Anti-SARS-CoV-2 envelope protein 抗体 - Coronavirus (ab272503)
Key features and details
- Rabbit polyclonal to SARS-CoV-2 envelope protein - Coronavirus
- Suitable for: ELISA, IHC-P
- Isotype: IgG
製品の概要
-
製品名
Anti-SARS-CoV-2 envelope protein antibody - Coronavirus
SARS-CoV-2 envelope protein 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SARS-CoV-2 envelope protein - Coronavirus -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, IHC-Pmore details -
種交差性
交差種: SARS-CoV-2 -
免疫原
Synthetic peptide within Human coronavirus SARS-CoV-2 envelope protein aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. 10 amino acid peptide
Database link: QHD43418.1
(Peptide available asab273064) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272503の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
Use a concentration of 1 µg/ml.
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
特記事項 |
---|
ELISA
Use a concentration of 1 µg/ml. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of COVID-19 patient lung tissue labeling SARS-CoV-2 envelope protein with ab272503 at 1 µg/mL. Tissue was blocked with 10% serum for 1 hour at room temperature. Heat mediated antigen retrieval was performed using citrate buffer pH 6. Samples were incubated with the primary antibody overnight at 4°C. An HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/250). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Strong positive signal observed in macrophage cells of COVID-19 patient lung but not in non-COVID-19 patient lung.
-
A direct ELISA was performed using antigen or control peptide as coating antigen and ab272503 as the capture antibody at 1 μg/ml, followed by the secondary antibody goat anti-rabbit IgG HRP conjugate at 1/20000 dilution. Detection range was from 32ng/ml to 2000 ng/ml.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab272503 は論文での使用が確認できていません。