Anti-SATB2 抗体 [SATBA4B10] - C-terminal (ab51502)

製品の概要

  • 製品名
    Anti-SATB2 antibody [SATBA4B10] - C-terminal
    SATB2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [SATBA4B10] to SATB2 - C-terminal
  • 由来種
    Mouse
  • アプリケーション
    適用あり: IP, ICC/IF, IHC-FoFr, WB, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Zebrafish
  • 免疫原

    Recombinant fragment corresponding to Human SATB2 (C terminal).

  • ポジティブ・コントロール
    • ICC/IF: HeLa cells. WB: NIH/3T3 whole cell lysate. IP: SATB2 IP in NIH/3T3 whole cell lysate.
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
  • バッファー
    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    Purified from TCS
  • ポリ/モノ
    モノクローナル
  • クローン名
    SATBA4B10
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab51502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP 1/100.
ICC/IF 1/5.
IHC-FoFr Use at an assay dependent concentration.
WB 1/100. Predicted molecular weight: 81 kDa.
IHC-Fr 1/50 - 1/400.
IHC-P 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.
  • 組織特異性
    High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.
  • 関連疾患
    Note=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11).
    Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis.
    Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22).
  • 配列類似性
    Belongs to the CUT homeobox family.
    Contains 2 CUT DNA-binding domains.
    Contains 1 homeobox DNA-binding domain.
  • 翻訳後修飾
    Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.
  • 細胞内局在
    Nucleus matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA binding protein SATB2 antibody
    • DNA-binding protein SATB2 antibody
    • FLJ21474 antibody
    • FLJ32076 antibody
    • GLSS antibody
    • KIAA1034 antibody
    • MGC119474 antibody
    • MGC119477 antibody
    • SATB family member 2 antibody
    • SATB homeobox 2 antibody
    • SATB2 antibody
    • SATB2_HUMAN antibody
    • Special AT rich sequence binding protein 2 antibody
    • Special AT-rich sequence-binding protein 2 antibody
    see all

画像

  • ab51502 staining SATB2 in human fetal cortex tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using 10 mM citrate buffer pH 6.0. Samples were then blocked with 10% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/100 dilution for 24 hours at 4°C. A Cy3®-conjugated horse anti-mouse polyclonal was used as secondary antibody at a 1/200 dilution. Counterstained with DAPI (blue).

    See Abreview

  • ab51502 staining SATB2 (red) in a mouse cortex e17.5 cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 3% donkey serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/10) for 90 minutes at 22°C. An Alexa Fluor®555-conjugated Donkey anti-mouse polyclonal (1/200) was used as the secondary antibody.
    Green: CTIP2 (ab18465) staining
    Blue: TBR1 (ab31940) staining

    See Abreview

  • ab51502 staining cultured rat cortical neuron cells by ICC/IF.

    Cells were fixed in PFA, permeabilized in 0.1% triton and blocked with 10% BSA for 1 hour at 22°C.  The sample was then incubated with ab51502, diluted 1/25 for 2 hours at 22°C prior to incubation with a Cy3® conjugated donkey anti-mouse antibody, diluted 1/400.

    Red: SATB2

    Green: F-actin

    See Abreview

  • ab51502 staining SATB2 in mouse brain tissue sections by IHC-Fr (Frozen sections).

    Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat polyclonal to mouse IgG (1/1000) was used as secondary antibody.

    See Abreview

  • ab51502 at a 1/200 dilution staining SATB2 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunocytochemistry.

  • Anti-SATB2 antibody [SATBA4B10] - C-terminal (ab51502) at 1/100 dilution + NIH/3T3 (Mouse embryo fibroblast cell line whole cell lysate at 25 µg

    Secondary
    anti Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 81 kDa
    Observed band size: 85 kDa (why is the actual band size different from the predicted?)



    Same results obtained using HT1080 (Human fibrosarcoma cell line) whole cell lysate at 25 µg.

  • Immunoprecipitation using ab51502 at 1:100 dilution.

    Sample: NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate 25 µg.

    Secondary used at a 1:2500 dilution.

参考文献

This product has been referenced in:
  • Bishop HI  et al. Kv2 Ion Channels Determine the Expression and Localization of the Associated AMIGO-1 Cell Adhesion Molecule in Adult Brain Neurons. Front Mol Neurosci 11:1 (2018). Read more (PubMed: 29403353) »
  • Boucherie C  et al. Neural progenitor fate decision defects, cortical hypoplasia and behavioral impairment in Celsr1-deficient mice. Mol Psychiatry 23:723-734 (2018). IHC (PFA fixed) ; Mouse . Read more (PubMed: 29257130) »

See all 87 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human cerebral organoid)
Permeabilization
Yes - 0.25% Triton X-100
Specification
Human cerebral organoid
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 07 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Ahmar Aziz

Verified customer

投稿 Jul 08 2016

Application
Immunohistochemistry (Frozen sections)
Blocking step
NGS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - Triton-X 0.5%
Fixative
Paraformaldehyde
Username

Marcos Romualdo Costa

Verified customer

投稿 Jul 18 2014

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step
None
Sample
Rat Tissue sections (E9.5 rat placenta)
Specification
E9.5 rat placenta
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain 50um section)
Specification
Brain 50um section
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
Yes - 0.3% Triton X-100 in blocking step
Blocking step
Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jul 31 2012

Of the two rabbit monoclonals, ab92446 (SATB2) and ab92307 (SATB1), ab92446 is more likely to be specific for its target, SATB2, than ab92307 is for SATB1.

Click here (or use the following: https://www.abcam.com/index.html?datasheet=92446). ...

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Thank you for sending the catalogue abID's.

The alignments of the immunogens with the other proteins do suggest that these antibodies may not have the specificity you hope for:

The ab92307 immunogen shares 73% identity (11/15) wit...

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Thank you for contacting us. The concentration of ab51502 is 0.1 mg/ml and should be used at a dilution of 1/100 for both IP and WB. Regarding ab92445, this is a tissue culture supernatant antibody so the concentration is not determined. Generally conc...

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Application
Immunohistochemistry (Frozen sections)
Sample
Zebra finch Tissue sections (embryonal spinal cord)
Specification
embryonal spinal cord
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1TX100 in PBS
Blocking step
Roti-Immunoblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Dr. Robert Storm

Verified customer

投稿 Feb 06 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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