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Recombinant fragment corresponding to Human SATB2 (C terminal).
This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Our Abpromise guarantee covers the use of ab51502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration.|
|WB||1/100. Predicted molecular weight: 81 kDa.|
|IHC-Fr||1/50 - 1/400.|
|IHC-P||1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab51502 staining SATB2 in human fetal cortex tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using 10 mM citrate buffer pH 6.0. Samples were then blocked with 10% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/100 dilution for 24 hours at 4°C. A Cy3®-conjugated horse anti-mouse polyclonal was used as secondary antibody at a 1/200 dilution. Counterstained with DAPI (blue).
ab51502 staining SATB2 (red) in a mouse cortex e17.5 cells by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 3% donkey serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/10) for 90 minutes at 22°C. An Alexa Fluor®555-conjugated Donkey anti-mouse polyclonal (1/200) was used as the secondary antibody.
Green: CTIP2 (ab18465) staining
Blue: TBR1 (ab31940) staining
ab51502 staining cultured rat cortical neuron cells by ICC/IF.
Cells were fixed in PFA, permeabilized in 0.1% triton and blocked with 10% BSA for 1 hour at 22°C. The sample was then incubated with ab51502, diluted 1/25 for 2 hours at 22°C prior to incubation with a Cy3® conjugated donkey anti-mouse antibody, diluted 1/400.
ab51502 staining SATB2 in mouse brain tissue sections by IHC-Fr (Frozen sections).
Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat polyclonal to mouse IgG (1/1000) was used as secondary antibody.
ab51502 at a 1/200 dilution staining SATB2 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunocytochemistry.
Same results obtained using HT1080 (Human fibrosarcoma cell line) whole cell lysate at 25 µg.
Immunoprecipitation using ab51502 at 1:100 dilution.
Sample: NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate 25 µg.
Secondary used at a 1:2500 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"