Anti-SCAMP5 抗体 (ab3432)
Key features and details
- Rabbit polyclonal to SCAMP5
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-SCAMP5 antibody
SCAMP5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SCAMP5 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse, Human
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免疫原
Synthetic peptide corresponding to Rat SCAMP5 aa 217-233.
Sequence:NQPQTQYSATPNYTYSN
(Peptide available asab4971) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3432の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa.
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| 特記事項 |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa. |
ターゲット情報
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機能
Required for the calcium-dependent exocytosis of signal sequence-containing cytokines such as CCL5. Probably acts in cooperation with the SNARE machinery. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) in case of endoplasmic reticulum stress by inhibiting the endocytosis pathway. -
組織特異性
Expressed both by neuronal and nonneuronal tissues. Expressed in brain, stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and in the different parts of brain. In thyroid tissues, it is expressed by the follicular epithelial cells. In the adrenal gland tissues it is detected in the zona fasciculate of the cortex region (at protein level). -
配列類似性
Belongs to the SCAMP family. SCAMP5 subfamily. -
細胞内局在
Cell membrane. Golgi apparatus membrane. Golgi apparatus > trans-Golgi network membrane. Recycling endosome membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Mainly localizes in Golgi apparatus membrane. Upon calcium-triggered exocytosis, it translocates to the cell membrane. Highly enriched in synaptic vesicles. - Information by UniProt
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参照データベース
- Entrez Gene: 192683 Human
- Entrez Gene: 56807 Mouse
- Entrez Gene: 65171 Rat
- Omim: 613766 Human
- SwissProt: Q8TAC9 Human
- SwissProt: Q9JKD3 Mouse
- SwissProt: Q9JKE3 Rat
- Unigene: 374180 Human
see all -
別名
- AI426171 antibody
- AW558254 antibody
- DKFZp686L1799 antibody
see all
画像
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Western blot - Anti-SCAMP5 antibody (ab3432)Anti-SCAMP5 antibody (ab3432) + Rat brain extract
Observed band size: 25 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab3432 は 2 報の論文で使用されています。
- Yang Y et al. Secretory carrier membrane protein 5 is an autophagy inhibitor that promotes the secretion of a-synuclein via exosome. PLoS One 12:e0180892 (2017). WB ; Rat, Human . PubMed: 28700687
- Noh JY et al. SCAMP5 Links Endoplasmic Reticulum Stress to the Accumulation of Expanded Polyglutamine Protein Aggregates via Endocytosis Inhibition. J Biol Chem 284:11318-25 (2009). ICC/IF . PubMed: 19240033