Anti-SA1 抗体 (ab4457)
Key features and details
- Goat polyclonal to SA1
- Suitable for: ChIP, WB, ICC, IP, ChIP/Chip
- Reacts with: Mouse, Human, Xenopus laevis
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SA1 antibody
SA1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to SA1 -
由来種
Goat -
アプリケーション
適用あり: ChIP, WB, ICC, IP, ChIP/Chipmore details -
種交差性
交差種: Mouse, Human, Xenopus laevis
交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Human SA1 aa 1150-1200.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4457の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ChIP |
Use at an assay dependent concentration. PubMed: 18550811
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WB |
1/2000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).
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ICC |
1/500 - 1/2000.
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IP |
Use at 2-10 µg/mg of lysate.
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ChIP/Chip |
Use at an assay dependent concentration. PubMed: 18550811
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特記事項 |
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ChIP
Use at an assay dependent concentration. PubMed: 18550811 |
WB
1/2000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa). |
ICC
1/500 - 1/2000. |
IP
Use at 2-10 µg/mg of lysate. |
ChIP/Chip
Use at an assay dependent concentration. PubMed: 18550811 |
ターゲット情報
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機能
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. -
配列類似性
Belongs to the SCC3 family.
Contains 1 SCD (stromalin conservative) domain. -
翻訳後修飾
Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation. -
細胞内局在
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. - Information by UniProt
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参照データベース
- Entrez Gene: 10274 Human
- Entrez Gene: 20842 Mouse
- Entrez Gene: 399163 Xenopus laevis
- Omim: 604358 Human
- SwissProt: Q8WVM7 Human
- SwissProt: Q9D3E6 Mouse
- SwissProt: Q9DGN1 Xenopus laevis
- Unigene: 412586 Human
see all -
別名
- Cohesin subunit SA 1 antibody
- Cohesin subunit SA-1 antibody
- Cohesin Subunit SA1 antibody
see all
画像
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All lanes : Anti-SA1 antibody (ab4457) at 1/10000 dilution
Lane 1 : HeLa Whole cell lysate
Lane 2 : 293T Whole cell lysate
Lane 3 : Jurkat Whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 144 kDaDetection: Chemiluminescence with an exposure time of 30 seconds.
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SA1 was immunoprecipitated from HeLa whole cell lysate with 6 µg/mg lysate ab4457. Western blot was performed from the immunoprecipitate using ab4457 at 0.1 µg/ml dilution.
Lane 1: ab4457 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
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Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA1 with ab4457 at 1µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab4457 は 17 報の論文で使用されています。
- Morgan SM et al. The three-dimensional structure of Epstein-Barr virus genome varies by latency type and is regulated by PARP1 enzymatic activity. Nat Commun 13:187 (2022). PubMed: 35039491
- Luo H et al. HOTTIP-dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia. Mol Cell 82:833-851.e11 (2022). PubMed: 35180428
- Choi EH et al. Meiosis-specific cohesin complexes display essential and distinct roles in mitotic embryonic stem cell chromosomes. Genome Biol 23:70 (2022). PubMed: 35241136
- Chu Z et al. STAG2 regulates interferon signaling in melanoma via enhancer loop reprogramming. Nat Commun 13:1859 (2022). PubMed: 35388001
- Wang J et al. Large-scale multi-omics analysis suggests specific roles for intragenic cohesin in transcriptional regulation. Nat Commun 13:3218 (2022). PubMed: 35680859