Anti-S6K1 (phospho T229) 抗体 (ab5231)

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Western blot - Anti-S6K1 (phospho T229) antibody (ab5231)

    Key features and details

    • Rabbit polyclonal to S6K1 (phospho T229)
    • Suitable for: WB
    • Reacts with: Rat, Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-S6K1 (phospho T229) antibody
      S6K1 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to S6K1 (phospho T229)

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Rat, Human

    • 免疫原

      Other Immunogen Type corresponding to Human S6K1. The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human p70-S6K that contains threonine 229. The sequence is conserved in rat.

    • ポジティブ･コントロール

      • Hek293 cells +/- EGF; truncated p70S6K protein, an N-terminus His-tagged fusion protein corresponding to amino acids 1-421 of human p70S6K containing a T412E mutation to become constitutively active.

    • 特記事項

      70 kDa ribosomal protein S6 kinase (p70S6K or p70S6K beta 1) is a member of a serine/threonine kinase family that phosphorylates the 40S ribosomal protein S6, thereby modulating the translation of ribosomal proteins and translation elongation factors. p70S6K is activated in response to the phosphatidylinositol 3-kinase (PI3K) and mitogen-activated protein kinase (MAPK) pathways, and is required for progression through the cell cycle and for cell growth. Activation of p70S6K is regulated by phosphorylation of seven different residues distributed throughout the protein, with the critical sites being threonines 229, 389, 421 and serine 424. Threonine 229 is located within the activation loop.

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    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • バッファー

      pH: 7.30
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 0.1% BSA

      BSA is IgG and protease free
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated p70S6K. The final product is generated by affinity chromatography using a p70S6K derived peptide that is phosphorylated at threonine 229.

    • 一次抗体 備考

      70 kDa ribosomal protein S6 kinase (p70S6K or p70S6K beta 1) is a member of a serine/threonine kinase family that phosphorylates the 40S ribosomal protein S6, thereby modulating the translation of ribosomal proteins and translation elongation factors. p70S6K is activated in response to the phosphatidylinositol 3-kinase (PI3K) and mitogen-activated protein kinase (MAPK) pathways, and is required for progression through the cell cycle and for cell growth. Activation of p70S6K is regulated by phosphorylation of seven different residues distributed throughout the protein, with the critical sites being threonines 229, 389, 421 and serine 424. Threonine 229 is located within the activation loop.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab5231 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 70 kDa.

    ターゲット情報

    • 機能

      Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.

    • 組織特異性

      Widely expressed.

    • 配列類似性

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 protein kinase domain.

    • ドメイン

      The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
      The TOS (TOR signaling) motif is essential for activation by mTORC1.

    • 翻訳後修飾

      Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism.

    • 細胞内局在

      Cytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • 70 kDa ribosomal protein S6 kinase 1 antibody
        • KS6B1_HUMAN antibody
        • p70 alpha antibody
        • P70 beta 1 antibody
        • p70 ribosomal S6 kinase alpha antibody
        • p70 ribosomal S6 kinase beta 1 antibody
        • p70 S6 kinase alpha antibody
        • P70 S6 Kinase antibody
        • p70 S6 kinase, alpha 1 antibody
        • p70 S6 kinase, alpha 2 antibody
        • p70 S6K antibody
        • p70 S6K-alpha antibody
        • p70 S6KA antibody
        • p70(S6K) alpha antibody
        • p70(S6K)-alpha antibody
        • p70-alpha antibody
        • p70-S6K 1 antibody
        • p70-S6K antibody
        • P70S6K antibody
        • P70S6K1 antibody
        • p70S6Kb antibody
        • PS6K antibody
        • Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody
        • Ribosomal protein S6 kinase beta 1 antibody
        • Ribosomal protein S6 kinase beta-1 antibody
        • Ribosomal protein S6 kinase I antibody
        • RPS6KB1 antibody
        • S6K antibody
        • S6K-beta-1 antibody
        • S6K1 antibody
        • Serine/threonine kinase 14 alpha antibody
        • Serine/threonine-protein kinase 14A antibody
        • STK14A antibody
        see all

      画像

      • Western blot - Anti-S6K1 (phospho T229) antibody (ab5231)
        Western blot - Anti-S6K1 (phospho T229) antibody (ab5231)
        Extracts prepared from Hek293 cells (1-4) or activated p70 S6 kinase protein (5, 6) were resolved on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either untreated (1-5), or treated with lambda (ë) phosphatase (6), blocked with a 5% BSA-TBST buffer overnight at 4°C, then incubated with 0.50 µg/mL ab5231  for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: nopeptide (1, 5, 6), the non-phosphorylated peptide corresponding to the phosphopeptide (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4).After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and the signal was detected using the Tropix WesternStar method.The data show that only the phosphopeptide corresponding to p70S6K [pT229] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the si

      参考文献 (11)

      ab5231 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab5231 は 11 報の論文で使用されています。

      • Chen S  et al. Salidroside protects SH-SY5Y from pathogenic a-synuclein by promoting cell autophagy via mediation of mTOR/p70S6K signaling. Mol Med Rep 20:529-538 (2019). PubMed: 31180515
      • Silva RAC  et al. Cardiac Remodeling Induced by All-Trans Retinoic Acid is Detrimental in Normal Rats. Cell Physiol Biochem 43:1449-1459 (2017). PubMed: 29017174
      • Ke K & Lou T MicroRNA-10a suppresses breast cancer progression via PI3K/Akt/mTOR pathway. Oncol Lett 14:5994-6000 (2017). PubMed: 29113237
      • Boyer A  et al. mTOR Regulates Gap Junction Alpha-1 Protein Trafficking in Sertoli Cells and Is Required for the Maintenance of Spermatogenesis in Mice. Biol Reprod 95:13 (2016). PubMed: 27281705
      • Gao B  et al. Eicosapentaenoic acid attenuates dexamethasome-induced apoptosis by inducing adaptive autophagy via GPR120 in murine bone marrow-derived mesenchymal stem cells. Cell Death Dis 7:e2235 (2016). WB ; Mouse . PubMed: 27228350
      View all Publications for this product

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      Question

      Do you supply the positive control to go with ab5231 anti-S6K1 (phospho T229)?

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      Answer

      We do supply the positive control for the Anti-S6K1 (phospho T229) antibody (ab5231).


      The positive control is HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902). Please refer to the Cell Lysate datasheet for further information.


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      Question

      Customer kindly contacted us as the product was not phospho specific in their experiments.

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      Answer

      Thank you for contacting us. Your credit note ID is xxxxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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