Key features and details
- Goat polyclonal to SA1
- Suitable for: WB, ICC
- Reacts with: Human, Xenopus laevis
- Isotype: IgG
SA1 一次抗体 製品一覧
製品の詳細Goat polyclonal to SA1
アプリケーション適用あり: WB, ICCmore details
種交差性交差種: Human, Xenopus laevis
交差が予測される動物種: Mouse, Rhesus monkey
Synthetic peptide within Human SA1 aa 25-75. The exact sequence is proprietary.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab4455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).|
|ICC||1/500 - 1/2000.|
機能Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
配列類似性Belongs to the SCC3 family.
Contains 1 SCD (stromalin conservative) domain.
翻訳後修飾Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
細胞内局在Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
- Information by UniProt
- Cohesin subunit SA 1 antibody
- Cohesin subunit SA-1 antibody
- Cohesin Subunit SA1 antibody
Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4455 at 0.5
Detection: ECL with 15 second exposure.Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4455 at 0.5 µg/ml.
Detection: ECL with 15 second exposure.
Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA1 with ab4455 at 1µg/ml.
ab4455 は 3 報の論文で使用されています。
- Hopkins J et al. Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomes. PLoS Genet 10:e1004413 (2014). WB ; Mouse . PubMed: 24992337
- Xiao T et al. Specific Sites in the C Terminus of CTCF Interact with the SA2 Subunit of the Cohesin Complex and Are Required for Cohesin-Dependent Insulation Activity. Mol Cell Biol 31:2174-83 (2011). WB ; Human . PubMed: 21444719
- Leman AR et al. Human Timeless and Tipin stabilize replication forks and facilitate sister-chromatid cohesion. J Cell Sci 123:660-70 (2010). WB, IP ; Human . PubMed: 20124417