Name: Anti-Sarcomeric Alpha Actinin antibody [EA-53]
SKU: ab9465
Rating: 4 (11 reviews)

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Key features and details

Mouse monoclonal [EA-53] to Sarcomeric Alpha Actinin
Suitable for: IHC-P, WB
Reacts with: Rat, Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Cat, Dog, Human, Pig, Xenopus laevis, Fish, Lizard, Snake
Isotype: IgG1

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二次抗体

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一次抗体 - Mouse Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)
IHC-P, WB

二次抗体 - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
WB, IP, ELISA, IHC-P

関連製品

製品名

Anti-Sarcomeric Alpha Actinin antibody [EA-53]
Sarcomeric Alpha Actinin 一次抗体製品一覧
製品の詳細

Mouse monoclonal [EA-53] to Sarcomeric Alpha Actinin
由来種

Mouse
アプリケーション

適用あり: IHC-P, WBmore details
種交差性

交差種: Rat, Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Cat, Dog, Human, Pig, Xenopus laevis, Fish, Lizard, Snake
免疫原

Full length native protein (purified) corresponding to Rabbit Sarcomeric Alpha Actinin. Purified rabbit skeletal α-actinin.
ポジティブ･コントロール
- WB: Mouse and rat skeletal muscle and rat heart tissue lysates. IHC-P: Human skeletal muscle tissue.
特記事項

This product was changed from ascites to tissue culture supernatant on 9^th September 2019. Lot numbers higher than GR3258034 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section.
バッファー

pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS
Concentration information loading...
精製度

Proprietary Purification
特記事項（精製）

Purified from tissue culture supernatant.
ポリ/モノ

モノクローナル
クローン名

EA-53
ミエローマ

unknown
アイソタイプ

IgG1
軽鎖の種類

unknown
研究分野

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
Related Products
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Our Abpromise guarantee covers the use of ab9465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション	Abreviews	特記事項
IHC-P		Use a concentration of 10 - 20 µg/ml. ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.
WB		Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).

機能

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
組織特異性

Expressed in both skeletal and cardiac muscle.
関連疾患

Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
配列類似性

Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats.
細胞内局在

Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
Target information above from: UniProt accession P35609 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 396263 Chicken
- Entrez Gene: 536607 Cow
- Entrez Gene: 88 Human
- Entrez Gene: 291245 Rat
- Entrez Gene: 432169 Xenopus laevis
- Omim: 102573 Human
- SwissProt: Q3ZC55 Cow
- SwissProt: P35609 Human
- Unigene: 498178 Human
see all
別名
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
- ACTN2 antibody
- ACTN2_HUMAN antibody
- Alpha actinin 2 antibody
- Alpha actinin skeletal muscle antibody
- Alpha actinin skeletal muscle isoform 2 antibody
- Alpha-actinin skeletal muscle isoform 2 antibody
- Alpha-actinin-2 antibody
- CMD1AA antibody
- F actin cross linking protein antibody
- F-actin cross-linking protein antibody
see all

Western blot - Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)

Lane 1 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/500 dilution
Lane 2 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/1000 dilution
Lane 3 : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/2000 dilution
Lane 4 : No antibody control

All lanes : Rat skeletal muscle extract

Secondary
All lanes : Goat Anti-Mouse IgG (Fab) (HRP) at 1/4000 dilution

Predicted band size: 103 kDa
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)

All lanes : Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) at 1/250 dilution

Lane 1 : Mouse skeletal muscle tissue lysate
Lane 2 : Rat skeletal muscle tissue lysate
Lane 3 : Rat heart tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 103 kDa
Observed band size: 103 kDa
Additional bands at: 25 kDa, 57 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 90 seconds

This image was generated using the ascites version of the product.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465)

Immunohistochemistry formalin-fixed, paraffin-embedded human tongue labeling Alpha Actinin with ab9465 at 12 μg/mL.

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

ab9465 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9465 は 123 報の論文で使用されています。

Chen X et al. The long noncoding RNA NR_045363 involves cardiomyocyte apoptosis and cardiac repair via p53 signal pathway. Cell Biol Int N/A:N/A (2020). PubMed: 32374467
Williams JL et al. Mylk3 null C57BL/6N mice develop cardiomyopathy, whereas Nnt null C57BL/6J mice do not. Life Sci Alliance 3:N/A (2020). PubMed: 32213617
Su T et al. Platelet-Inspired Nanocells for Targeted Heart Repair After Ischemia/Reperfusion Injury. Adv Funct Mater 29:N/A (2019). PubMed: 32256277
Skylar-Scott MA et al. Biomanufacturing of organ-specific tissues with high cellular density and embedded vascular channels. Sci Adv 5:eaaw2459 (2019). PubMed: 31523707
Salvarani N et al. The K219T-Lamin mutation induces conduction defects through epigenetic inhibition of SCN5A in human cardiac laminopathy. Nat Commun 10:2267 (2019). PubMed: 31118417

