Key features and details
- Rabbit polyclonal to Sall4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human, Macaque monkey, Common marmoset
- Isotype: IgG
Sall4 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Sall4
アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
種交差性交差種: Mouse, Human, Macaque monkey, Common marmoset
交差が予測される動物種: Chicken, Xenopus laevis
Synthetic peptide conjugated to KLH derived from within residues 1000 to the C-terminus of Mouse Sall4.
- WB: E14tG2a (Mouse embryonic stem cell line) whole cell lysate. IHC-P: Human normal testis tissue. ICC/IF: Mouse embryonic stem cells. IHC-P: Human testis seminoma FFPE tissue sections
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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精製度Immunogen affinity purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab29112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 85, 140 kDa (predicted molecular weight: 66, 113 kDa).|
|IHC-P||Use a concentration of 0.5 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 µg/ml.|
機能Probable transcription factor.
組織特異性Expressed in testis.
関連疾患Defects in SALL4 are the cause of Duane-radial ray syndrome (DRRS) [MIM:607323]; also known as Okihiro syndrome. DRRS is a disorder characterized by the association of forearm malformations with Duane retraction syndrome.
Defects in SALL4 are the cause of oculootoradial syndrome (OORS) [MIM:147750]. Oculootoradial syndrome is an autosomal dominant condition characterized by upper limbs anomalies (radial ray defects, carpal bones fusion), extraocular motor disturbances, congenital bilateral non-progressive mixed hearing loss. Other less consistent malformations include heart involvement, mild thrombocytopenia and leukocytosis (before age 50), shoulder girdle hypoplasia, imperforate anus, kidney malrotation or rectovaginal fistula. The IVIC syndrome is an allelic disorder of Duane-radial ray syndrome (DRRS) with a similar phenotype.
配列類似性Belongs to the sal C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers.
- Information by UniProt
- AA407717 antibody
- AL022809 antibody
- AW536104 antibody
IHC image of Sall4 antibody staining in a section of formalin-fixed paraffin-embedded human testis seminoma* performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab29112, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ICC/IF image of ab29112 stained human embryonic stem cells. The cells were fixed in Paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 10% Goat serum, 0.1% BSA in PBS for 1 hour at RT, before incubation with ab29112 at a 1/100 dilution for 12 hours at 4°C. The secondary used was an Alexa Fluor® 488 conjugated goat anti-rabbit polyclonal, at 1/200 dilution.
All lanes : Anti-Sall4 antibody (ab29112) at 1 µg/ml
Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66, 113 kDa
Observed band size: 140,80 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa (possible non-specific binding)
Exposure time: 8 minutes
MEF1 whole cell lysate was included as a negative control.
IHC-P image of Sall4 staining with ab29112 on tissue sections from a 3 year old adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab29112 (1/100 dilution) for 20 hours at 4°C. The secondary was an Alexa Fluor® 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.
Anti-Sall4 antibody (ab29112) at 1 µg + E14tG2a (Mouse embryonic stem cell line) Whole Cell Lysate at 20 µg
IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 66, 113 kDa
Observed band size: 140,85 kDa why is the actual band size different from the predicted?
This antibody detects two bands which we believe correspond to the two isoforms of Sall4 outlined in Ma et al, 2006 (PMID: 16763212). This paper describes human isoforms running at 165 kDa and 95 kDa. In mouse embryonic stem cells we see both isoforms running at slightly lower molecular weights. We believe that these bands represent Sall4a (Swissprot: Q8BX22) and Sall4b (Swissprot: Q6S7E9) which are predicted to be 113 kDa and 66 kDa respectively.
ICC/IF image of ab29112 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA/10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab29112, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
ab29112 は 54 報の論文で使用されています。
- Peñalosa-Ruiz G et al. The corepressor NCOR1 and OCT4 facilitate early reprogramming by suppressing fibroblast gene expression. PeerJ 8:e8952 (2020). PubMed: 32351783
- Wang B et al. Mouse bone marrow mesenchymal stem cells with distinct p53 statuses display differential characteristics. Mol Med Rep 21:2051-2062 (2020). PubMed: 32186775
- Reimer M et al. Deletion of Tet proteins results in quantitative disparities during ESC differentiation partially attributable to alterations in gene expression. BMC Dev Biol 19:16 (2019). PubMed: 31286885
- Tahara N et al. Sall4 regulates neuromesodermal progenitors and their descendants during body elongation in mouse embryos. Development 146:N/A (2019). PubMed: 31235634
- Legrand JMD et al. DDX5 plays essential transcriptional and post-transcriptional roles in the maintenance and function of spermatogonia. Nat Commun 10:2278 (2019). PubMed: 31123254