Anti-S100A9 + Calprotectin (S100A8/A9 complex) 抗体 [MAC387] (ab22506)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)](https://www.abcam.co.jp/ps/products/22/ab22506/Images/ab22506-260173-anti-s100a9-calprotectin-s100a8-a9-complex-antibody-mac387-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)")
Key features and details
- Mouse monoclonal [MAC387] to S100A9 + Calprotectin (S100A8/A9 complex)
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387]
S100A9 + Calprotectin (S100A8/A9 complex) 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MAC387] to S100A9 + Calprotectin (S100A8/A9 complex) -
由来種
Mouse -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Monkey, Baboon
非交差種:
Rat -
免疫原
Tissue, cells or virus corresponding to Human S100A9 + Calprotectin (S100A8/A9 complex). Affinity purified solubilized human monocyte preparation.
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ポジティブ･コントロール
- IHC-P: Mouse spleen tissue; human Hodgkin's lymphoma tissue.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
MAC387 -
ミエローマ
NS1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab22506の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (17) |
Use a concentration of 1 µg/ml.
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 1 µg/ml. |
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)This image is courtesy of an Abreview submitted by Elizabeth Chlipala
ab22506 staining Macrophages in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with 0.250% casein for 5 minutes at 25°C; antigen retrieval was by proteinase K. Samples were incubated with primary antibody (1/5000 in Tris-HCL) for 30 minutes at 25°C. A HRP-conjugated labeled polymer was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)](https://www.abcam.co.jp/ps/products/22/ab22506/Images/ab22506-31854-anti-s100a9-calprotectin-s100a8-a9-complex-antibody-mac387-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)IHC image of Macrophage staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)](https://www.abcam.co.jp/ps/products/22/ab22506/Images/ab22506-31856-anti-s100a9-calprotectin-s100a8-a9-complex-antibody-mac387-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)IHC image of Macrophage staining in human Hodgkins lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (74)
ab22506 は 74 報の論文で使用されています。
- Taylor SA et al. Transcriptional profiling of pediatric cholestatic livers identifies three distinct macrophage populations. PLoS One 16:e0244743 (2021). PubMed: 33411796
- Cassimeris L et al. Interleukin-17A pathway target genes are upregulated in Equus caballus supporting limb laminitis. PLoS One 15:e0232920 (2020). PubMed: 33301461
- Wang Z et al. Mfsd2a and Spns2 are essential for sphingosine-1-phosphate transport in the formation and maintenance of the blood-brain barrier. Sci Adv 6:eaay8627 (2020). PubMed: 32523984
- Moon BF et al. Iron imaging in myocardial infarction reperfusion injury. Nat Commun 11:3273 (2020). PubMed: 32601301
- Ecke TH et al. Prognostic Role of Survivin and Macrophage Infiltration Quantified on Protein and mRNA Level in Molecular Subtypes Determined by RT-qPCR of KRT5, KRT20, and ERBB2 in Muscle-Invasive Bladder Cancer Treated by Adjuvant Chemotherapy. Int J Mol Sci 21:N/A (2020). PubMed: 33050010