Anti-Scavenging Receptor SR-BI 抗体 [EP1556Y] (ab52629)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1556Y] to Scavenging Receptor SR-BI
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
製品の概要
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製品名
Anti-Scavenging Receptor SR-BI antibody [EP1556Y]
Scavenging Receptor SR-BI 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1556Y] to Scavenging Receptor SR-BI -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Sheep -
免疫原
Synthetic peptide within Human Scavenging Receptor SR-BI aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: Q8WTV0 -
ポジティブ・コントロール
- WB: Mouse liver tissue lysate. IHC-P: Human liver tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1556Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab52629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | 1/1000 - 1/2000. Detects a band of approximately 80 kDa. | |
IHC-P | 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the CD36 family. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Cell membrane. Membrane > caveola. Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae. - Information by UniProt
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参照データベース
- Entrez Gene: 949 Human
- Entrez Gene: 20778 Mouse
- Entrez Gene: 25073 Rat
- Omim: 601040 Human
- SwissProt: Q8WTV0 Human
- SwissProt: Q61009 Mouse
- SwissProt: P97943 Rat
- Unigene: 520348 Human
see all -
別名
- CD36 and LIMPII analogous 1 antibody
- CD36 antibody
- CD36 Antigen like 1 antibody
see all
画像
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Scavenging Receptor SR-BI knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: Human liver whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab52629 observed at 80 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab52629 was shown to specifically react with Scavenging Receptor SR-BI in wild-type HAP1 cells as signal was lost in Scavenging Receptor SR-BI knockout cells. Wild-type and Scavenging Receptor SR-BI knockout samples were subjected to SDS-PAGE. Ab52629 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/1000 dilution (purified)
Lane 1 : Mouse liver lysate
Lane 2 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Observed band size: 80 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
All lanes : Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/1000 dilution (purified)
Lane 1 : Human fetal liver lysate
Lane 2 : HepG2 lysate
Lane 3 : PC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Observed band size: 80 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)Immunohistochemical staining of paraffin embedded human liver with purified ab52629 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/2000 dilution (unpurified) + Mouse liver lysate at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Observed band size: 80 kDa why is the actual band size different from the predicted? -
THP1 cells were incubated at 37ºC for 40h with vehicle control (0 µM) and different concentrations of sodium salicylate (ab120746). Decreased expression of scavenging receptor SR-BI in THP1 cells correlates with an increase in sodium salicylate concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with unpurified ab52629 at 1/2000 dilution and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (39)
ab52629 は 39 報の論文で使用されています。
- Cruz W et al. Lipoprotein-Like Nanoparticle Carrying Small Interfering RNA Against Spalt-Like Transcription Factor 4 Effectively Targets Hepatocellular Carcinoma Cells and Decreases Tumor Burden. Hepatol Commun 4:769-782 (2020). PubMed: 32363325
- Tonini C et al. Inhibition of Bromodomain and Extraterminal Domain (BET) Proteins by JQ1 Unravels a Novel Epigenetic Modulation to Control Lipid Homeostasis. Int J Mol Sci 21:N/A (2020). PubMed: 32075110
- Singh AB et al. FXR activation promotes intestinal cholesterol excretion and attenuates hyperlipidemia in SR-B1-deficient mice fed a high-fat and high-cholesterol diet. Physiol Rep 8:e14387 (2020). PubMed: 32170842
- Hu PA et al. Bromelain Confers Protection against the Non-Alcoholic Fatty Liver Disease in Male C57bl/6 Mice. Nutrients 12:N/A (2020). PubMed: 32443556
- Subramani PA et al. Plasmodium vivax liver stage assay platforms using Indian clinical isolates. Malar J 19:214 (2020). PubMed: 32571333