Anti-SCAMP5 抗体 (ab3432)

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Key features and details

  • Rabbit polyclonal to SCAMP5
  • Suitable for: WB
  • Reacts with: Rat, Cow
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-SCAMP5 antibody
    SCAMP5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to SCAMP5

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Rat, Cow
    交差が予測される動物種: Mouse, Human

  • 免疫原

    Synthetic peptide corresponding to Rat SCAMP5 aa 217-233.
    Sequence:

    NQPQTQYSATPNYTYSN


    (Peptide available as ab4971)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa.

ターゲット情報

  • 機能

    Required for the calcium-dependent exocytosis of signal sequence-containing cytokines such as CCL5. Probably acts in cooperation with the SNARE machinery. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) in case of endoplasmic reticulum stress by inhibiting the endocytosis pathway.

  • 組織特異性

    Expressed both by neuronal and nonneuronal tissues. Expressed in brain, stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and in the different parts of brain. In thyroid tissues, it is expressed by the follicular epithelial cells. In the adrenal gland tissues it is detected in the zona fasciculate of the cortex region (at protein level).

  • 配列類似性

    Belongs to the SCAMP family. SCAMP5 subfamily.

  • 細胞内局在

    Cell membrane. Golgi apparatus membrane. Golgi apparatus > trans-Golgi network membrane. Recycling endosome membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Mainly localizes in Golgi apparatus membrane. Upon calcium-triggered exocytosis, it translocates to the cell membrane. Highly enriched in synaptic vesicles.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AI426171 antibody
    • AW558254 antibody
    • DKFZp686L1799 antibody
    • hSCAMP5 antibody
    • MGC24969 antibody
    • Sc5 antibody
    • SCAM5_HUMAN antibody
    • Scamp5 antibody
    • Secretory carrier associated membrane protein 5 antibody
    • Secretory carrier membrane protein 5 antibody
    • Secretory carrier-associated membrane protein 5 antibody
    see all

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参考文献 (2)

ab3432 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3432 は 2 報の論文で使用されています。

  • Yang Y  et al. Secretory carrier membrane protein 5 is an autophagy inhibitor that promotes the secretion of a-synuclein via exosome. PLoS One 12:e0180892 (2017). WB ; Rat, Human . PubMed: 28700687
  • Noh JY  et al. SCAMP5 Links Endoplasmic Reticulum Stress to the Accumulation of Expanded Polyglutamine Protein Aggregates via Endocytosis Inhibition. J Biol Chem 284:11318-25 (2009). ICC/IF . PubMed: 19240033

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Western blot abreview for Anti-SCAMP5 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
15 µg
Specification
Brain
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C
Read More

Abcam user community

Verified customer

投稿 Nov 14 2018

Western blot abreview for Anti-SCAMP5 antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Microglial Cell Line)
Gel Running Conditions
Reduced Denaturing (14%)
Loading amount
100 µg
Specification
Human Microglial Cell Line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Dr. David Brown

Verified customer

投稿 Jan 12 2018

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