Anti-S6K1 抗体 (ab14708)
Key features and details
- Goat polyclonal to S6K1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-S6K1 antibody
S6K1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to S6K1 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Cow, Dog, Xenopus laevis
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免疫原
Synthetic peptide: MISKRPEHLRMNL, corresponding to amino acids 490-502 of S6K1.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14708の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.05 - 0.1 µg/ml. Detects a band of approximately 65-70 kDa (predicted molecular weight: 60 kDa).
A 1 hour primary incubation is recommended for this product. |
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| IHC-P |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.05 - 0.1 µg/ml. Detects a band of approximately 65-70 kDa (predicted molecular weight: 60 kDa). A 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. -
ドメイン
The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
The TOS (TOR signaling) motif is essential for activation by mTORC1. -
翻訳後修飾
Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism. -
細胞内局在
Cytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 404181 Cow
- Entrez Gene: 6198 Human
- Entrez Gene: 72508 Mouse
- Entrez Gene: 100009260 Rabbit
- Entrez Gene: 83840 Rat
- Omim: 608938 Human
- SwissProt: P23443 Human
- SwissProt: Q8BSK8 Mouse
see all -
別名
- 70 kDa ribosomal protein S6 kinase 1 antibody
- KS6B1_HUMAN antibody
- p70 alpha antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 antibody (ab14708)
ab14708 (2µg/ml) staining of paraffin embedded Human Kidney shows staining in the cytoplasm-rich PCT while less so in the DCT and no signals in the glomerulus. following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining.
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Western blot - Anti-S6K1 antibody (ab14708)Anti-S6K1 antibody (ab14708) at 1 µg/ml + NIH3T3 cell lysate (in RIPA buffer). at 35 µg
Predicted band size: 60 kDaDetected by chemiluminescence.
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Western blot - Anti-S6K1 antibody (ab14708)Anti-S6K1 antibody (ab14708) at 0.3 µg/ml + Human Placenta lysate (in RIPA buffer) at 35 µg
Predicted band size: 60 kDaDetected by chemiluminescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (4)
ab14708 は 4 報の論文で使用されています。
- Zhang J & Zhang C Silence of long non-coding RNA UCA1 inhibits hemangioma cells growth, migration and invasion by up-regulation of miR-200c. Life Sci 226:33-46 (2019). PubMed: 30898646
- Arif A et al. Multisite Phosphorylation of S6K1 Directs a Kinase Phospho-code that Determines Substrate Selection. Mol Cell 73:446-457.e6 (2019). PubMed: 30612880
- Yang H et al. Inhibition of Gli1-mediated prostate cancer cell proliferation by inhibiting the mTOR/S6K1 signaling pathway. Oncol Lett 14:7970-7976 (2017). PubMed: 29250185
- Azizoglu DB et al. Vascular development in the vertebrate pancreas. Dev Biol 420:67-78 (2016). PubMed: 27789228
