Anti-SCAMP5 抗体 (ab3432)

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Western blot - Anti-SCAMP5 antibody (ab3432)

    Key features and details

    • Rabbit polyclonal to SCAMP5
    • Suitable for: WB
    • Reacts with: Rat, Cow
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-SCAMP5 antibody
      SCAMP5 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to SCAMP5

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Rat, Cow
      交差が予測される動物種: Mouse, Human

    • 免疫原

      Synthetic peptide corresponding to Rat SCAMP5 aa 217-233.
      Sequence:

      NQPQTQYSATPNYTYSN


      (Peptide available as ab4971)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab3432 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa.

    ターゲット情報

    • 機能

      Required for the calcium-dependent exocytosis of signal sequence-containing cytokines such as CCL5. Probably acts in cooperation with the SNARE machinery. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) in case of endoplasmic reticulum stress by inhibiting the endocytosis pathway.

    • 組織特異性

      Expressed both by neuronal and nonneuronal tissues. Expressed in brain, stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and in the different parts of brain. In thyroid tissues, it is expressed by the follicular epithelial cells. In the adrenal gland tissues it is detected in the zona fasciculate of the cortex region (at protein level).

    • 配列類似性

      Belongs to the SCAMP family. SCAMP5 subfamily.

    • 細胞内局在

      Cell membrane. Golgi apparatus membrane. Golgi apparatus > trans-Golgi network membrane. Recycling endosome membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Mainly localizes in Golgi apparatus membrane. Upon calcium-triggered exocytosis, it translocates to the cell membrane. Highly enriched in synaptic vesicles.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • AI426171 antibody
      • AW558254 antibody
      • DKFZp686L1799 antibody
      • hSCAMP5 antibody
      • MGC24969 antibody
      • Sc5 antibody
      • SCAM5_HUMAN antibody
      • Scamp5 antibody
      • Secretory carrier associated membrane protein 5 antibody
      • Secretory carrier membrane protein 5 antibody
      • Secretory carrier-associated membrane protein 5 antibody
      see all

    画像

    参考文献 (2)

    ab3432 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3432 は 2 報の論文で使用されています。

    • Yang Y  et al. Secretory carrier membrane protein 5 is an autophagy inhibitor that promotes the secretion of a-synuclein via exosome. PLoS One 12:e0180892 (2017). WB ; Rat, Human . PubMed: 28700687
    • Noh JY  et al. SCAMP5 Links Endoplasmic Reticulum Stress to the Accumulation of Expanded Polyglutamine Protein Aggregates via Endocytosis Inhibition. J Biol Chem 284:11318-25 (2009). ICC/IF . PubMed: 19240033

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-SCAMP5 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    15 µg
    Specification
    Brain
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 14 2018

    Western blot abreview for Anti-SCAMP5 antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Microglial Cell Line)
    Gel Running Conditions
    Reduced Denaturing (14%)
    Loading amount
    100 µg
    Specification
    Human Microglial Cell Line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Dr. David Brown

    Verified customer

    投稿 Jan 12 2018

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