Anti-SATB2 抗体 [EPNCIR130A] (ab92446)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR130A] to SATB2
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SATB2 antibody [EPNCIR130A]
SATB2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPNCIR130A] to SATB2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
適用なし: IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human SATB2. The exact sequence is proprietary.
Database link: Q9UPW6 -
ポジティブ・コントロール
- WB: HT-1080, SW1353, MCF7 and Saos-2 cell lysates; Rat and mouse brain and human fetal brain tissue lysates; Wild-type HAP1 whole cell lysate. IHC-P: Human cerebral cortex tissue; Mouse brain tissue. ICC/IF: Saos-2 cells Flow Cyt (intra): SH-SY5Y cells. IHC-Fr: Mouse brain tissue.
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特記事項
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of John Niederhuber. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPNCIR130A -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 488 Anti-SATB2 antibody [EPNCIR130A] (ab196316)
- Alexa Fluor® 647 Anti-SATB2 antibody [EPNCIR130A] (ab196536)
- Alexa Fluor® 594 Anti-SATB2 antibody [EPNCIR130A] (ab207040)
- Anti-SATB2 antibody [EPNCIR130A] - BSA and Azide free (ab212177)
- APC Anti-SATB2 antibody [EPNCIR130A] (ab225051)
- PE Anti-SATB2 antibody [EPNCIR130A] (ab225052)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
- MCF7 nuclear extract lysate (ab14860)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
- SW 1353 whole cell lysate (ab7915)
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92446の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 81 kDa.
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IHC-P |
1/150 - 1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/100.
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Flow Cyt (Intra) |
1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 81 kDa. |
IHC-P
1/150 - 1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. -
組織特異性
High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus. -
関連疾患
Note=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11).
Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis.
Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22). -
配列類似性
Belongs to the CUT homeobox family.
Contains 2 CUT DNA-binding domains.
Contains 1 homeobox DNA-binding domain. -
翻訳後修飾
Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity. -
細胞内局在
Nucleus matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 23314 Human
- Entrez Gene: 212712 Mouse
- Entrez Gene: 501145 Rat
- Omim: 608148 Human
- SwissProt: Q9UPW6 Human
- SwissProt: Q8VI24 Mouse
- Unigene: 145599 Mouse
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別名
- DNA binding protein SATB2 antibody
- DNA-binding protein SATB2 antibody
- FLJ21474 antibody
see all
画像
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All lanes : Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate at 20 µg
Lane 2 : SATB2 knockout HAP1 cell lysate at 20 µg
Lane 3 : Mouse E18 Embyonic brain cell lysate at 20 µg
Lane 4 : NIH/3T3 cell lysate at 20 µg
Lane 5 : HT1080 cell lysate at 20 µg
Lane 6 : Saos-2 cell lysate at 20 µg
Lanes 7 & 9 : Empty
Lane 8 : SATB1 Recombinant Protein cell lysate at 0.1 µg
Lane 10 : SATB2 Recombinant Protein (ab132405) cell lysate at 0.1 µg
Secondary
All lanes : Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-SATB2 antibody [EPNCIR130A] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92446 was shown to bind specifically to SATB2. A band was observed at 100 kDa in wild-type HAP1 cell lysates with no signal observed at this size in SATB2 knockout cell line.
To generate this image, wild-type and SATB2 knockout HAP1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Immunocytochemistry/Immunofluorescence analysis of Saos-2 cells (human osteosarcoma cell line) labelling SATB2 (red) with purified ab92446 at 1/100 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150083, an Alexa Fluor® 647-conjugated goat anti-rabbit IgG H&L (1/1000), was used as the secondary antibody. Tubulin (green) was stained with ab7291, an anti-alpha tubulin antibody (1/1000). DAPI (blue) was used as the nuclear counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling SATB2 with purified ab92446 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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All lanes : Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : SATB2 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 81 kDaLanes 1 - 2: Merged signal (red and green). Green - ab92446 observed at 83 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab92446 was shown to specifically react with SATB2 in wild-type HAP1 cells as signal was lost in SATB2 knockout cells. Wild-type and SATB2 knockout samples were subjected to SDS-PAGE. Ab92446 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Intracellular Flow Cytometry analysis of SH-SY5Y cells (human neuroblastoma cell line from bone marrow) labeling SATB2 with purified ab92446 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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All lanes : Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/10000 dilution
Lane 1 : Saos-2 (Human osteosarcoma epithelial) whole cell fresh lysate
Lane 2 : Saos-2 (Human osteosarcoma epithelial) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
Freshly made lysate is highly recommended to minimize protein degradation.
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Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution (purified) + SW1353 (human chondrosarcoma cell line) cell lysate at 20 µg
Secondary
Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 81 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution (purified)
Lane 1 : Saos-2 (human osteosarcoma cell line) cell lysate
Lane 2 : Human fetal brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 81 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 81 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution (unpurified)
Lane 1 : HT1080 (human fibrosarcoma cell line) cell lysate
Lane 2 : SW1353 (human chondrosarcoma cell line) cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 81 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (55)
ab92446 は 55 報の論文で使用されています。
- Liau ES et al. Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons. Nat Commun 14:46 (2023). PubMed: 36596814
- Sui X et al. Hsa_circ_0006677 regulates special AT-rich binding protein-2-mediated tumor-suppressive effect via functioning as a miR-1245a sponge in non-small cell lung cancer. Bioengineered 13:3760-3774 (2022). PubMed: 35081869
- Gu W et al. Rapid establishment of human colonic organoid knockout lines. STAR Protoc 3:101308 (2022). PubMed: 35463469
- Krzyspiak J et al. Potential Variables for Improved Reproducibility of Neuronal Cell Grafts at Stroke Sites. Cells 11:N/A (2022). PubMed: 35626693
- Chen G et al. GIGYF1 disruption associates with autism and impaired IGF-1R signaling. J Clin Invest 132:N/A (2022). PubMed: 35917186