製品名Anti-S100 antibody - Astrocyte Marker, prediluted
S100 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to S100 - Astrocyte Marker, prediluted
特異性This antibody reacts with cow S-100 protein A and B. It shows strong cross reactivity with human S-100 A and B. In the brain, it labels glial and ependymal cells. Melanocytes and Langerhans cells of the skin are also stained with this antibody.
アプリケーション適用あり: IHC-P, IHC-Frmore details
種交差性交差種: Cow, Human
Affinity purified S-100 protein isolated from cow brain.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
バッファーPreservative: 0.05% Sodium azide
Concentration information loading...
精製度Protein A purified
Our Abpromise guarantee covers the use of ab948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user.
Proteolytic digestion of tissue section is required prior to staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
組織特異性Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
配列類似性Belongs to the S-100 family.
Contains 2 EF-hand domains.
- Information by UniProt
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
Ab948 staining human normal skin. Staining is localised to nuclear and cytoplasmic compartments.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH6 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may b
This product has been referenced in:
- Ding L et al. GABA(A) receptor alpha1 subunit mutation A322D associated with autosomal dominant juvenile myoclonic epilepsy reduces the expression and alters the composition of wild type GABA(A) receptors. J Biol Chem 285:26390-405 (2010). Read more (PubMed: 20551311) »