Anti-SA2 抗体 (ab4464)
Key features and details
- Goat polyclonal to SA2
- Suitable for: WB, ICC
- Reacts with: Human, Xenopus laevis
- Isotype: IgG
製品の概要
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製品名
Anti-SA2 antibody
SA2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to SA2 -
由来種
Goat -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Human, Xenopus laevis
交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide (Human) - 31 amino acids which represent a portion of human SA 2 encoded within exon 31.
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab4464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa). | |
| ICC | 1/500 - 1/2000. |
ターゲット情報
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機能
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. -
配列類似性
Belongs to the SCC3 family.
Contains 1 SCD (stromalin conservative) domain. -
翻訳後修飾
Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation. -
細胞内局在
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. - Information by UniProt
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参照データベース
- Entrez Gene: 10735 Human
- Entrez Gene: 20843 Mouse
- Entrez Gene: 313304 Rat
- Entrez Gene: 394319 Xenopus laevis
- Omim: 300826 Human
- SwissProt: Q8N3U4 Human
- SwissProt: O35638 Mouse
- SwissProt: Q9DGN0 Xenopus laevis
see all -
別名
- bA517O1.1 antibody
- Cohesin Subunit SA 2 antibody
- Cohesin subunit SA-2 antibody
see all
画像
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Immunocytochemistry - Anti-SA2 antibody (ab4464)Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA2 with ab4464 at 1µg/ml.
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Western blot - Anti-SA2 antibody (ab4464)Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4464 at 0.5
µ g/ml.Detection: ECL with 15 second exposure.
Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4464 at 0.5 µg/ml.
Detection: ECL with 15 second exposure.
プロトコール
参考文献 (2)
ab4464 は 2 報の論文で使用されています。
- Faure AJ et al. Cohesin regulates tissue-specific expression by stabilizing highly occupied cis-regulatory modules. Genome Res 22:2163-75 (2012). ChIP-sequencing ; Mouse . PubMed: 22780989
- Inoue A et al. Loss of ChlR1 helicase in mouse causes lethality due to the accumulation of aneuploid cells generated by cohesion defects and placental malformation. Cell Cycle 6:1646-54 (2007). PubMed: 17611414
