Anti-SARS-CoV-2 Spike Glycoprotein S1 抗体 [HL6] - BSA and Azide free (ab281311)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HL6] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, Indirect ELISA, Sandwich ELISA
製品の概要
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製品名
Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free
SARS-CoV-2 Spike Glycoprotein S1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [HL6] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-P, Flow Cyt, Indirect ELISA, Sandwich ELISAmore details -
種交差性
交差種: SARS-CoV-2 -
免疫原
Recombinant fragment within Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. SARS-CoV-2 (strain Wuhan-Hu-1)
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ポジティブ・コントロール
- IHC-P: SARS-CoV-2 (COVID-19) infected human placenta an human lung tissue. Flow cyt: SARS-CoV-2 (2019-nCoV) Spike 293T cells. WB: SARS-CoV-2 Spike transfected 293T cells. ICC/IF: SARS-CoV-2 Spike transfected 293T cells. ELISA: Recombinant full-length SARS-CoV-2 spike protein, SARS-CoV-2 spike (full length) transfected 293T whole cell extracts.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
HL6 -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab281311の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100 - 1/1000.
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WB |
1/1000 - 1/10000.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cyt |
1/50.
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Indirect ELISA |
Use a concentration of 1 µg/ml.
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Sandwich ELISA |
Use a concentration of 1 µg/ml.
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特記事項 |
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ICC/IF
1/100 - 1/1000. |
WB
1/1000 - 1/10000. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt
1/50. |
Indirect ELISA
Use a concentration of 1 µg/ml. |
Sandwich ELISA
Use a concentration of 1 µg/ml. |
画像
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All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311) at 1/5000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : SARS-CoV Spike transfected 293T whole cell extrac
Lane 3 : SARS-CoV-2 (COVID-19) Spike transfected 293T whole cell extract
Lane 4 : MERS Spike transfected 293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody5% SDS-PAGE
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Paraffin embedded SARS-CoV-2 (COVID-19) infected human placenta (left) and normal human placenta (right) tissue stained for SARS-CoV-2 (COVID-19) nucleocapsid protein using ab281311 in immunohistochemical analysis.
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Paraffin embedded SARS-CoV-2 (COVID-19) infected human lung tissue stained for SARS-CoV-2 (COVID-19) nucleocapsid protein using ab281311 in immunohistochemical analysis.
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Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with ab281311 (2.4*10⁻⁶ ~ 20 nM). HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.
EC50: 0.251 nM -
Sandwich ELISA detection of non-transfected and SARS-CoV-2 spike (full length) transfected 293T whole cell extracts using 5 µg/mL of capture antibody and ab281311 as detection antibody at 1 µg/mL. HRP-conjugated Rabbit IgG antibody at 1/10000 dilution was used to detect the primary antibody.
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Mock and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) Spike S1 stained by ab281311 at 1/1000 dilution in immunohistochemical analysis. Fluoroshield with DAPI (blue).
Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 15 minutes.
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Mock and SARS-CoV-2 Spike-transfected 293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) spike stained (green) by ab281311 at 1/1000 dilution in Immunocytochemical analysis. Fluoroshield with DAPI (blue).
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Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein or recombinant SARS-CoV spike S1 subunit protein (62.5-4000 ng/mL). Coated protein probed with ab281311 at 1 µg/mL. HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.
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ab281311 detects SARS-CoV-2 (COVID-19) spike protein by flow cytometry analysis. SARS-CoV-2 (2019-nCoV) Spike 293T cells. Unlabelled sample was used as a control (blue) and ab281311 at 1/50 dilution (red). Acquisition of 20,000 events were collected for flow cytometry analysis.
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All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311) at 1/5000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : SARS-CoV-2 (COVID-19) Spike transfected 293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody5% SDS-PAGE
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab281311 は 1 報の論文で使用されています。
- Kamel M et al. Non-Invasive Detection of SARS-CoV-2 Antigen in Saliva versus Nasopharyngeal Swabs Using Nanobodies Conjugated Gold Nanoparticles. Trop Med Infect Dis 7:N/A (2022). PubMed: 35736981