アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    sarcomeric-alpha-actinin-antibody-ea-53-ab9465.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins
Share by email

Anti-Sarcomeric Alpha Actinin 抗体 [EA-53] (ab9465)

  • Datasheet
  • SDS
Reviews (12)Q&A (12)References (193)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)

Key features and details

  • Mouse monoclonal [EA-53] to Sarcomeric Alpha Actinin
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human
  • Isotype: IgG1

こちらの製品もご検討ください

アッセイ
Product image
Resazurin Assay Kit - Cell Cytotoxicity (Fluorometric)  (ab112119)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
一次抗体
Product image
Anti-Cardiac Troponin T antibody [1C11] (ab8295)

関連製品

製品の概要

  • 製品名

    Anti-Sarcomeric Alpha Actinin antibody [EA-53]
    Sarcomeric Alpha Actinin 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [EA-53] to Sarcomeric Alpha Actinin
  • 由来種

    Mouse
  • アプリケーション

    適用あり: IHC-P, WBmore details
  • 種交差性

    交差種: Rat, Human
    交差が予測される動物種: Rabbit
  • 免疫原

    Full length native protein (purified) corresponding to Rabbit Sarcomeric Alpha Actinin. Purified rabbit skeletal α-actinin.

  • ポジティブ・コントロール

    • WB: Mouse and rat skeletal muscle and rat heart tissue lysates. IHC-P: Human skeletal muscle tissue.
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 9th September 2019. Lot numbers higher than GR3258034 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section.
  • バッファー

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Proprietary Purification
  • 特記事項(精製)

    Purified from tissue culture supernatant.
  • ポリ/モノ

    モノクローナル
  • クローン名

    EA-53
  • ミエローマ

    unknown
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    unknown
  • 研究分野

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9465の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 10 - 20 µg/ml.

ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.

WB (2)
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
特記事項
IHC-P
Use a concentration of 10 - 20 µg/ml.

ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.

WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).

ターゲット情報

  • 機能

    F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
  • 組織特異性

    Expressed in both skeletal and cardiac muscle.
  • 関連疾患

    Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • 配列類似性

    Belongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • 細胞内局在

    Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
  • Target information above from: UniProt accession P35609 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 88 Human
    • Entrez Gene: 291245 Rat
    • Omim: 102573 Human
    • SwissProt: P35609 Human
    • Unigene: 498178 Human
    • 別名

      • Actin binding protein antibody
      • Actinin alpha 2 antibody
      • ACTN 2 antibody
      • ACTN2 antibody
      • ACTN2_HUMAN antibody
      • Alpha actinin 2 antibody
      • Alpha actinin skeletal muscle antibody
      • Alpha actinin skeletal muscle isoform 2 antibody
      • Alpha-actinin skeletal muscle isoform 2 antibody
      • Alpha-actinin-2 antibody
      • CMD1AA antibody
      • F actin cross linking protein antibody
      • F-actin cross-linking protein antibody
      see all

    画像

    • Western blot - Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)
      Western blot - Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)
      Lane 1 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/500 dilution
      Lane 2 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/1000 dilution
      Lane 3 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/2000 dilution
      Lane 4 : No antibody control

      All lanes : Rat skeletal muscle extract

      Secondary
      All lanes : Goat Anti-Mouse IgG (Fab) (HRP) at 1/4000 dilution

      Predicted band size: 103 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)
      Immunohistochemistry formalin-fixed, paraffin-embedded human tongue labeling Alpha Actinin with ab9465 at 12 μg/mL.
       

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (193)

    ab9465 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9465 は 193 報の論文で使用されています。

    • Hofemeier AD  et al. Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis. Elife 10:N/A (2021). PubMed: 33459593
    • Li H  et al. Endothelial Klf2-Foxp1-TGFß signal mediates the inhibitory effects of simvastatin on maladaptive cardiac remodeling. Theranostics 11:1609-1625 (2021). PubMed: 33408770
    • Podgurskaya AD  et al. Cyclophosphamide arrhythmogenicitytesting using human-induced pluripotent stem cell-derived cardiomyocytes. Sci Rep 11:2336 (2021). PubMed: 33504826
    • Xu Y  et al. MBNL1 regulates isoproterenol-induced myocardial remodelling in vitro and in vivo. J Cell Mol Med 25:1100-1115 (2021). PubMed: 33295096
    • Pettinato AM  et al. Sarcomere function activates a p53-dependent DNA damage response that promotes polyploidization and limits in vivo cell engraftment. Cell Rep 35:109088 (2021). PubMed: 33951429
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 24 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Sarcomeric Alpha Actinin antibody [EA-53]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Neonatal cardiomyocyte)
    Permeabilization
    No
    Specification
    Neonatal cardiomyocyte
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 28 2020

