Anti-SAE1 抗体 (ab97523)
Key features and details
- Rabbit polyclonal to SAE1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SAE1 antibody
SAE1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SAE1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Recombinant fragment, corresponding to a region within amino acids 3-217 of Human SAE1 (AAH00344)
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ポジティブ･コントロール
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cells, A549 Xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97523の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 38 kDa.
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| IHC-P |
1/100 - 1/250.
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| ICC/IF |
1/100 - 1/200.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 38 kDa. |
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IHC-P
1/100 - 1/250. |
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ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. -
組織特異性
Expression level increases during S phase and drops in G2 phase (at protein level). -
パスウェイ
Protein modification; protein sumoylation. -
配列類似性
Belongs to the ubiquitin-activating E1 family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 505512 Cow
- Entrez Gene: 10055 Human
- Entrez Gene: 56459 Mouse
- Entrez Gene: 308384 Rat
- Omim: 613294 Human
- SwissProt: A2VE14 Cow
- SwissProt: Q9UBE0 Human
- SwissProt: Q9R1T2 Mouse
see all -
別名
- Activator of SUMO1 antibody
- AOS1 antibody
- HSPC140 antibody
see all
画像
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Western blot - Anti-SAE1 antibody (ab97523)All lanes : Anti-SAE1 antibody (ab97523) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 38 kDa
10% SDS-PAGE. -
Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody (ab97523)Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97523 at 1/200 dilution. Lower image is merged with DNA probe. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE1 antibody (ab97523)Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using ab97523 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab97523 は 1 報の論文で使用されています。
- Lapaquette P et al. Shigella entry unveils a calcium/calpain-dependent mechanism for inhibiting sumoylation. Elife 6:N/A (2017). PubMed: 29231810