Anti-SAP97 抗体 (ab3437)
Key features and details
- Rabbit polyclonal to SAP97
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SAP97 antibody
SAP97 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SAP97 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Rat SAP97 aa 115-133.
Sequence:VLPSERISPQVPNEVLGPE
(Peptide available asab5852)
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
IgG fraction -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab3437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | 1/20 - 1/200. | |
| WB | 1/500 - 1/5000. Detects a band of approximately 140 kDa (predicted molecular weight: 140 kDa).Can be blocked with SAP97 peptide (ab5852). |
ターゲット情報
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機能
Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel. -
組織特異性
Abundantly expressed in atrial myocardium (at protein level). Expressed in lung fibroblasts, cervical epithelial and B-cells (at protein level). Widely expressed, with isoforms displaying different expression profiles. -
配列類似性
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 1 L27 domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
ドメイン
The alternatively spliced domain I3 corresponding to amino acids (636-669) of isoform 4 is an EPB41 binding site mediating association to membranes in polarized and non-polarized cells.
The PDZ domains may also mediate association to membranes by binding to EPB41 and ADGRA2 together with the L27 domain that binds CASK and DLG2.
The L27 domain may regulate DLG1 self-association. The N-terminal alternatively spliced region is capable of binding several SH3 domains and also moderates the level of protein oligomerization. -
翻訳後修飾
Phosphorylated by MAPK12. Phosphorylation of Ser-232 regulates association with GRIN2A. -
細胞内局在
Membrane. Basolateral cell membrane. Endoplasmic reticulum membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Cell membrane, sarcolemma. Colocalizes with EPB41 at regions of intercellular contacts. Basolateral in epithelial cells. May also associate with endoplasmic reticulum membranes. Mainly found in neurons soma, moderately found at postsynaptic densities (By similarity). - Information by UniProt
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参照データベース
- Entrez Gene: 1739 Human
- Entrez Gene: 13383 Mouse
- Entrez Gene: 25252 Rat
- Omim: 601014 Human
- SwissProt: Q12959 Human
- SwissProt: Q811D0 Mouse
- SwissProt: Q62696 Rat
- Unigene: 292549 Human
see all -
別名
- Discs large homolog 1 antibody
- discs large, Drosophila, homolog of, 1 antibody
- discs, large homolog 1 (Drosophila) antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody (ab3437)Immunocytochemistry/Immunofluorescent analysis of SAP97 (green) showing staining in the cytoplasm and membrane of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3437 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody (ab3437)Immunocytochemistry/Immunofluorescent analysis of SAP97 (green) showing staining in the cytoplasm and membrane of C2C12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3437 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody (ab3437)Immunocytochemistry/Immunofluorescent analysis of SAP97 (green) showing staining in the cytoplasm and membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3437 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody (ab3437)ICC/IF image of ab3437 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3437, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-SAP97 antibody (ab3437)All lanes : Anti-SAP97 antibody (ab3437) at 1/5000 dilution
Lane 1 : C6 cell lysate
Lane 2 : PC12 cell lysate
Lane 3 : Rat brain cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 140 kDa
プロトコール
データシートおよび資料
参考文献 (6)
ab3437 は 6 報の論文で使用されています。
- Boccitto M et al. Opposing actions of the synapse-associated protein of 97-kDa molecular weight (SAP97) and Disrupted in Schizophrenia 1 (DISC1) on Wnt/ß-catenin signaling. Neuroscience 326:22-30 (2016). PubMed: 27026592
- Didonna A et al. A non-synonymous single-nucleotide polymorphism associated with multiple sclerosis risk affects the EVI5 interactome. Hum Mol Genet 24:7151-8 (2015). PubMed: 26433934
- Underhill SM et al. Differential regulation of two isoforms of the glial glutamate transporter EAAT2 by DLG1 and CaMKII. J Neurosci 35:5260-70 (2015). PubMed: 25834051
- Dolmatova E et al. Cardiomyocyte ATP release through pannexin 1 aids in early fibroblast activation. Am J Physiol Heart Circ Physiol 303:H1208-18 (2012). PubMed: 22982782
- Villa L et al. Effects and mechanisms of angiotensin II receptor blockade with telmisartan in a normotensive model of mesangioproliferative nephritis. Nephrol Dial Transplant 26:3131-43 (2011). PubMed: 21414972
- Cousins SL et al. Differential interaction of NMDA receptor subtypes with the post-synaptic density-95 family of membrane associated guanylate kinase proteins. J Neurochem 104:903-13 (2008). PubMed: 18233995