    IHC - Wholemount abreview for Anti-Sarcomeric Alpha Actinin antibody [EA-53]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Rat Tissue (skeletal muscle)
    Specification
    skeletal muscle
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 29 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-Sarcomeric Alpha Actinin antibody [EA-53]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Sheep Tissue sections (Muscle)
    Permeabilization
    Yes - 0,2 % Triton-X in PBS
    Specification
    Muscle
    Fixative
    Methanol:Acetone 1:1
    Read More

    Frau. Kassandra Walluks

    Verified customer

    投稿 Apr 24 2018

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Sarcomeric Alpha Actinin antibody [EA-53]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 26 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Sarcomeric Alpha Actinin antibody [EA-53]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Cardiac cells)
    Specification
    Cardiac cells
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 16 2016

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Sarcomeric Alpha Actinin antibody [EA-53]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Heart)
    Antigen retrieval step
    None
    Permeabilization
    Yes - Triton x-100, 0.01%
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 05 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-Sarcomeric Alpha Actinin antibody [EA-53]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (heart)
    Permeabilization
    Yes - Triton x-100, 0.01%
    Specification
    heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 17 2015

    Western blot abreview for Anti-Sarcomeric Alpha Actinin antibody [EA-53]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Rat Tissue lysate - whole (heart)
    Specification
    heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    DR. Shyam Sundar Nandi

    Verified customer

    投稿 Nov 25 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Sarcomeric Alpha Actinin antibody [EA-53]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
    Sample
    Rat Cell (Heart)
    Specification
    Heart
    Permeabilization
    Yes - Triton x-100, 0.01%
    Fixative
    Paraformaldehyde
    Read More

    DR. Shyam Sundar Nandi

    Verified customer

    投稿 Nov 20 2014

    Question

    I refer to your Anti-Sarcomeric Alpha Actinin antibody [EA-53] antibody.

    Currently I am using paraffin mouse muscle sections and would like to label Z
    bands
    together with Myosin (MF20 a general muscle marker monoclonal mouse).
    However, your
    antibody was produced in the mouse as well.

    Therefore I would like to ask if it's possible for you can to send me sample
    of the antibody
    for 1-2 sections because I would try IgG1 and IgG2 specific secondary
    antibodies for a
    double-labeling? If it works I would definitely buy the antibody along with
    Stretavidin-HRP.

    Thank you very much. I'm looking forward to hearing from you soon.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 04 2012

    Answer

    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    In addition, we have a Polymer IHC Kit which tackles exactly the problem of a high background which arises from this kind of staining (mouse-on-mouse staining):

    https://www.abcam.com/Mouse-on-Mouse-Polymer-IHC-Kit-ab127055.html (or use the following: https://www.abcam.com/Mouse-on-Mouse-Polymer-IHC-Kit-ab127055.html).

    ab127055 allows the use of mouse antibodies on mouse tissue to obtain excellent signal intensities and clean background.

    Another alternative i could recommend would be using three antibodies in total: Use a Fab-fragment anti-mouse antibody against the H+L chain to block the endogenous mouse IgGs, than incubate with your primaries and (as you suggested) use an isotype specific secondary antibody.

    A Fab fragment antibody has the advantage that it can "cover" the endogenous mouse IgG tighter than a whole IgG antibody.

    https://www.abcam.com/Goat-Fab-Mouse-IgG-HL-ab6668.html (or use the following: https://www.abcam.com/Goat-Fab-Mouse-IgG-HL-ab6668.html).



    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Jul 04 2012

    1-10 of 24 Abreviews or Q&A

    •  戻る
    • 1
    • 2
    • 3
    • 次へ 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    • ターゲット別に見る
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2022 Abcam plc. All rights reserved.