80 kDa.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt or ICC.
  • ターゲット情報

    • 機能

      Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester.
    • 組織特異性

      Widely expressed.
    • 配列類似性

      Belongs to the CD36 family.
    • 翻訳後修飾

      N-glycosylated.
    • 細胞内局在

      Cell membrane. Membrane > caveola. Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae.
    • Target information above from: UniProt accession Q8WTV0 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 949 Human
      • Entrez Gene: 20778 Mouse
      • Entrez Gene: 25073 Rat
      • Omim: 601040 Human
      • SwissProt: Q8WTV0 Human
      • SwissProt: Q61009 Mouse
      • SwissProt: P97943 Rat
      • Unigene: 520348 Human
      • Unigene: 282242 Mouse
      • Unigene: 88169 Rat
      see all
    • 別名

      • CD36 and LIMPII analogous 1 antibody
      • CD36 antibody
      • CD36 Antigen like 1 antibody
      • CD36 antigen-like 1 antibody
      • CD36L1 antibody
      • CLA 1 antibody
      • CLA-1 antibody
      • CLA1 antibody
      • Collagen type I receptor antibody
      • HDLQTL6 antibody
      • MGC138242 antibody
      • SCARB1 antibody
      • Scavebger Receptor Class B Member 1 antibody
      • Scavenger receptor class B member 1 antibody
      • Scavenger Receptor Class B Type 1 antibody
      • SCRB1_HUMAN antibody
      • SR BI antibody
      • SR-BI antibody
      • SRB1 antibody
      • SRBI antibody
      • Thrombospondin receptor like 1 antibody
      • thrombospondin receptor-like 1 antibody
      see all

    画像

    • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
      Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Scavenging Receptor SR-BI knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HepG2 whole cell lysate (20 µg)
      Lane 4: Human liver whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab52629 observed at 80 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab52629 was shown to specifically react with Scavenging Receptor SR-BI in wild-type HAP1 cells as signal was lost in Scavenging Receptor SR-BI knockout cells. Wild-type and Scavenging Receptor SR-BI knockout samples were subjected to SDS-PAGE. Ab52629 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
      Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
      All lanes : Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/1000 dilution (purified)

      Lane 1 : Mouse liver lysate
      Lane 2 : Rat liver lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Observed band size: 80 kDa
      why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
      Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
      All lanes : Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/1000 dilution (purified)

      Lane 1 : Human fetal liver lysate
      Lane 2 : HepG2 lysate
      Lane 3 : PC-3 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Observed band size: 80 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
      Immunohistochemical staining of paraffin embedded human liver with purified ab52629 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
      Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
      Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/2000 dilution (unpurified) + Mouse liver lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Observed band size: 80 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
      Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

      THP1 cells were incubated at 37ºC for 40h with vehicle control (0 µM) and different concentrations of sodium salicylate (ab120746). Decreased expression of scavenging receptor SR-BI in THP1 cells correlates with an increase in sodium salicylate concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with unpurified ab52629 at 1/2000 dilution and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

       

    • Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)
      Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (39)

    ab52629 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab52629 は 39 報の論文で使用されています。

    • Cruz W  et al. Lipoprotein-Like Nanoparticle Carrying Small Interfering RNA Against Spalt-Like Transcription Factor 4 Effectively Targets Hepatocellular Carcinoma Cells and Decreases Tumor Burden. Hepatol Commun 4:769-782 (2020). PubMed: 32363325
    • Tonini C  et al. Inhibition of Bromodomain and Extraterminal Domain (BET) Proteins by JQ1 Unravels a Novel Epigenetic Modulation to Control Lipid Homeostasis. Int J Mol Sci 21:N/A (2020). PubMed: 32075110
    • Singh AB  et al. FXR activation promotes intestinal cholesterol excretion and attenuates hyperlipidemia in SR-B1-deficient mice fed a high-fat and high-cholesterol diet. Physiol Rep 8:e14387 (2020). PubMed: 32170842
    • Hu PA  et al. Bromelain Confers Protection against the Non-Alcoholic Fatty Liver Disease in Male C57bl/6 Mice. Nutrients 12:N/A (2020). PubMed: 32443556
    • Subramani PA  et al. Plasmodium vivax liver stage assay platforms using Indian clinical isolates. Malar J 19:214 (2020). PubMed: 32571333
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-Scavenging Receptor SR-BI antibody [EP1556Y]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Goat Cell lysate - whole cell (mammary epithelial)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    80 µg
    Specification
    mammary epithelial
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More

