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    sap97-antibody-ab3437.pdf

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Anti-SAP97 抗体 (ab3437)

  • Datasheet
Reviews (4)Q&A (1)References (6)

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Abpromise

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Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody (ab3437)
  • Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody (ab3437)
  • Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody (ab3437)
  • Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody (ab3437)
  • Western blot - Anti-SAP97 antibody (ab3437)

Key features and details

  • Rabbit polyclonal to SAP97
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human SAP97 protein (ab152336)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

製品の概要

  • 製品名

    Anti-SAP97 antibody
    SAP97 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to SAP97
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC/IF, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Rat SAP97 aa 115-133.
    Sequence:

    VLPSERISPQVPNEVLGPE


    (Peptide available as ab5852)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度

    IgG fraction
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Adapters
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human SAP97 protein (ab152336)

アプリケーション

Our Abpromise guarantee covers the use of ab3437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/20 - 1/200.
WB 1/500 - 1/5000. Detects a band of approximately 140 kDa (predicted molecular weight: 140 kDa).

ターゲット情報

  • 機能

    Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel.
  • 組織特異性

    Abundantly expressed in atrial myocardium (at protein level). Expressed in lung fibroblasts, cervical epithelial and B-cells (at protein level). Widely expressed, with isoforms displaying different expression profiles.
  • 配列類似性

    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 1 L27 domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • ドメイン

    The alternatively spliced domain I3 corresponding to amino acids (636-669) of isoform 4 is an EPB41 binding site mediating association to membranes in polarized and non-polarized cells.
    The PDZ domains may also mediate association to membranes by binding to EPB41 and ADGRA2 together with the L27 domain that binds CASK and DLG2.
    The L27 domain may regulate DLG1 self-association. The N-terminal alternatively spliced region is capable of binding several SH3 domains and also moderates the level of protein oligomerization.
  • 翻訳後修飾

    Phosphorylated by MAPK12. Phosphorylation of Ser-232 regulates association with GRIN2A.
  • 細胞内局在

    Membrane. Basolateral cell membrane. Endoplasmic reticulum membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Cell membrane, sarcolemma. Colocalizes with EPB41 at regions of intercellular contacts. Basolateral in epithelial cells. May also associate with endoplasmic reticulum membranes. Mainly found in neurons soma, moderately found at postsynaptic densities (By similarity).
  • Target information above from: UniProt accession Q12959 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 1739 Human
    • Entrez Gene: 13383 Mouse
    • Entrez Gene: 25252 Rat
    • Omim: 601014 Human
    • SwissProt: Q12959 Human
    • SwissProt: Q811D0 Mouse
    • SwissProt: Q62696 Rat
    • Unigene: 292549 Human
    • Unigene: 382 Mouse
    • Unigene: 89331 Rat
    see all
  • 別名

    • Discs large homolog 1 antibody
    • discs large, Drosophila, homolog of, 1 antibody
    • discs, large homolog 1 (Drosophila) antibody
    • Disks large homolog 1 antibody
    • dJ1061C18.1.1 antibody
    • DKFZp761P0818 antibody
    • DKFZp781B0426 antibody
    • DLG 1 antibody
    • DLG1 antibody
    • DLG1_HUMAN antibody
    • DLGH 1 antibody
    • DLGH1 antibody
    • hDlg antibody
    • Presynaptic protein SAP97 antibody
    • SAP 97 antibody
    • SAP-97 antibody
    • SAP97 antibody
    • Synapse associated protein 97 antibody
    • Synapse-associated protein 97 antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody (ab3437)
    Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody (ab3437)

    Immunocytochemistry/Immunofluorescent analysis of SAP97 (green) showing staining in the cytoplasm and membrane of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3437 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody (ab3437)
    Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody (ab3437)

    Immunocytochemistry/Immunofluorescent analysis of SAP97 (green) showing staining in the cytoplasm and membrane of C2C12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3437 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody (ab3437)
    Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody (ab3437)

    Immunocytochemistry/Immunofluorescent analysis of SAP97 (green) showing staining in the cytoplasm and membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3437 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody (ab3437)
    Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody (ab3437)
    ICC/IF image of ab3437 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3437, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-SAP97 antibody (ab3437)
    Western blot - Anti-SAP97 antibody (ab3437)
    All lanes : Anti-SAP97 antibody (ab3437) at 1/5000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : Rat brain cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 140 kDa

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
  • 参考文献 (6)

    ab3437 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3437 は 6 報の論文で使用されています。

    • Boccitto M  et al. Opposing actions of the synapse-associated protein of 97-kDa molecular weight (SAP97) and Disrupted in Schizophrenia 1 (DISC1) on Wnt/ß-catenin signaling. Neuroscience 326:22-30 (2016). PubMed: 27026592
    • Didonna A  et al. A non-synonymous single-nucleotide polymorphism associated with multiple sclerosis risk affects the EVI5 interactome. Hum Mol Genet 24:7151-8 (2015). PubMed: 26433934
    • Underhill SM  et al. Differential regulation of two isoforms of the glial glutamate transporter EAAT2 by DLG1 and CaMKII. J Neurosci 35:5260-70 (2015). PubMed: 25834051
    • Dolmatova E  et al. Cardiomyocyte ATP release through pannexin 1 aids in early fibroblast activation. Am J Physiol Heart Circ Physiol 303:H1208-18 (2012). PubMed: 22982782
    • Villa L  et al. Effects and mechanisms of angiotensin II receptor blockade with telmisartan in a normotensive model of mesangioproliferative nephritis. Nephrol Dial Transplant 26:3131-43 (2011). PubMed: 21414972
    • Cousins SL  et al. Differential interaction of NMDA receptor subtypes with the post-synaptic density-95 family of membrane associated guanylate kinase proteins. J Neurochem 104:903-13 (2008). PubMed: 18233995

    レビューと Q&A

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    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-SAP97 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (endothelila HUVEC)
    Gel Running Conditions
    Reduced Denaturing (5-20 gradient)
    Loading amount
    15 µg
    Specification
    endothelila HUVEC
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 May 08 2015

    Western blot abreview for Anti-SAP97 antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Recombinant protein (HEK293)
    Loading amount
    50 µg
    Specification
    HEK293
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 27 2009

    Western blot abreview for Anti-SAP97 antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Recombinant protein (Transfected Cells)
    Loading amount
    50 µg
    Specification
    Transfected Cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 17 2009

    Immunohistochemistry (Frozen sections) abreview for Anti-SAP97 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain sections (30 um free floating))
    Specification
    Brain sections (30 um free floating)
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Read More

    Dr. Sophie Pezet

    Verified customer

    投稿 Dec 06 2007

    Question

    Is a concentration ( at the least, an approximate)available for this antibody? Has it been isotyped beyond IgG?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 02 2004

    Answer

    Unfortunately we have not determined the concentration nor the exact isotype beyond IgG of this antibody.

    Read More

    Abcam Scientific Support

    Answered on Nov 04 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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