リコンビナントRabMAb

Anti-S100A12/CGRP 抗体 [EPR23677-111] (ab272713)

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Western blot - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
  • Immunocytochemistry/ Immunofluorescence - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
  • Flow Cytometry - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
  • Immunoprecipitation - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
  • Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23677-111] to S100A12/CGRP
  • Suitable for: WB, IHC-P, Flow Cyt, IP, ICC/IF
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-S100A12/CGRP antibody [EPR23677-111]
    S100A12/CGRP 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23677-111] to S100A12/CGRP

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human spleen and lymph node tissue lysate. IHC-P: Human spleen tissue. Human bladder cancer and cervical cancer tissue. ICC/IF: Human PBMCs. Flow Cyt: Human PBMCs. IP: Human lymph node tissue lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
IP
Human
WB
Human
アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 10 kDa.
IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
1/400.
IP
1/30.
ICC/IF
1/100.
特記事項
WB
1/1000. Predicted molecular weight: 10 kDa.
IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
1/400.
IP
1/30.
ICC/IF
1/100.

ターゲット情報

  • 機能

    Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus. Binds calcium, zinc and copper. Presence of zinc increases the affinity for calcium. Plays an important role in the inflammatory response. Interaction with AGER on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators.

  • 組織特異性

    Monocytes and lymphocytes.

  • 配列類似性

    Belongs to the S-101 family.
    Contains 2 EF-hand domains.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CAAF1 antibody
      • CAGC antibody
      • Calcitermin antibody
      • Calcium-binding protein in amniotic fluid 1 antibody
      • Calgranulin C antibody
      • Calgranulin-C antibody
      • Calgranulin-related protein antibody
      • CGRP antibody
      • EN RAGE antibody
      • EN-RAGE antibody
      • ENRAGE antibody
      • Extracellular newly identified RAGE-binding protein antibody
      • migration inhibitory factor-related protein 6 antibody
      • MRP6 antibody
      • Neutrophil S100 protein antibody
      • p6 antibody
      • Protein S100 A12 antibody
      • S100 calcium binding protein A12 antibody
      • S100 calcium-binding protein A12 (calgranulin C) antibody
      • S100 calcium-binding protein A12 antibody
      • S100A12 antibody
      • S10AC_HUMAN antibody
      see all

    画像

    • Western blot - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
      Western blot - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
      All lanes : Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713) at 1/1000 dilution

      Lane 1 : Human spleen tissue lysate
      Lane 2 : Human lymph node tissue lysate
      Lane 3 : Human brain tissue lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

      Predicted band size: 10 kDa
      Observed band size: 10 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 19542470).

      Negative control: Human brain (PMID: 8718672).

      Exposure time: 103 seconds.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

      Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling S100A12/CGRP with ab272713 at 1/5000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulocytes of human spleen (PMID: 10464253). The section was incubated with ab272713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunocytochemistry/ Immunofluorescence - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
      Immunocytochemistry/ Immunofluorescence - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized human PBMC cells labelling S100A12/CGRP with ab272713 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing cytoplasmic staining in subsets of human PBMC cell line. ab195889 anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 µg/ml dilution.

    • Flow Cytometry - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
      Flow Cytometry - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

      Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized human peripheral blood mononuclear cell (PBMC) cells labeling S100A12/CGRP with ab272713 at 1/400 dilution (0.1µg) (Right panel), compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left panel). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    • Immunoprecipitation - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
      Immunoprecipitation - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

      S100A12/CGRP was immunoprecipitated from 0.35 mg human lymph node tissue lysate 10 µg with ab272713 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272713 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Human lymph node tissue lysate 10 µg.

      Lane 2: ab272713 IP in Human lymph node tissue lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272713 in human lymph node tissue lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 50 seconds.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

      Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling S100A12/CGRP with ab272713 at 1/5000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulocytes of human bladder cancer (PMID: 31993369). The section was incubated with ab272713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

      Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling S100A12/CGRP with ab272713 at 1/5000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulocytes of human cervical cancer (PMID: 31993369). The section was incubated with ab272713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
      Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272713 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272713 は論文での使用が確認できていません。

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