    Qamar Taban

    Verified customer

    投稿 Aug 28 2020

    Western blot abreview for Anti-Scavenging Receptor SR-BI antibody [EP1556Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Spleen)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    40 µg
    Specification
    Spleen
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 21 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Scavenging Receptor SR-BI antibody [EP1556Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293T)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    HEK293T
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 14 2017

    Question


    Inquiry: loading 40ug cell lysat from BAEC, prepared 2 identical blots probing blot 2 (lower) with ab24603 as a replacement for ab3, dilution 1:1000 Result: antibody not specific blot 1 (upper blot) probing with ab52629 dilution 1:2000 - excellent antibody!

    Read More

    Abcam community

    Verified customer

    Asked on Dec 12 2012

    Answer

    EThank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody, particularly as the previous one you were using succesfully is no longer available.

    I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB and Cow, Mouse and Rat samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

    I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

    I would appreciate if you could also provide an image which would help us to assess the results.

    Thank you for your time and cooperation. We look forward to receiving the completed questionaire.


    Order Details
    Antibody code:

    Problem
    Choose: Non-specific band Multiple bands No signal or weak signal High background

    Lot number

    Purchase order number
    or preferably Abcam order number:



    General Information
    Antibody storage conditions (temperature/reconstitution etc)


    Description of the problem (high background, wrong band size, more bands, no band etc.)


    Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)


    Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)


    Amount of protein loaded


    Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)


    Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)


    Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


    Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


    Detection method (ECL, ECLPlus etc.)


    Positive and negative controls used (please specify)



    Optimization attempts (problem solving)
    How many times have you tried the Western?



    Have you run a "No Primary" control?
    Yes No

    Do you obtain the same results every time?
    Yes No
    e.g. are the background bands always in the same place?


    What steps have you altered?


    Additional Notes:


    Image:
    We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.

    Read More

    Abcam Scientific Support

    Answered on Dec 12 2012

    Question

    Phone call requesting information on the concentration of Anti-Scavenging Receptor SR-BI antibody (ab52629), lot number GR52658-2.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 19 2012

    Answer

    Thank you for contacting us this morning.

    I can now confirm that the concentration of Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629), lot number GR52658-2 is0.223 mg/mL.

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    Answered on Mar 19 2012

    Question

    Hi I would like to use the following antibody: srb1 (ab52629). Could you provide the blasting results of your sequence immunogen against Rattus Norvegicus? thank in advance best

    Read More

    Abcam community

    Verified customer

    Asked on Feb 17 2012

    Answer

    Thank you for contacting us. This product has an 85% homolgy between the immunogen and rat protein. The sequence is I have plaved a link to blastp for rat below: http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch&PROG_DEF=blastn&BLAST_PROG_DEF=megaBlast&SHOW_DEFAULTS=on&BLAST_SPEC=OGP__10116__10621 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 18 2012

    Flow Cytometry abreview for Anti-Scavenging Receptor SR-BI antibody [EP1556Y]

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    Application
    Flow Cytometry
    Sample
    Mouse Cell (macrophage cell line RAW267.4)
    Permeabilization
    No
    Gating Strategy
    macrophages
    Specification
    macrophage cell line RAW267.4
    Preparation
    Cell harvesting/tissue preparation method: by cell scraper
    Sample buffer: 1x PBS
    Fixation
    Formaldehyde
    Read More

    Mr. Pijus Barman

    Verified customer

    投稿 May 17 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
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