Anti-SA2 抗体 (ab4464)

Submit a review Q&A (1)References (3)
Western blot - Anti-SA2 antibody (ab4464)
  • Immunocytochemistry - Anti-SA2 antibody (ab4464)

Key features and details

  • Goat polyclonal to SA2
  • Suitable for: WB, ICC
  • Reacts with: Mouse, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-SA2 antibody
    SA2 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to SA2

  • 由来種

    Goat

  • アプリケーション

    適用あり: WB, ICCmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan

  • 免疫原

    Synthetic peptide (Human) - 31 amino acids which represent a portion of human SA 2 encoded within exon 31.

  • ポジティブ･コントロール

    • WB: HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells. ICC: HeLa cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab4464の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
ICC
1/500 - 1/2000.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
ICC
1/500 - 1/2000.

ターゲット情報

  • 機能

    Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

  • 配列類似性

    Belongs to the SCC3 family.
    Contains 1 SCD (stromalin conservative) domain.

  • 翻訳後修飾

    Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.

  • 細胞内局在

    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • bA517O1.1 antibody
    • Cohesin Subunit SA 2 antibody
    • Cohesin subunit SA-2 antibody
    • DKFZp686P168 antibody
    • DKFZp781H1753 antibody
    • FLJ25871 antibody
    • SA 2 antibody
    • SA-2 antibody
    • SA2 antibody
    • SCC3 homolog 2 antibody
    • SCC3B antibody
    • STAG 2 antibody
    • stag2 antibody
    • STAG2_HUMAN antibody
    • Stromal antigen 2 antibody
    see all

画像

  • Western blot - Anti-SA2 antibody (ab4464)
    Western blot - Anti-SA2 antibody (ab4464)
    All lanes : Anti-SA2 antibody (ab4464) at 1/10000 dilution

    Lane 1 : HeLa Whole cell lysate
    Lane 2 : HEK293T Whole cell lysate
    Lane 3 : Jurkat Whole cell lysate
    Lane 4 : mouse TCMK-1 cells Whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 134 kDa



    Detection: Chemiluminescence with an exposure time of 30 seconds.

  • Immunocytochemistry - Anti-SA2 antibody (ab4464)
    Immunocytochemistry - Anti-SA2 antibody (ab4464)
    Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA2 with ab4464 at 1µg/ml.

参考文献 (3)

ab4464 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab4464 は 3 報の論文で使用されています。

  • Park SH  et al. ATAD5 promotes replication restart by regulating RAD51 and PCNA in response to replication stress. Nat Commun 10:5718 (2019). PubMed: 31844045
  • Faure AJ  et al. Cohesin regulates tissue-specific expression by stabilizing highly occupied cis-regulatory modules. Genome Res 22:2163-75 (2012). ChIP-sequencing ; Mouse . PubMed: 22780989
  • Inoue A  et al. Loss of ChlR1 helicase in mouse causes lethality due to the accumulation of aneuploid cells generated by cohesion defects and placental malformation. Cell Cycle 6:1646-54 (2007). PubMed: 17611414

レビューと Q&A

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Question

I have a question related to the four types of goat anti-SCC3 antibodies that you have (Ab4455, Ab4457, Ab4463, and Ab4464). Do you know which one works best on immunostaining?

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Answer

Thank you for your email. All the information that we have regarding these antibodies is located on the online datasheets. I unfortunately can't tell you which one works "best." Are you detecting SA1 or SA2? The differences between the two SA1 antibodies and the two SA2 antibodies are the immunogens. The antibodies are fairly new and we have not had feedback from customers yet. If you have any more questions, please contact us again.

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