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-10 of 23 Abreviews or Q&A

Application

IHC - Wholemount

Sample

Rat Tissue (skeletal muscle)

Specification

skeletal muscle

Abcam user community

Verified customer

投稿 Mar 29 2019

Application

Immunohistochemistry (Frozen sections)

Sample

Sheep Tissue sections (Muscle)

Permeabilization

Yes - 0,2 % Triton-X in PBS

Specification

Muscle

Fixative

Methanol:Acetone 1:1

Frau. Kassandra Walluks

Verified customer

投稿 Apr 24 2018

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Mouse Tissue sections (Heart)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate Buffer

Permeabilization

Yes - 0.2% Triton X-100

Specification

Heart

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 Oct 26 2016

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Cardiac cells)

Specification

Cardiac cells

Blocking step

Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 Sep 16 2016

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Mouse Tissue sections (Heart)

Antigen retrieval step

None

Permeabilization

Yes - Triton x-100, 0.01%

Specification

Heart

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 Oct 05 2015

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (heart)

Permeabilization

Yes - Triton x-100, 0.01%

Specification

heart

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 Jun 17 2015

Application

Western blot

Loading amount

25 µg

Gel Running Conditions

Reduced Denaturing

Sample

Rat Tissue lysate - whole (heart)

Specification

heart

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Shyam Sundar Nandi

Verified customer

投稿 Nov 25 2014

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%

Sample

Rat Cell (Heart)

Specification

Heart

Permeabilization

Yes - Triton x-100, 0.01%

Fixative

Paraformaldehyde

Dr. Shyam Sundar Nandi

Verified customer

投稿 Nov 20 2014

Question

I refer to your Anti-Sarcomeric Alpha Actinin antibody [EA-53] antibody.

Currently I am using paraffin mouse muscle sections and would like to label Z
bands
together with Myosin (MF20 a general muscle marker monoclonal mouse).
However, your
antibody was produced in the mouse as well.

Therefore I would like to ask if it's possible for you can to send me sample
of the antibody
for 1-2 sections because I would try IgG1 and IgG2 specific secondary
antibodies for a
double-labeling? If it works I would definitely buy the antibody along with
Stretavidin-HRP.

Thank you very much. I'm looking forward to hearing from you soon.

Abcam community

Verified customer

Asked on Jul 04 2012

Answer

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

In addition, we have a Polymer IHC Kit which tackles exactly the problem of a high background which arises from this kind of staining (mouse-on-mouse staining):

https://www.abcam.com/index.html?datasheet=127055 (or use the following: https://www.abcam.com/index.html?datasheet=127055).

ab127055 allows the use of mouse antibodies on mouse tissue to obtain excellent signal intensities and clean background.

Another alternative i could recommend would be using three antibodies in total: Use a Fab-fragment anti-mouse antibody against the H+L chain to block the endogenous mouse IgGs, than incubate with your primaries and (as you suggested) use an isotype specific secondary antibody.

A Fab fragment antibody has the advantage that it can "cover" the endogenous mouse IgG tighter than a whole IgG antibody.

https://www.abcam.com/index.html?datasheet=6668 (or use the following: https://www.abcam.com/index.html?datasheet=6668).

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Abcam Scientific Support

Answered on Jul 04 2012

Question

What percentage of sodium azide is in the buffer of this antibody?

Abcam community

Verified customer

Asked on May 18 2012

Answer

Thank you for your call yesterday and for your patience while I've looked into your enquiry.

I've heard back from the lab, and there is 0.05% sodium azide in this antibody. I will update the datasheet and have an MSDS madepromptly. Please let me know if you have any further questions or if there is anything else that we can do for you, and I'll be happy to help.

Abcam Scientific Support

Answered on May 18 2012

1-10 of 23 Abreviews or Q&A

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Key features and details

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

特記事項（精製）

ポリ/モノ

クローン名

ミエローマ

アイソタイプ

軽鎖の種類

研究分野

関連製品

Compatible Secondaries

Isotype control

Related Products

アプリケーション

ターゲット情報

機能

組織特異性

関連疾患

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

参考文献 (123)

レビューと Q&A

IHC - Wholemount abreview for Anti-Sarcomeric Alpha Actinin antibody [EA-53]

Immunohistochemistry (Frozen sections) abreview for Anti-Sarcomeric Alpha Actinin antibody [EA-53]

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Sarcomeric Alpha Actinin antibody [EA-53]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Sarcomeric Alpha Actinin antibody [EA-53]

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Sarcomeric Alpha Actinin antibody [EA-53]

Immunohistochemistry (Frozen sections) abreview for Anti-Sarcomeric Alpha Actinin antibody [EA-53]

Western blot abreview for Anti-Sarcomeric Alpha Actinin antibody [EA-53]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Sarcomeric Alpha Actinin antibody [EA-53]