Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Nerve)
Specification
Nerve
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid
Permeabilization
No
Blocking step
non fat milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 4°C
Other product details
Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.02M PBS/1.5% non-fat milk
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-mouse IgG Alexa Fluor (R) 594
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 594
Dilution
1/250
Additional data
Additional Notes
This image shows "Rat Urinary Bladder". Red indicates S100 antibody-positive "Nerve Structures". Green and Blue indicate uroplakin III antibody-positive "Urothelium" and "Nuclei" stained with DAPI, respectively.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. 哲也 今村
Verified customer
投稿 Jul 17 2008