Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary neuronal cultures)
Permeabilization
Yes - 0.25% Triton PBS
Specification
Primary neuronal cultures
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA PBS
Dilution
1/200
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
The signal was detected throughout the cells and not limited to dendrites.
Abcam response
Abcam has not tested ab3438 in ICC/IF and does not know how it should perform. The UniProt database (https://www.uniprot.org/uniprot/Q92796) supports localization for this target throughout the cell, including cytosol and plasma membrane.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 11 2022