Anti-Sarcomeric Alpha Actinin 抗体 [EA-53] (ab9465)
![Western blot - Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)](https://www.abcam.co.jp/ps/products/9/ab9465/Images/ab9465-394444-anti-sarcomeric-alpha-actinin-antibody-ea-53-western-blot-skeletal-muscle-rat.jpg "Western blot - Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)")
Key features and details
- Mouse monoclonal [EA-53] to Sarcomeric Alpha Actinin
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Sarcomeric Alpha Actinin antibody [EA-53]
Sarcomeric Alpha Actinin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [EA-53] to Sarcomeric Alpha Actinin -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Rabbit
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免疫原
Full length native protein (purified) corresponding to Rabbit Sarcomeric Alpha Actinin. Purified rabbit skeletal α-actinin.
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ポジティブ･コントロール
- WB: Mouse and rat skeletal muscle and rat heart tissue lysates. IHC-P: Human skeletal muscle tissue.
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特記事項
This product was changed from ascites to tissue culture supernatant on 9th September 2019. Lot numbers higher than GR3258034 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section. -
バッファー
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Proprietary Purification -
特記事項（精製）
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
EA-53 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9465の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 10 - 20 µg/ml.
ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user. |
|
| WB | (2) |
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 10 - 20 µg/ml. ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user. |
|
WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa). |
ターゲット情報
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機能
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. -
組織特異性
Expressed in both skeletal and cardiac muscle. -
関連疾患
Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
配列類似性
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
細胞内局在
Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. - Information by UniProt
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参照データベース
- Entrez Gene: 88 Human
- Entrez Gene: 291245 Rat
- Omim: 102573 Human
- SwissProt: P35609 Human
- Unigene: 498178 Human
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別名
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
see all
画像
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Western blot - Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)Lane 1 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/500 dilution
Lane 2 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/1000 dilution
Lane 3 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/2000 dilution
Lane 4 : No antibody control
All lanes : Rat skeletal muscle extract
Secondary
All lanes : Goat Anti-Mouse IgG (Fab) (HRP) at 1/4000 dilution
Predicted band size: 103 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)](https://www.abcam.co.jp/ps/products/9/ab9465/Images/ab9465-355404-anti-sarcomeric-alpha-actinin-antibody-ea-53-immunohistochemistry-human-tongue.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)Immunohistochemistry formalin-fixed, paraffin-embedded human tongue labeling Alpha Actinin with ab9465 at 12 μg/mL.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (193)
ab9465 は 193 報の論文で使用されています。
- Hofemeier AD et al. Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis. Elife 10:N/A (2021). PubMed: 33459593
- Li H et al. Endothelial Klf2-Foxp1-TGFß signal mediates the inhibitory effects of simvastatin on maladaptive cardiac remodeling. Theranostics 11:1609-1625 (2021). PubMed: 33408770
- Podgurskaya AD et al. Cyclophosphamide arrhythmogenicitytesting using human-induced pluripotent stem cell-derived cardiomyocytes. Sci Rep 11:2336 (2021). PubMed: 33504826
- Xu Y et al. MBNL1 regulates isoproterenol-induced myocardial remodelling in vitro and in vivo. J Cell Mol Med 25:1100-1115 (2021). PubMed: 33295096
- Pettinato AM et al. Sarcomere function activates a p53-dependent DNA damage response that promotes polyploidization and limits in vivo cell engraftment. Cell Rep 35:109088 (2021). PubMed: 33951429